Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Identifieur interne : 000138 ( Istex/Corpus ); précédent : 000137; suivant : 000139Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients
Auteurs : Hongwei Shao ; Dongming Lan ; Zhaohui Duan ; Zehuan Liu ; Jun Min ; Lichun Zhang ; Jian Huang ; Jing Su ; Shangwu Chen ; Anlong XuSource :
- Journal of Clinical Immunology [ 0271-9142 ] ; 2006-11-01.
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Abstract
The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.
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DOI: 10.1007/s10875-006-9046-y
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R. China</mods:affiliation></affiliation></author><author><name sortKey="Lan, Dongming" sort="Lan, Dongming" uniqKey="Lan D" first="Dongming" last="Lan">Dongming Lan</name><affiliation><mods:affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Duan, Zhaohui" sort="Duan, Zhaohui" uniqKey="Duan Z" first="Zhaohui" last="Duan">Zhaohui Duan</name><affiliation><mods:affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Zehuan" sort="Liu, Zehuan" uniqKey="Liu Z" first="Zehuan" last="Liu">Zehuan Liu</name><affiliation><mods:affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Min, Jun" sort="Min, Jun" uniqKey="Min J" first="Jun" last="Min">Jun Min</name><affiliation><mods:affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Lichun" sort="Zhang, Lichun" uniqKey="Zhang L" first="Lichun" last="Zhang">Lichun Zhang</name><affiliation><mods:affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Huang, Jian" sort="Huang, Jian" uniqKey="Huang J" first="Jian" last="Huang">Jian Huang</name><affiliation><mods:affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. 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In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>HONGWEI SHAO</name><affiliations><json:string>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</json:string></affiliations></json:item><json:item><name>DONGMING LAN</name><affiliations><json:string>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</json:string></affiliations></json:item><json:item><name>ZHAOHUI DUAN</name><affiliations><json:string>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. 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In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. 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In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. 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TocLevels="0"><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd><IssueArticleCount>6</IssueArticleCount><IssueHistory><OnlineDate><Year>2006</Year><Month>11</Month><Day>15</Day></OnlineDate><PrintDate><Year>2006</Year><Month>11</Month><Day>15</Day></PrintDate><CoverDate><Year>2006</Year><Month>11</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2006</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s10875-006-9046-y" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9046</ArticleID><ArticleDOI>10.1007/s10875-006-9046-y</ArticleDOI><ArticleSequenceNumber>6</ArticleSequenceNumber><ArticleTitle Language="En">Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients</ArticleTitle><ArticleFirstPage>546</ArticleFirstPage><ArticleLastPage>554</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2006</Year><Month>9</Month><Day>8</Day></RegistrationDate><Received><Year>2006</Year><Month>5</Month><Day>30</Day></Received><Accepted><Year>2006</Year><Month>9</Month><Day>8</Day></Accepted><OnlineDate><Year>2006</Year><Month>10</Month><Day>6</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2006</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><PageHeaders><RunningTitle>Gene Profile in Recovered SARS Patients</RunningTitle><RunningAuthor>shao et al.</RunningAuthor></PageHeaders><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>HONGWEI</GivenName><FamilyName>SHAO</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>DONGMING</GivenName><FamilyName>LAN</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>ZHAOHUI</GivenName><FamilyName>DUAN</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>ZEHUAN</GivenName><FamilyName>LIU</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>JUN</GivenName><FamilyName>MIN</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>LICHUN</GivenName><FamilyName>ZHANG</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>JIAN</GivenName><FamilyName>HUANG</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>JING</GivenName><FamilyName>SU</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>SHANGWU</GivenName><FamilyName>CHEN</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>ANLONG</GivenName><FamilyName>XU</FamilyName></AuthorName><Contact><Email>lssxal@mail.sysu.edu.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>State Key Laboratory of Biocontrol, Department of Biochemistry</OrgDivision><OrgName>College of Life Sciences, Sun Yat-sen (Zhongshan) University</OrgName><OrgAddress><Postcode>510275</Postcode><City>Guangzhou</City><Country Code="CN">P. R. China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University</OrgName><OrgAddress><Postcode>510120</Postcode><City>Guangzhou</City><Country Code="CN">P. R. China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Para>The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV <Emphasis Type="Italic">in vivo</Emphasis>. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.</Para></Abstract><KeywordGroup Language="En"><Heading>KEY WORDS</Heading><Keyword>Severe acute respiratory syndrome</Keyword><Keyword>peripheral blood mononuclear cell</Keyword><Keyword>suppression subtractive hybridization</Keyword><Keyword>mitochondria</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>Both authors contributed equally to the work, China</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Upregulation of Mitochondrial Gene Expression in PBMC from Convalescent SARS Patients</title></titleInfo><name type="personal"><namePart type="given">HONGWEI</namePart><namePart type="family">SHAO</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DONGMING</namePart><namePart type="family">LAN</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ZHAOHUI</namePart><namePart type="family">DUAN</namePart><affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ZEHUAN</namePart><namePart type="family">LIU</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JUN</namePart><namePart type="family">MIN</namePart><affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LICHUN</namePart><namePart type="family">ZHANG</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JIAN</namePart><namePart type="family">HUANG</namePart><affiliation>The Second Affiliated Hospital, Sun Yat-sen (Zhongshan) University, 510120, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JING</namePart><namePart type="family">SU</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SHANGWU</namePart><namePart type="family">CHEN</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">ANLONG</namePart><namePart type="family">XU</namePart><affiliation>State Key Laboratory of Biocontrol, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 510275, Guangzhou, P. R. China</affiliation><affiliation>E-mail: lssxal@mail.sysu.edu.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com</publisher><place><placeTerm type="text">New York</placeTerm></place><dateCreated encoding="w3cdtf">2006-05-30</dateCreated><dateIssued encoding="w3cdtf">2006-11-01</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.</abstract><subject lang="en"><genre>KEY WORDS</genre><topic>Severe acute respiratory syndrome</topic><topic>peripheral blood mononuclear cell</topic><topic>suppression subtractive hybridization</topic><topic>mitochondria</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Clinical Immunology</title></titleInfo><titleInfo type="abbreviated"><title>J Clin Immunol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2006-11-15</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Internal Medicine</topic><topic>Infectious Diseases</topic><topic>Immunology</topic></subject><identifier type="ISSN">0271-9142</identifier><identifier type="eISSN">1573-2592</identifier><identifier type="JournalID">10875</identifier><identifier type="IssueArticleCount">6</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2006</date><detail type="volume"><number>26</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>546</start><end>554</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media, LLC, 2006</recordOrigin></recordInfo></relatedItem><identifier type="istex">4B61867A3C9485A4AABECD407EB1D8B493B12D4C</identifier><identifier type="ark">ark:/67375/VQC-WF136QD8-4</identifier><identifier type="DOI">10.1007/s10875-006-9046-y</identifier><identifier type="ArticleID">9046</identifier><identifier type="ArticleID">s10875-006-9046-y</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media, LLC, 2006</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media, LLC, 2006</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-WF136QD8-4/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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