Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Identifieur interne : 004120 ( Main/Exploration ); précédent : 004119; suivant : 004121Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Auteurs : Hongwei Shao [République populaire de Chine] ; Dongming Lan [République populaire de Chine] ; Zhaohui Duan [République populaire de Chine] ; Zehuan Liu [République populaire de Chine] ; Jun Min [République populaire de Chine] ; Lichun Zhang [République populaire de Chine] ; Jian Huang [République populaire de Chine] ; Jing Su [République populaire de Chine] ; Shangwu Chen [République populaire de Chine] ; Anlong Xu [République populaire de Chine]Source :
- Journal of Clinical Immunology [ 0271-9142 ] ; 2006-11-01.
Descripteurs français
- KwdFr :
- ADN mitochondrial (analyse), Adulte, Agranulocytes (métabolisme), Agranulocytes (virologie), Analyse de profil d'expression de gènes, Femelle, Gènes de mitochondrie, Humains, Hybridation d'acides nucléiques, Microscopie électronique, Microscopie électronique à transmission, RT-PCR, Régulation positive, Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Techniques in vitro, Virus du SRAS (métabolisme).
- MESH :
- analyse : ADN mitochondrial.
- métabolisme : Agranulocytes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- virologie : Agranulocytes, Syndrome respiratoire aigu sévère.
- Adulte, Analyse de profil d'expression de gènes, Femelle, Gènes de mitochondrie, Humains, Hybridation d'acides nucléiques, Microscopie électronique, Microscopie électronique à transmission, RT-PCR, Régulation positive, Techniques in vitro.
English descriptors
- KwdEn :
- Adult, DNA, Mitochondrial (analysis), Female, Gene Expression Profiling, Genes, Mitochondrial, Humans, In Vitro Techniques, Leukocytes, Mononuclear (metabolism), Leukocytes, Mononuclear (virology), Microscopy, Electron, Microscopy, Electron, Transmission, Nucleic Acid Hybridization, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (metabolism), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Up-Regulation, mitochondria, peripheral blood mononuclear cell, suppression subtractive hybridization.
- MESH :
- chemical , analysis : DNA, Mitochondrial.
- metabolism : Leukocytes, Mononuclear, SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Leukocytes, Mononuclear, Severe Acute Respiratory Syndrome.
- Adult, Female, Gene Expression Profiling, Genes, Mitochondrial, Humans, In Vitro Techniques, Microscopy, Electron, Microscopy, Electron, Transmission, Nucleic Acid Hybridization, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation.
Abstract
The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.
Url:
- https://api.istex.fr/ark:/67375/VQC-WF136QD8-4/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086694
DOI: 10.1007/s10875-006-9046-y
Affiliations:
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Le document en format XML
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<term>DNA, Mitochondrial (analysis)</term>
<term>Female</term>
<term>Gene Expression Profiling</term>
<term>Genes, Mitochondrial</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Leukocytes, Mononuclear (metabolism)</term>
<term>Leukocytes, Mononuclear (virology)</term>
<term>Microscopy, Electron</term>
<term>Microscopy, Electron, Transmission</term>
<term>Nucleic Acid Hybridization</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Severe acute respiratory syndrome</term>
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<term>suppression subtractive hybridization</term>
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<term>Agranulocytes (métabolisme)</term>
<term>Agranulocytes (virologie)</term>
<term>Analyse de profil d'expression de gènes</term>
<term>Femelle</term>
<term>Gènes de mitochondrie</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Microscopie électronique</term>
<term>Microscopie électronique à transmission</term>
<term>RT-PCR</term>
<term>Régulation positive</term>
<term>Syndrome respiratoire aigu sévère (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
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<term>Gene Expression Profiling</term>
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<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Microscopy, Electron</term>
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<term>Nucleic Acid Hybridization</term>
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<term>Analyse de profil d'expression de gènes</term>
<term>Femelle</term>
<term>Gènes de mitochondrie</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Microscopie électronique</term>
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<front><div type="abstract" xml:lang="en">The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Chen, Shangwu" sort="Chen, Shangwu" uniqKey="Chen S" first="Shangwu" last="Chen">Shangwu Chen</name>
<name sortKey="Duan, Zhaohui" sort="Duan, Zhaohui" uniqKey="Duan Z" first="Zhaohui" last="Duan">Zhaohui Duan</name>
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<name sortKey="Liu, Zehuan" sort="Liu, Zehuan" uniqKey="Liu Z" first="Zehuan" last="Liu">Zehuan Liu</name>
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<name sortKey="Su, Jing" sort="Su, Jing" uniqKey="Su J" first="Jing" last="Su">Jing Su</name>
<name sortKey="Xu, Anlong" sort="Xu, Anlong" uniqKey="Xu A" first="Anlong" last="Xu">Anlong Xu</name>
<name sortKey="Xu, Anlong" sort="Xu, Anlong" uniqKey="Xu A" first="Anlong" last="Xu">Anlong Xu</name>
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