SrasV1, PubMed, Corpus, bibRecord, 002604

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.

Identifieur interne : 002604 ( PubMed/Corpus ); précédent : 002603; suivant : 002605

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.

Auteurs : Masafumi Inoue ; Timothy Barkham ; Lee Kok Keong ; Lim Seng Gee ; Hong Wanjin

Source :

Journal of clinical microbiology [ 0095-1137 ] ; 2005.

RBID : pubmed:16081995

English descriptors

KwdEn :
- Humans, Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity.
MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Sensitivity and Specificity.

Abstract

Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.

DOI: 10.1128/JCM.43.8.4262-4265.2005
PubMed: 16081995

Links to Exploration step

pubmed:16081995

Le document en format XML

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<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
</author>
<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
</author>
<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
</author>
<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
</author>
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<affiliation><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
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<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
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<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
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<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
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<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
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<series><title level="j">Journal of clinical microbiology</title>
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<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</div>
</front>
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<DateCompleted><Year>2005</Year>
<Month>09</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN>
<JournalIssue CitedMedium="Print"><Volume>43</Volume>
<Issue>8</Issue>
<PubDate><Year>2005</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Journal of clinical microbiology</Title>
<ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</ArticleTitle>
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<Abstract><AbstractText>Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</AbstractText>
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<ReferenceList><Reference><Citation>Clin Chem. 2003 Jun;49(6 Pt 1):953-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12765993</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 May 24;361(9371):1779-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781537</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Apr;42(4):1471-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15070991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):1956-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131154</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):1994-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131160</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):2043-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131168</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):2094-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2004 Jun;50(6):1050-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15054079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2004 Sep 1;120(1):33-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15234807</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2004 Dec 1;122(1):29-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15488617</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 1996 May;30(2-3):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8783804</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2003 Jun;49(6 Pt 1):845-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12765977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2000 Jun 15;28(12):E63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10871386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
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Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki