Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal
Identifieur interne : 000077 ( PascalFrancis/Checkpoint ); précédent : 000076; suivant : 000078Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal
Auteurs : Claudia Nioi [France] ; Romain Kapel [France] ; Emmanuel Rondags [France] ; Ivan Marc [France]Source :
- Food chemistry [ 0308-8146 ] ; 2012.
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- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC50 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
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