Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development
Identifieur interne : 000757 ( PascalFrancis/Checkpoint ); précédent : 000756; suivant : 000758Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development
Auteurs : Wai-Yan Choy [Hong Kong] ; Shu-Guang Lin [République populaire de Chine] ; Paul Kay-Sheung Chan [Hong Kong] ; John Siu-Lun Tam [Hong Kong] ; Y. M. Dennis Lo [Hong Kong] ; Ida Miu-Ting Chu [Hong Kong] ; Sau-Na Tsai [Hong Kong] ; Ming-Qi Zhong [Hong Kong] ; Kwok-Pui Fung [Hong Kong] ; Mary Miu-Yee Waye [Hong Kong] ; Stephen Kwok-Wing Tsui [Hong Kong] ; Kai-On Ng [Hong Kong] ; Zhi-Xin Shan [République populaire de Chine] ; MIN YANG [République populaire de Chine] ; Yi-Long Wu [République populaire de Chine] ; Zhan-Yi Lin [République populaire de Chine] ; Sai-Ming Ngai [Hong Kong]Source :
- Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2004.
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Abstract
Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.
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M. Dennis" last="Lo">Y. M. Dennis Lo</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>5 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" 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Kong</orgName></affiliation></author><author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha 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Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>7 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0547697</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0547697 INIST</idno><idno type="RBID">Pascal:04-0547697</idno><idno type="wicri:Area/PascalFrancis/Corpus">000790</idno><idno type="wicri:Area/PascalFrancis/Curation">000200</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000757</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000757</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development</title><author><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y. M. Dennis" last="Lo">Y. M. Dennis Lo</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>5 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>7 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>5 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author><author><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>7 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>Hong Kong</country><placeName><settlement type="city">Sha Tin</settlement></placeName><orgName type="university">Université chinoise de Hong Kong</orgName></affiliation></author></analytic><series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochemistry</term><term>Clinical biology</term><term>Coronavirus</term><term>Development</term><term>Glycoprotein</term><term>Immunoprophylaxis</term><term>Molecular biology</term><term>Peptides</term><term>Prevention</term><term>Severe acute respiratory syndrome</term><term>Synthetic product</term><term>Vaccine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Produit synthétique</term><term>Peptide</term><term>Syndrome respiratoire aigu sévère</term><term>Coronavirus</term><term>Glycoprotéine</term><term>Vaccin</term><term>Immunoprophylaxie</term><term>Prévention</term><term>Développement</term><term>Biochimie</term><term>Biologie clinique</term><term>Biologie moléculaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term><term>Biochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0009-9147</s0></fA01><fA02 i1="01"><s0>CLCHAU</s0></fA02><fA03 i2="1"><s0>Clin. chem. : (Baltim. Md.)</s0></fA03><fA05><s2>50</s2></fA05><fA06><s2>6</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development</s1></fA08><fA11 i1="01" i2="1"><s1>CHOY (Wai-Yan)</s1></fA11><fA11 i1="02" i2="1"><s1>LIN (Shu-Guang)</s1></fA11><fA11 i1="03" i2="1"><s1>CHAN (Paul Kay-Sheung)</s1></fA11><fA11 i1="04" i2="1"><s1>TAM (John Siu-Lun)</s1></fA11><fA11 i1="05" i2="1"><s1>LO (Y. M. Dennis)</s1></fA11><fA11 i1="06" i2="1"><s1>CHU (Ida Miu-Ting)</s1></fA11><fA11 i1="07" i2="1"><s1>TSAI (Sau-Na)</s1></fA11><fA11 i1="08" i2="1"><s1>ZHONG (Ming-Qi)</s1></fA11><fA11 i1="09" i2="1"><s1>FUNG (Kwok-Pui)</s1></fA11><fA11 i1="10" i2="1"><s1>WAYE (Mary Miu-Yee)</s1></fA11><fA11 i1="11" i2="1"><s1>TSUI (Stephen Kwok-Wing)</s1></fA11><fA11 i1="12" i2="1"><s1>NG (Kai-On)</s1></fA11><fA11 i1="13" i2="1"><s1>SHAN (Zhi-Xin)</s1></fA11><fA11 i1="14" i2="1"><s1>MIN YANG</s1></fA11><fA11 i1="15" i2="1"><s1>WU (Yi-Long)</s1></fA11><fA11 i1="16" i2="1"><s1>LIN (Zhan-Yi)</s1></fA11><fA11 i1="17" i2="1"><s1>NGAI (Sai-Ming)</s1></fA11><fA14 i1="01"><s1>Molecular Biotechnology Program, Department of Biology and Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></fA14><fA14 i1="02"><s1>Research Center of Medical Sciences, Guangdong Provincial People's Hospital</s1><s2>Guangdong</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Microbiology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Chemical Pathology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>5 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Biology, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>7 aut.