SrasV1, Main, Curation, bibRecord, 005A57

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development

Identifieur interne : 005A57 ( Main/Curation ); précédent : 005A56; suivant : 005A58

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development

Auteurs : Wai-Yan Choy [Hong Kong] ; Shu-Guang Lin [République populaire de Chine] ; Paul Kay-Sheung Chan [Hong Kong] ; John Siu-Lun Tam [Hong Kong] ; Y. M. Dennis Lo [Hong Kong] ; Ida Miu-Ting Chu [Hong Kong] ; Sau-Na Tsai [Hong Kong] ; Ming-Qi Zhong [Hong Kong] ; Kwok-Pui Fung [Hong Kong] ; Mary Miu-Yee Waye [Hong Kong] ; Stephen Kwok-Wing Tsui [Hong Kong] ; Kai-On Ng [Hong Kong] ; Zhi-Xin Shan [République populaire de Chine] ; MIN YANG [République populaire de Chine] ; Yi-Long Wu [République populaire de Chine] ; Zhan-Yi Lin [République populaire de Chine] ; Sai-Ming Ngai [Hong Kong]

Source :

Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2004.

RBID : Pascal:04-0547697

Descripteurs français

KwdFr :
- Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Haplorhini, Lapins, Microscopie confocale, Microscopie de fluorescence, Peptides (), Peptides (immunologie), Peptides (synthèse chimique), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Séquence d'acides aminés, Test ELISA, Vaccination, Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
MESH :
- immunologie : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Vaccins antiviraux, Virus du SRAS.
- synthèse chimique : Peptides.
Pascal (Inist)
- Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Haplorhini, Lapins, Microscopie confocale, Microscopie de fluorescence, Peptides, Produit synthétique, Peptide, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Coronavirus, Glycoprotéine, Séquence d'acides aminés, Test ELISA, Vaccin, Immunoprophylaxie, Prévention, Développement, Biochimie, Biologie clinique, Biologie moléculaire, Vaccination.
Wicri :
- topic : Vaccin, Biochimie.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Biochemistry, Clinical biology, Coronavirus, Development, Enzyme-Linked Immunosorbent Assay, Glycoprotein, Haplorhini, Immunoprophylaxis, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Molecular biology, Peptides, Peptides (chemical synthesis), Peptides (chemistry), Peptides (immunology), Prevention, Rabbits, SARS Virus (immunology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Synthetic product, Vaccination, Vaccine, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
MESH :
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Peptides, Viral Envelope Proteins, Viral Vaccines.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Haplorhini, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Rabbits, Spike Glycoprotein, Coronavirus, Vaccination.

Abstract

Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.

