Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.
Identifieur interne : 002604 ( PubMed/Curation ); précédent : 002603; suivant : 002605Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.
Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham ; Lee Kok Keong ; Lim Seng Gee ; Hong WanjinSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Humains, RT-PCR, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Sensitivity and Specificity.
Abstract
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.
DOI: 10.1128/JCM.43.8.4262-4265.2005
PubMed: 16081995
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002604
Links to Exploration step
pubmed:16081995Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
</author>
<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
</author>
<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
</author>
<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16081995</idno>
<idno type="pmid">16081995</idno>
<idno type="doi">10.1128/JCM.43.8.4262-4265.2005</idno>
<idno type="wicri:Area/PubMed/Corpus">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002604</idno>
<idno type="wicri:Area/PubMed/Curation">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002604</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
</author>
<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
</author>
<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
</author>
<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
</author>
</analytic>
<series><title level="j">Journal of clinical microbiology</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Humains</term>
<term>RT-PCR</term>
<term>Sensibilité et spécificité</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16081995</PMID>
<DateCompleted><Year>2005</Year>
<Month>09</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN>
<JournalIssue CitedMedium="Print"><Volume>43</Volume>
<Issue>8</Issue>
<PubDate><Year>2005</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Journal of clinical microbiology</Title>
<ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</ArticleTitle>
<Pagination><MedlinePgn>4262-5</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Inoue</LastName>
<ForeName>Masafumi</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Barkham</LastName>
<ForeName>Timothy</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Keong</LastName>
<ForeName>Lee Kok</ForeName>
<Initials>LK</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gee</LastName>
<ForeName>Lim Seng</ForeName>
<Initials>LS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wanjin</LastName>
<ForeName>Hong</ForeName>
<Initials>H</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Clin Microbiol</MedlineTA>
<NlmUniqueID>7505564</NlmUniqueID>
<ISSNLinking>0095-1137</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>8</Month>
<Day>6</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>9</Month>
<Day>16</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>8</Month>
<Day>6</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16081995</ArticleId>
<ArticleId IdType="pii">43/8/4262</ArticleId>
<ArticleId IdType="doi">10.1128/JCM.43.8.4262-4265.2005</ArticleId>
<ArticleId IdType="pmc">PMC1233944</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Clin Chem. 2003 Jun;49(6 Pt 1):953-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12765993</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 May 24;361(9371):1779-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781537</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Apr;42(4):1471-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15070991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):1956-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131154</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):1994-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131160</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):2043-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131168</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 May;42(5):2094-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2004 Jun;50(6):1050-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15054079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2004 Sep 1;120(1):33-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15234807</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2004 Dec 1;122(1):29-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15488617</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 1996 May;30(2-3):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8783804</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2003 Jun;49(6 Pt 1):845-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12765977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2000 Jun 15;28(12):E63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10871386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002604 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002604 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:16081995 |texte= Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:16081995" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |