Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.
Identifieur interne : 001096 ( Ncbi/Curation ); précédent : 001095; suivant : 001097Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.
Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham ; Lee Kok Keong ; Lim Seng Gee ; Hong WanjinSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Humains, RT-PCR, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Sensitivity and Specificity.
Abstract
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.
DOI: 10.1128/JCM.43.8.4262-4265.2005
PubMed: 16081995
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pubmed:16081995Le document en format XML
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uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name></author><author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16081995</idno><idno type="pmid">16081995</idno><idno type="doi">10.1128/JCM.43.8.4262-4265.2005</idno><idno type="wicri:Area/PubMed/Corpus">002604</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002604</idno><idno type="wicri:Area/PubMed/Curation">002604</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002604</idno><idno type="wicri:Area/PubMed/Checkpoint">002586</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002586</idno><idno type="wicri:Area/Ncbi/Merge">001096</idno><idno 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Keong</name></author><author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name></author><author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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