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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development</title>
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<author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name>
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<author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
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<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Biochemistry</term>
<term>Clinical biology</term>
<term>Coronavirus</term>
<term>Development</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Glycoprotein</term>
<term>Haplorhini</term>
<term>Immunoprophylaxis</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Molecular biology</term>
<term>Peptides</term>
<term>Peptides (chemical synthesis)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (immunology)</term>
<term>Prevention</term>
<term>Rabbits</term>
<term>SARS Virus (immunology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Synthetic product</term>
<term>Vaccination</term>
<term>Vaccine</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides ()</term>
<term>Peptides (immunologie)</term>
<term>Peptides (synthèse chimique)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccination</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antiviraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Haplorhini</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Rabbits</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides</term>
<term>Produit synthétique</term>
<term>Peptide</term>
<term>Protéines de l'enveloppe virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Coronavirus</term>
<term>Glycoprotéine</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccin</term>
<term>Immunoprophylaxie</term>
<term>Prévention</term>
<term>Développement</term>
<term>Biochimie</term>
<term>Biologie clinique</term>
<term>Biologie moléculaire</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
<term>Biochimie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.</div>
</front>
</TEI>
<double idat="0009-9147:2004:Choy W:synthetic:peptide:studies"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development</title>
<author><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y. M. Dennis" last="Lo">Y. M. Dennis Lo</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
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<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name>
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<sZ>2 aut.</sZ>
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<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
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</inist:fA14>
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</affiliation>
</author>
<author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
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</affiliation>
</author>
<author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
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<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">04-0547697</idno>
<date when="2004">2004</date>
<idno type="stanalyst">PASCAL 04-0547697 INIST</idno>
<idno type="RBID">Pascal:04-0547697</idno>
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</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development</title>
<author><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name>
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<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
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</inist:fA14>
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<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y. M. Dennis" last="Lo">Y. M. Dennis Lo</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
<author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1>
<s2>Guangdong</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1>
<s2>Shatin</s2>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
<orgName type="university">Université chinoise de Hong Kong</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title>
<title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title>
<idno type="ISSN">0009-9147</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochemistry</term>
<term>Clinical biology</term>
<term>Coronavirus</term>
<term>Development</term>
<term>Glycoprotein</term>
<term>Immunoprophylaxis</term>
<term>Molecular biology</term>
<term>Peptides</term>
<term>Prevention</term>
<term>Severe acute respiratory syndrome</term>
<term>Synthetic product</term>
<term>Vaccine</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Produit synthétique</term>
<term>Peptide</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Coronavirus</term>
<term>Glycoprotéine</term>
<term>Vaccin</term>
<term>Immunoprophylaxie</term>
<term>Prévention</term>
<term>Développement</term>
<term>Biochimie</term>
<term>Biologie clinique</term>
<term>Biologie moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
<term>Biochimie</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en">Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.</div>
</front>
</TEI>
</INIST>
<PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.</title>
<author><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name>
<affiliation wicri:level="4"><nlm:affiliation>Molecular Biotechnology Program, Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Molecular Biotechnology Program, Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong</wicri:regionArea>
<orgName type="university">Université chinoise de Hong Kong</orgName>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name>
</author>
<author><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name>
</author>
<author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name>
</author>
<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name>
</author>
<author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
</author>
<author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name>
</author>
<author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name>
</author>
<author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name>
</author>
<author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
</author>
<author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name>
</author>
<author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name>
</author>
<author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name>
</author>
<author><name sortKey="Yang, Min" sort="Yang, Min" uniqKey="Yang M" first="Min" last="Yang">Min Yang</name>
</author>
<author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name>
</author>
<author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name>
</author>
<author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name>
</author>
</titleStmt>
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<date when="2004">2004</date>
<idno type="RBID">pubmed:15044316</idno>
<idno type="pmid">15044316</idno>
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<idno type="wicri:doubleKey">0009-9147:2004:Choy W:synthetic:peptide:studies</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.</title>
<author><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name>
<affiliation wicri:level="4"><nlm:affiliation>Molecular Biotechnology Program, Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Molecular Biotechnology Program, Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong</wicri:regionArea>
<orgName type="university">Université chinoise de Hong Kong</orgName>
<placeName><settlement type="city">Sha Tin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name>
</author>
<author><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name>
</author>
<author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name>
</author>
<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name>
</author>
<author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
</author>
<author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name>
</author>
<author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name>
</author>
<author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name>
</author>
<author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
</author>
<author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name>
</author>
<author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name>
</author>
<author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name>
</author>
<author><name sortKey="Yang, Min" sort="Yang, Min" uniqKey="Yang M" first="Min" last="Yang">Min Yang</name>
</author>
<author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name>
</author>
<author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name>
</author>
<author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name>
</author>
</analytic>
<series><title level="j">Clinical chemistry</title>
<idno type="ISSN">0009-9147</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Haplorhini</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Peptides (chemical synthesis)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (immunology)</term>
<term>Rabbits</term>
<term>SARS Virus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides ()</term>
<term>Peptides (immunologie)</term>
<term>Peptides (synthèse chimique)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccination</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antiviraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Haplorhini</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Rabbits</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein.</div>
</front>
</TEI>
</PubMed>
</double>
</record>

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Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development

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