Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Identifieur interne : 000626 ( PascalFrancis/Corpus ); précédent : 000625; suivant : 000627Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Auteurs : Masafumi Inoue ; Timothy Barkham ; LEE KOK KEONG ; LIM SENG GEE ; HONG WANJINSource :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 05-0367924 INIST |
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ET : | Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus |
AU : | INOUE (Masafumi); BARKHAM (Timothy); LEE KOK KEONG; LIM SENG GEE; HONG WANJIN |
AF : | Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 3 aut., 4 aut., 5 aut.); Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng/Singapore 3084332/Singapour (2 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of clinical microbiology : (Print); ISSN 0095-1137; Coden JCMIDW; Etats-Unis; Da. 2005; Vol. 43; No. 8; Pp. 4262-4265; Bibl. 19 ref. |
LA : | Anglais |
EA : | Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE. |
CC : | 002A05C10; 002B05 |
FD : | Coronavirus; Réaction chaîne polymérase RT; Temps réel; Détection; Microbiologie; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie |
ED : | Coronavirus; Reverse transcription polymerase chain reaction; Real time; Detection; Microbiology; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Coronavirus; Reacción cadena polimerasa transcripción inversa; Tiempo real; Detección; Microbiología; Síndrome respiratorio agudo severo |
LO : | INIST-17088.354000131508681120 |
ID : | 05-0367924 |
Links to Exploration step
Pascal:05-0367924Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</title>
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<ET>Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</ET>
<AU>INOUE (Masafumi); BARKHAM (Timothy); LEE KOK KEONG; LIM SENG GEE; HONG WANJIN</AU>
<AF>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 3 aut., 4 aut., 5 aut.); Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng/Singapore 3084332/Singapour (2 aut.)</AF>
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<EA>Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.</EA>
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