Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Identifieur interne : 001546 ( Istex/Checkpoint ); précédent : 001545; suivant : 001547Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Auteurs : Hongwei Shao [République populaire de Chine] ; Dongming Lan [République populaire de Chine] ; Zhaohui Duan [République populaire de Chine] ; Zehuan Liu [République populaire de Chine] ; Jun Min [République populaire de Chine] ; Lichun Zhang [République populaire de Chine] ; Jian Huang [République populaire de Chine] ; Jing Su [République populaire de Chine] ; Shangwu Chen [République populaire de Chine] ; Anlong Xu [République populaire de Chine]Source :
- Journal of Clinical Immunology [ 0271-9142 ] ; 2006-11-01.
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Abstract
The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.
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DOI: 10.1007/s10875-006-9046-y
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In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Shao, Hongwei" sort="Shao, Hongwei" uniqKey="Shao H" first="Hongwei" last="Shao">Hongwei Shao</name></region><name sortKey="Chen, Shangwu" sort="Chen, Shangwu" uniqKey="Chen S" first="Shangwu" last="Chen">Shangwu Chen</name><name sortKey="Duan, Zhaohui" sort="Duan, Zhaohui" uniqKey="Duan Z" first="Zhaohui" last="Duan">Zhaohui Duan</name><name sortKey="Huang, Jian" sort="Huang, Jian" uniqKey="Huang J" first="Jian" last="Huang">Jian Huang</name><name sortKey="Lan, Dongming" sort="Lan, Dongming" uniqKey="Lan D" first="Dongming" last="Lan">Dongming Lan</name><name sortKey="Liu, Zehuan" sort="Liu, Zehuan" uniqKey="Liu Z" first="Zehuan" last="Liu">Zehuan Liu</name><name sortKey="Min, Jun" sort="Min, Jun" uniqKey="Min J" first="Jun" last="Min">Jun Min</name><name sortKey="Su, Jing" sort="Su, Jing" uniqKey="Su J" first="Jing" last="Su">Jing Su</name><name sortKey="Xu, Anlong" sort="Xu, Anlong" uniqKey="Xu A" first="Anlong" last="Xu">Anlong Xu</name><name sortKey="Xu, Anlong" sort="Xu, Anlong" uniqKey="Xu A" first="Anlong" last="Xu">Anlong Xu</name><name sortKey="Zhang, Lichun" sort="Zhang, Lichun" uniqKey="Zhang L" first="Lichun" last="Zhang">Lichun Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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