Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal
Identifieur interne : 000924 ( Main/Curation ); précédent : 000923; suivant : 000925Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal
Auteurs : Claudia Nioi [France] ; Romain Kapel [France] ; Emmanuel Rondags [France] ; Ivan Marc [France]Source :
- Food chemistry [ 0308-8146 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antifongiques, Extraits de plantes.
- pharmacologie : Antifongiques, Extraits de plantes.
- Pascal (Inist)
English descriptors
- KwdEn :
- Antifungal Agents (chemistry), Antifungal Agents (isolation & purification), Antifungal Agents (pharmacology), Antifungal agent, Brassica rapa (chemistry), Chromatography, Gel, Extraction, Extraction process, Fusarium, Fusarium (drug effects), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Extracts (pharmacology), Properties, Protein, Rapeseed cake, Rapeseed meal, Selectivity.
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
- mix :
Abstract
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC50 = 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
Url:
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Pascal:12-0258291Le document en format XML
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<term>Antifungal Agents (pharmacology)</term>
<term>Antifungal agent</term>
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<term>Chromatography, Gel</term>
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<term>Extraction process</term>
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<term>Plant Extracts (chemistry)</term>
<term>Plant Extracts (isolation & purification)</term>
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<term>Selectivity</term>
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<term>Antifongiques (isolement et purification)</term>
<term>Antifongiques (pharmacologie)</term>
<term>Brassica rapa ()</term>
<term>Chromatographie sur gel</term>
<term>Extraits de plantes ()</term>
<term>Extraits de plantes (isolement et purification)</term>
<term>Extraits de plantes (pharmacologie)</term>
<term>Fusarium ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term>
<term>Plant Extracts</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antifungal Agents</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fusarium</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term>
<term>Extraits de plantes</term>
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<term>Extraits de plantes</term>
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<term>Brassica rapa</term>
<term>Chromatographie sur gel</term>
<term>Extraction</term>
<term>Antifongique</term>
<term>Extraits de plantes</term>
<term>Fusarium</term>
<term>Tourteau colza</term>
<term>Farine colza</term>
<term>Protéine</term>
<term>Procédé extraction</term>
<term>Sélectivité</term>
<term>Propriété</term>
<term>Fusarium</term>
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<term>CALMODULIN ANTAGONISTS</term>
<term>CANOLA PROTEINS</term>
<term>DEPENDENT PROTEIN-KINASE</term>
<term>FUNCTIONAL-PROPERTIES</term>
<term>Fusarium langsethiae</term>
<term>HYDROLYSIS</term>
<term>LARGE CHAINS</term>
<term>Napins</term>
<term>PURIFICATION</term>
<term>Protein extraction process</term>
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC<sub>50</sub>
= 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
</front>
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<author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
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<s3>FRA</s3>
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<series><title level="j" type="main">Food chemistry</title>
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<term>Extraction process</term>
<term>Fusarium</term>
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<term>Protein</term>
<term>Rapeseed cake</term>
<term>Rapeseed meal</term>
<term>Selectivity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Extraction</term>
<term>Antifongique</term>
<term>Tourteau colza</term>
<term>Farine colza</term>
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<term>Procédé extraction</term>
<term>Sélectivité</term>
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC<sub>50</sub>
= 70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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<term>LARGE CHAINS</term>
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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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