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Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Identifieur interne : 000974 ( PascalFrancis/Curation ); précédent : 000973; suivant : 000975

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Auteurs : Claudia Nioi [France] ; Romain Kapel [France] ; Emmanuel Rondags [France] ; Ivan Marc [France]

Source :

RBID : Pascal:12-0258291

Descripteurs français

English descriptors

Abstract

This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC50 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
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A11 01  1    @1 NIOI (Claudia)
A11 02  1    @1 KAPEL (Romain)
A11 03  1    @1 RONDAGS (Emmanuel)
A11 04  1    @1 MARC (Ivan)
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C01 01    ENG  @0 This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC50 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
C02 01  X    @0 002A35A03
C03 01  X  FRE  @0 Extraction @5 01
C03 01  X  ENG  @0 Extraction @5 01
C03 01  X  SPA  @0 Extracción @5 01
C03 02  X  FRE  @0 Antifongique @5 02
C03 02  X  ENG  @0 Antifungal agent @5 02
C03 02  X  SPA  @0 Antifúngico @5 02
C03 03  X  FRE  @0 Tourteau colza @5 10
C03 03  X  ENG  @0 Rapeseed cake @5 10
C03 03  X  SPA  @0 Torta colza @5 10
C03 04  X  FRE  @0 Farine colza @5 19
C03 04  X  ENG  @0 Rapeseed meal @5 19
C03 04  X  SPA  @0 Harina colza @5 19
C03 05  X  FRE  @0 Protéine @5 20
C03 05  X  ENG  @0 Protein @5 20
C03 05  X  SPA  @0 Proteína @5 20
C03 06  X  FRE  @0 Procédé extraction @5 24
C03 06  X  ENG  @0 Extraction process @5 24
C03 06  X  SPA  @0 Procedimiento extracción @5 24
C03 07  X  FRE  @0 Sélectivité @5 26
C03 07  X  ENG  @0 Selectivity @5 26
C03 07  X  SPA  @0 Selectividad @5 26
C03 08  X  FRE  @0 Propriété @5 48
C03 08  X  ENG  @0 Properties @5 48
C03 08  X  SPA  @0 Propiedad @5 48
C03 09  X  FRE  @0 Fusarium @2 NS @5 53
C03 09  X  ENG  @0 Fusarium @2 NS @5 53
C03 09  X  SPA  @0 Fusarium @2 NS @5 53
C07 01  X  FRE  @0 Fungi Imperfecti @2 NS
C07 01  X  ENG  @0 Fungi Imperfecti @2 NS
C07 01  X  SPA  @0 Fungi Imperfecti @2 NS
C07 02  X  FRE  @0 Fungi @2 NS
C07 02  X  ENG  @0 Fungi @2 NS
C07 02  X  SPA  @0 Fungi @2 NS
N21       @1 198
N44 01      @1 OTO
N82       @1 OTO

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Pascal:12-0258291

Le document en format XML

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