Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Identifieur interne : 005120 ( Main/Merge ); précédent : 005119; suivant : 005121Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus
Auteurs : Masafumi Inoue [Singapour] ; Timothy Barkham [Singapour] ; LEE KOK KEONG [Singapour] ; LIM SENG GEE [Singapour] ; HONG WANJIN [Singapour]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.
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Pascal:05-0367924Le document en format XML
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<term>Réaction chaîne polymérase RT</term>
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<front><div type="abstract" xml:lang="en">Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 μl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 ± 46 GE.</div>
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<affiliations><list><country><li>Singapour</li>
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<tree><country name="Singapour"><noRegion><name sortKey="Inoue, Masafumi" sort="Inoue, Masafumi" uniqKey="Inoue M" first="Masafumi" last="Inoue">Masafumi Inoue</name>
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<name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name>
<name sortKey="Hong Wanjin" sort="Hong Wanjin" uniqKey="Hong Wanjin" last="Hong Wanjin">HONG WANJIN</name>
<name sortKey="Lee Kok Keong" sort="Lee Kok Keong" uniqKey="Lee Kok Keong" last="Lee Kok Keong">LEE KOK KEONG</name>
<name sortKey="Lim Seng Gee" sort="Lim Seng Gee" uniqKey="Lim Seng Gee" last="Lim Seng Gee">LIM SENG GEE</name>
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