</sZ><sZ>17 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Biochemistry, The Chinese University of Hong Kong</s1><s2>Shatin</s2><s3>HKG</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></fA14><fA20><s1>1036-1042</s1></fA20><fA21><s1>2004</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>7603</s2><s5>354000113605350080</s5></fA43><fA44><s0>0000</s0><s1>© 2004 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>16 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>04-0547697</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Clinical chemistry : (Baltimore, Md.)</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.</s0></fC01><fC02 i1="01" i2="X"><s0>002B24</s0></fC02><fC02 i1="02" i2="X"><s0>002A02</s0></fC02><fC02 i1="03" i2="X"><s0>002A03</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Produit synthétique</s0><s5>02</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Synthetic product</s0><s5>02</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Producto sintético</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Peptide</s0><s5>03</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Peptides</s0><s5>03</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Péptido</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>05</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>05</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>05</s5></fC03><fC03 i1="04" i2="X" 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l="SPA"><s0>Prevención</s0><s5>12</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Développement</s0><s5>14</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Development</s0><s5>14</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Desarrollo</s0><s5>14</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Biochimie</s0><s5>15</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Biochemistry</s0><s5>15</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Bioquímica</s0><s5>15</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Biologie clinique</s0><s5>17</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Clinical biology</s0><s5>17</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Biología clínica</s0><s5>17</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Biologie moléculaire</s0><s5>18</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Molecular biology</s0><s5>18</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Biología molecular</s0><s5>18</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0><s2>NM</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Viral 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l="ENG"><s0>Respiratory disease</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>38</s5></fC07><fN21><s1>313</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><country name="Hong Kong"><noRegion><name sortKey="Choy, Wai Yan" sort="Choy, Wai Yan" uniqKey="Choy W" first="Wai-Yan" last="Choy">Wai-Yan Choy</name></noRegion><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name><name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name><name sortKey="Fung, Kwok Pui" sort="Fung, Kwok Pui" uniqKey="Fung K" first="Kwok-Pui" last="Fung">Kwok-Pui Fung</name><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y. M. Dennis" last="Lo">Y. M. Dennis Lo</name><name sortKey="Ng, Kai On" sort="Ng, Kai On" uniqKey="Ng K" first="Kai-On" last="Ng">Kai-On Ng</name><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name><name sortKey="Ngai, Sai Ming" sort="Ngai, Sai Ming" uniqKey="Ngai S" first="Sai-Ming" last="Ngai">Sai-Ming Ngai</name><name sortKey="Tam, John Siu Lun" sort="Tam, John Siu Lun" uniqKey="Tam J" first="John Siu-Lun" last="Tam">John Siu-Lun Tam</name><name sortKey="Tsai, Sau Na" sort="Tsai, Sau Na" uniqKey="Tsai S" first="Sau-Na" last="Tsai">Sau-Na Tsai</name><name sortKey="Tsui, Stephen Kwok Wing" sort="Tsui, Stephen Kwok Wing" uniqKey="Tsui S" first="Stephen Kwok-Wing" last="Tsui">Stephen Kwok-Wing Tsui</name><name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name><name sortKey="Zhong, Ming Qi" sort="Zhong, Ming Qi" uniqKey="Zhong M" first="Ming-Qi" last="Zhong">Ming-Qi Zhong</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Shu Guang" sort="Lin, Shu Guang" uniqKey="Lin S" first="Shu-Guang" last="Lin">Shu-Guang Lin</name></noRegion><name sortKey="Lin, Zhan Yi" sort="Lin, Zhan Yi" uniqKey="Lin Z" first="Zhan-Yi" last="Lin">Zhan-Yi Lin</name><name sortKey="Min Yang" sort="Min Yang" uniqKey="Min Yang" last="Min Yang">MIN YANG</name><name sortKey="Shan, Zhi Xin" sort="Shan, Zhi Xin" uniqKey="Shan Z" first="Zhi-Xin" last="Shan">Zhi-Xin Shan</name><name sortKey="Wu, Yi Long" sort="Wu, Yi Long" uniqKey="Wu Y" first="Yi-Long" last="Wu">Yi-Long Wu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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