SrasV1, Main, Exploration, bibRecordById, pubmed:16081995

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Identifieur interne : 004D83 ( Main/Exploration ); précédent : 004D82; suivant : 004D84

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham [Singapour] ; LEE KOK KEONG [Singapour] ; LIM SENG GEE [Singapour] ; HONG WANJIN [Singapour]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.

RBID : Pascal:05-0367924

Descripteurs français

KwdFr :
- Humains, RT-PCR (), Sensibilité et spécificité, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
Pascal (Inist)
- Coronavirus, Humains, RT-PCR, Réaction chaîne polymérase RT, Sensibilité et spécificité, Temps réel, Détection, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Detection, Humans, Microbiology, Real time, Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse transcription polymerase chain reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe acute respiratory syndrome.
MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Sensitivity and Specificity.

Abstract

Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.

Affiliations:

Singapour

Le document en format XML

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<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.</div>
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Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration SRAS
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