LrgpV1, PascalFrancis, Checkpoint, bibRecord, 000077

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Identifieur interne : 000077 ( PascalFrancis/Checkpoint ); précédent : 000076; suivant : 000078

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Auteurs : Claudia Nioi [France] ; Romain Kapel [France] ; Emmanuel Rondags [France] ; Ivan Marc [France]

Source :

Food chemistry [ 0308-8146 ] ; 2012.

RBID : Pascal:12-0258291

Descripteurs français

Pascal (Inist)
- Extraction, Antifongique, Tourteau colza, Farine colza, Protéine, Procédé extraction, Sélectivité, Propriété, Fusarium.

English descriptors

KwdEn :
- Antifungal agent, Extraction, Extraction process, Fusarium, Properties, Protein, Rapeseed cake, Rapeseed meal, Selectivity.

Abstract

This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC₅₀ = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.

Affiliations:

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Pascal:12-0258291

Le document en format XML

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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC<sub>50</sub>
 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

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Descripteurs français

English descriptors

Abstract

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