LrgpV1, Main, Exploration, bibRecordById, pubmed:23442668

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Identifieur interne : 000924 ( Main/Exploration ); précédent : 000923; suivant : 000925

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Auteurs : Claudia Nioi [France] ; Romain Kapel [France] ; Emmanuel Rondags [France] ; Ivan Marc [France]

Source :

Food chemistry [ 0308-8146 ] ; 2012.

RBID : Pascal:12-0258291

Descripteurs français

KwdFr :
- Antifongiques (), Antifongiques (isolement et purification), Antifongiques (pharmacologie), Brassica rapa (), Chromatographie sur gel, Extraits de plantes (), Extraits de plantes (isolement et purification), Extraits de plantes (pharmacologie), Fusarium ().
MESH :
- isolement et purification : Antifongiques, Extraits de plantes.
- pharmacologie : Antifongiques, Extraits de plantes.
Pascal (Inist)
- Antifongiques, Brassica rapa, Chromatographie sur gel, Extraction, Antifongique, Extraits de plantes, Fusarium, Tourteau colza, Farine colza, Protéine, Procédé extraction, Sélectivité, Propriété, Fusarium.

English descriptors

KwdEn :
- Antifungal Agents (chemistry), Antifungal Agents (isolation & purification), Antifungal Agents (pharmacology), Antifungal agent, Brassica rapa (chemistry), Chromatography, Gel, Extraction, Extraction process, Fusarium, Fusarium (drug effects), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Extracts (pharmacology), Properties, Protein, Rapeseed cake, Rapeseed meal, Selectivity.
MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
mix :
- Antifungal properties, BRASSICA-JUNCEA, CALMODULIN ANTAGONISTS, CANOLA PROTEINS, DEPENDENT PROTEIN-KINASE, FUNCTIONAL-PROPERTIES, Fusarium langsethiae, HYDROLYSIS, LARGE CHAINS, Napins, PURIFICATION, Protein extraction process, Rapeseed meal, STABILITY, SUBUNIT, Selectivity.

Abstract

This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC₅₀ = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.

Url:

https://hal.archives-ouvertes.fr/hal-00777695

Affiliations:

Le document en format XML

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<term>Antifungal Agents (isolation & purification)</term>
<term>Antifungal Agents (pharmacology)</term>
<term>Antifungal agent</term>
<term>Brassica rapa (chemistry)</term>
<term>Chromatography, Gel</term>
<term>Extraction</term>
<term>Extraction process</term>
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<term>Plant Extracts (isolation & purification)</term>
<term>Plant Extracts (pharmacology)</term>
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<term>Rapeseed meal</term>
<term>Selectivity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques ()</term>
<term>Antifongiques (isolement et purification)</term>
<term>Antifongiques (pharmacologie)</term>
<term>Brassica rapa ()</term>
<term>Chromatographie sur gel</term>
<term>Extraits de plantes ()</term>
<term>Extraits de plantes (isolement et purification)</term>
<term>Extraits de plantes (pharmacologie)</term>
<term>Fusarium ()</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term>
<term>Plant Extracts</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antifungal Agents</term>
<term>Plant Extracts</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term>
<term>Plant Extracts</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Brassica rapa</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fusarium</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term>
<term>Extraits de plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term>
<term>Extraits de plantes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Chromatography, Gel</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Antifongiques</term>
<term>Brassica rapa</term>
<term>Chromatographie sur gel</term>
<term>Extraction</term>
<term>Antifongique</term>
<term>Extraits de plantes</term>
<term>Fusarium</term>
<term>Tourteau colza</term>
<term>Farine colza</term>
<term>Protéine</term>
<term>Procédé extraction</term>
<term>Sélectivité</term>
<term>Propriété</term>
<term>Fusarium</term>
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<keywords scheme="mix" xml:lang="en"><term>Antifungal properties</term>
<term>BRASSICA-JUNCEA</term>
<term>CALMODULIN ANTAGONISTS</term>
<term>CANOLA PROTEINS</term>
<term>DEPENDENT PROTEIN-KINASE</term>
<term>FUNCTIONAL-PROPERTIES</term>
<term>Fusarium langsethiae</term>
<term>HYDROLYSIS</term>
<term>LARGE CHAINS</term>
<term>Napins</term>
<term>PURIFICATION</term>
<term>Protein extraction process</term>
<term>Rapeseed meal</term>
<term>STABILITY</term>
<term>SUBUNIT</term>
<term>Selectivity</term>
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC<sub>50</sub>
 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
</front>
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</country>
<region><li>Grand Est</li>
<li>Lorraine (région)</li>
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<tree><country name="France"><region name="Grand Est"><name sortKey="Nioi, Claudia" sort="Nioi, Claudia" uniqKey="Nioi C" first="Claudia" last="Nioi">Claudia Nioi</name>
</region>
<name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name>
<name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
<name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name>
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Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Source :

Descripteurs français

English descriptors

Abstract

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