Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Identifieur interne : 000061 ( PubMed/Corpus ); précédent : 000060; suivant : 000062Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Auteurs : Claudia Nioi ; Romain Kapel ; Emmanuel Rondags ; Ivan MarcSource :
- Food chemistry [ 0308-8146 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
Abstract
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
DOI: 10.1016/j.foodchem.2012.04.017
PubMed: 23442668
Links to Exploration step
pubmed:23442668Le document en format XML
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<author><name sortKey="Nioi, Claudia" sort="Nioi, Claudia" uniqKey="Nioi C" first="Claudia" last="Nioi">Claudia Nioi</name>
<affiliation><nlm:affiliation>LRGP, UPR CNRS 3349, Lorraine University, Plateforme SVS, 13 rue du bois de la Champelle, 54500 Vandœuvre-lès-Nancy, France.</nlm:affiliation>
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<author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name>
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<author><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name>
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<author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
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<author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name>
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<author><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name>
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<term>Brassica rapa (chemistry)</term>
<term>Chromatography, Gel</term>
<term>Fusarium (drug effects)</term>
<term>Plant Extracts (chemistry)</term>
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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<Abstract><AbstractText>This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</AbstractText>
<CopyrightInformation>Copyright © 2012 Elsevier Ltd. All rights reserved.</CopyrightInformation>
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