Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Identifieur interne : 000261 ( Ncbi/Checkpoint ); précédent : 000260; suivant : 000262Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Auteurs : Claudia Nioi [France] ; Romain Kapel ; Emmanuel Rondags ; Ivan MarcSource :
- Food chemistry [ 0308-8146 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antifongiques, Extraits de plantes.
- pharmacologie : Antifongiques, Extraits de plantes.
- Antifongiques, Brassica rapa, Chromatographie sur gel, Extraits de plantes, Fusarium.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
Abstract
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
DOI: 10.1016/j.foodchem.2012.04.017
PubMed: 23442668
Affiliations:
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pubmed:23442668Le document en format XML
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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