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Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Identifieur interne : 000364 ( PascalFrancis/Curation ); précédent : 000363; suivant : 000365

Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham [Singapour] ; LEE KOK KEONG [Singapour] ; LIM SENG GEE [Singapour] ; HONG WANJIN [Singapour]

Source :

RBID : Pascal:05-0367924

Descripteurs français

English descriptors

Abstract

Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.
pA  
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A02 01      @0 JCMIDW
A03   1    @0 J. clin. microbiol. : (Print)
A05       @2 43
A06       @2 8
A08 01  1  ENG  @1 Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
A11 01  1    @1 INOUE (Masafumi)
A11 02  1    @1 BARKHAM (Timothy)
A11 03  1    @1 LEE KOK KEONG
A11 04  1    @1 LIM SENG GEE
A11 05  1    @1 HONG WANJIN
A14 01      @1 Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos @2 Singapore 138673 @3 SGP @Z 1 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut.
A14 02      @1 Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng @2 Singapore 3084332 @3 SGP @Z 2 aut.
A20       @1 4262-4265
A21       @1 2005
A23 01      @0 ENG
A43 01      @1 INIST @2 17088 @5 354000131508681120
A44       @0 0000 @1 © 2005 INIST-CNRS. All rights reserved.
A45       @0 19 ref.
A47 01  1    @0 05-0367924
A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of clinical microbiology : (Print)
A66 01      @0 USA
C01 01    ENG  @0 Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.
C02 01  X    @0 002A05C10
C02 02  X    @0 002B05
C03 01  X  FRE  @0 Coronavirus @2 NW @5 01
C03 01  X  ENG  @0 Coronavirus @2 NW @5 01
C03 01  X  SPA  @0 Coronavirus @2 NW @5 01
C03 02  X  FRE  @0 Réaction chaîne polymérase RT @5 05
C03 02  X  ENG  @0 Reverse transcription polymerase chain reaction @5 05
C03 02  X  SPA  @0 Reacción cadena polimerasa transcripción inversa @5 05
C03 03  X  FRE  @0 Temps réel @5 06
C03 03  X  ENG  @0 Real time @5 06
C03 03  X  SPA  @0 Tiempo real @5 06
C03 04  X  FRE  @0 Détection @5 07
C03 04  X  ENG  @0 Detection @5 07
C03 04  X  SPA  @0 Detección @5 07
C03 05  X  FRE  @0 Microbiologie @5 08
C03 05  X  ENG  @0 Microbiology @5 08
C03 05  X  SPA  @0 Microbiología @5 08
C03 06  X  FRE  @0 Syndrome respiratoire aigu sévère @2 NM @5 14
C03 06  X  ENG  @0 Severe acute respiratory syndrome @2 NM @5 14
C03 06  X  SPA  @0 Síndrome respiratorio agudo severo @2 NM @5 14
C07 01  X  FRE  @0 Coronaviridae @2 NW
C07 01  X  ENG  @0 Coronaviridae @2 NW
C07 01  X  SPA  @0 Coronaviridae @2 NW
C07 02  X  FRE  @0 Nidovirales @2 NW
C07 02  X  ENG  @0 Nidovirales @2 NW
C07 02  X  SPA  @0 Nidovirales @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Appareil respiratoire pathologie @5 13
C07 04  X  ENG  @0 Respiratory disease @5 13
C07 04  X  SPA  @0 Aparato respiratorio patología @5 13
C07 05  X  FRE  @0 Virose
C07 05  X  ENG  @0 Viral disease
C07 05  X  SPA  @0 Virosis
C07 06  X  FRE  @0 Infection
C07 06  X  ENG  @0 Infection
C07 06  X  SPA  @0 Infección
C07 07  X  FRE  @0 Poumon pathologie @5 16
C07 07  X  ENG  @0 Lung disease @5 16
C07 07  X  SPA  @0 Pulmón patología @5 16
N21       @1 255
N44 01      @1 OTO
N82       @1 OTO

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Pascal:05-0367924

Le document en format XML

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   |texte=   Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
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