Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Identifieur interne : 004D83 ( Main/Curation ); précédent : 004D82; suivant : 004D84Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham [Singapour] ; LEE KOK KEONG [Singapour] ; LIM SENG GEE [Singapour] ; HONG WANJIN [Singapour]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Pascal (Inist)
English descriptors
- KwdEn :
- MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Sensitivity and Specificity.
Abstract
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000626
- to stream PascalFrancis, to step Curation: Pour aller vers cette notice dans l'étape Curation :000364
- to stream PascalFrancis, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000600
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :005120
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002604
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002604
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002586
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001096
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :001096
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001096
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004997
Links to Exploration step
Pascal:05-0367924Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng</s1>
<s2>Singapore 3084332</s2>
<s3>SGP</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 3084332</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee Kok Keong" sort="Lee Kok Keong" uniqKey="Lee Kok Keong" last="Lee Kok Keong">LEE KOK KEONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lim Seng Gee" sort="Lim Seng Gee" uniqKey="Lim Seng Gee" last="Lim Seng Gee">LIM SENG GEE</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hong Wanjin" sort="Hong Wanjin" uniqKey="Hong Wanjin" last="Hong Wanjin">HONG WANJIN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0367924</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0367924 INIST</idno>
<idno type="RBID">Pascal:05-0367924</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000626</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000364</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000600</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000600</idno>
<idno type="wicri:doubleKey">0095-1137:2005:Inoue M:performance:of:single</idno>
<idno type="wicri:Area/Main/Merge">005120</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:16081995</idno>
<idno type="wicri:Area/PubMed/Corpus">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002604</idno>
<idno type="wicri:Area/PubMed/Curation">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002604</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002586</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002586</idno>
<idno type="wicri:Area/Ncbi/Merge">001096</idno>
<idno type="wicri:Area/Ncbi/Curation">001096</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001096</idno>
<idno type="wicri:doubleKey">0095-1137:2005:Inoue M:performance:of:single</idno>
<idno type="wicri:Area/Main/Merge">004997</idno>
<idno type="wicri:Area/Main/Curation">004D83</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng</s1>
<s2>Singapore 3084332</s2>
<s3>SGP</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 3084332</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee Kok Keong" sort="Lee Kok Keong" uniqKey="Lee Kok Keong" last="Lee Kok Keong">LEE KOK KEONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lim Seng Gee" sort="Lim Seng Gee" uniqKey="Lim Seng Gee" last="Lim Seng Gee">LIM SENG GEE</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hong Wanjin" sort="Hong Wanjin" uniqKey="Hong Wanjin" last="Hong Wanjin">HONG WANJIN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Detection</term>
<term>Humans</term>
<term>Microbiology</term>
<term>Real time</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Humains</term>
<term>RT-PCR</term>
<term>Réaction chaîne polymérase RT</term>
<term>Sensibilité et spécificité</term>
<term>Temps réel</term>
<term>Détection</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.</div>
</front>
</TEI>
<double idat="0095-1137:2005:Inoue M:performance:of:single"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng</s1>
<s2>Singapore 3084332</s2>
<s3>SGP</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 3084332</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee Kok Keong" sort="Lee Kok Keong" uniqKey="Lee Kok Keong" last="Lee Kok Keong">LEE KOK KEONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lim Seng Gee" sort="Lim Seng Gee" uniqKey="Lim Seng Gee" last="Lim Seng Gee">LIM SENG GEE</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hong Wanjin" sort="Hong Wanjin" uniqKey="Hong Wanjin" last="Hong Wanjin">HONG WANJIN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0367924</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0367924 INIST</idno>
<idno type="RBID">Pascal:05-0367924</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000626</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000364</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000600</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000600</idno>
<idno type="wicri:doubleKey">0095-1137:2005:Inoue M:performance:of:single</idno>
<idno type="wicri:Area/Main/Merge">005120</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pathology and Laboratory Medicine, Tan Took Seng Hospital, 11 Jalan Tan Took Seng</s1>
<s2>Singapore 3084332</s2>
<s3>SGP</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 3084332</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee Kok Keong" sort="Lee Kok Keong" uniqKey="Lee Kok Keong" last="Lee Kok Keong">LEE KOK KEONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lim Seng Gee" sort="Lim Seng Gee" uniqKey="Lim Seng Gee" last="Lim Seng Gee">LIM SENG GEE</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hong Wanjin" sort="Hong Wanjin" uniqKey="Hong Wanjin" last="Hong Wanjin">HONG WANJIN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore 138673</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Detection</term>
<term>Microbiology</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Réaction chaîne polymérase RT</term>
<term>Temps réel</term>
<term>Détection</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.</div>
</front>
</TEI>
</INIST>
<PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
</author>
<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
</author>
<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
</author>
<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16081995</idno>
<idno type="pmid">16081995</idno>
<idno type="doi">10.1128/JCM.43.8.4262-4265.2005</idno>
<idno type="wicri:Area/PubMed/Corpus">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002604</idno>
<idno type="wicri:Area/PubMed/Curation">002604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002604</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002586</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002586</idno>
<idno type="wicri:Area/Ncbi/Merge">001096</idno>
<idno type="wicri:Area/Ncbi/Curation">001096</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001096</idno>
<idno type="wicri:doubleKey">0095-1137:2005:Inoue M:performance:of:single</idno>
<idno type="wicri:Area/Main/Merge">004997</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.</title>
<author><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
</author>
<author><name sortKey="Keong, Lee Kok" sort="Keong, Lee Kok" uniqKey="Keong L" first="Lee Kok" last="Keong">Lee Kok Keong</name>
</author>
<author><name sortKey="Gee, Lim Seng" sort="Gee, Lim Seng" uniqKey="Gee L" first="Lim Seng" last="Gee">Lim Seng Gee</name>
</author>
<author><name sortKey="Wanjin, Hong" sort="Wanjin, Hong" uniqKey="Wanjin H" first="Hong" last="Wanjin">Hong Wanjin</name>
</author>
</analytic>
<series><title level="j">Journal of clinical microbiology</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Humains</term>
<term>RT-PCR</term>
<term>Sensibilité et spécificité</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.</div>
</front>
</TEI>
</PubMed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004D83 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004D83 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Curation |type= RBID |clé= Pascal:05-0367924 |texte= Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus }}
This area was generated with Dilib version V0.6.33. |