SrasV1, Ncbi, Merge, bibRecord, 000719

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.

Identifieur interne : 000719 ( Ncbi/Merge ); précédent : 000718; suivant : 000720

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.

Auteurs : Wai-Yan Choy [République populaire de Chine] ; Shu-Guang Lin ; Paul Kay-Sheung Chan ; John Siu-Lun Tam ; Y M Dennis Lo ; Ida Miu-Ting Chu ; Sau-Na Tsai ; Ming-Qi Zhong ; Kwok-Pui Fung ; Mary Miu-Yee Waye ; Stephen Kwok-Wing Tsui ; Kai-On Ng ; Zhi-Xin Shan ; Min Yang ; Yi-Long Wu ; Zhan-Yi Lin ; Sai-Ming Ngai

Source :

Clinical chemistry [ 0009-9147 ] ; 2004.

RBID : pubmed:15044316

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Haplorhini, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Peptides (chemical synthesis), Peptides (chemistry), Peptides (immunology), Rabbits, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccination, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
MESH :
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Peptides, Viral Envelope Proteins, Viral Vaccines.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Haplorhini, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Rabbits, Spike Glycoprotein, Coronavirus, Vaccination.

Abstract

The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein.

DOI: 10.1373/clinchem.2003.029801
PubMed: 15044316

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002E79
to stream PubMed, to step Curation: 002E79
to stream PubMed, to step Checkpoint: 002A01

Links to Exploration step

pubmed:15044316

Le document en format XML

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<idno type="ISSN">0009-9147</idno>
<imprint><date when="2004" type="published">2004</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Haplorhini</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Peptides (chemical synthesis)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (immunology)</term>
<term>Rabbits</term>
<term>SARS Virus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides ()</term>
<term>Peptides (immunologie)</term>
<term>Peptides (synthèse chimique)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccination</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antiviraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Haplorhini</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Rabbits</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Haplorhini</term>
<term>Lapins</term>
<term>Microscopie confocale</term>
<term>Microscopie de fluorescence</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
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<front><div type="abstract" xml:lang="en">The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15044316</PMID>
<DateCompleted><Year>2004</Year>
<Month>07</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0009-9147</ISSN>
<JournalIssue CitedMedium="Print"><Volume>50</Volume>
<Issue>6</Issue>
<PubDate><Year>2004</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Clinical chemistry</Title>
<ISOAbbreviation>Clin. Chem.</ISOAbbreviation>
</Journal>
<ArticleTitle>Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.</ArticleTitle>
<Pagination><MedlinePgn>1036-42</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Choy</LastName>
<ForeName>Wai-Yan</ForeName>
<Initials>WY</Initials>
<AffiliationInfo><Affiliation>Molecular Biotechnology Program, Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Shu-Guang</ForeName>
<Initials>SG</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Paul Kay-Sheung</ForeName>
<Initials>PK</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tam</LastName>
<ForeName>John Siu-Lun</ForeName>
<Initials>JS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lo</LastName>
<ForeName>Y M Dennis</ForeName>
<Initials>YM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chu</LastName>
<ForeName>Ida Miu-Ting</ForeName>
<Initials>IM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tsai</LastName>
<ForeName>Sau-Na</ForeName>
<Initials>SN</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhong</LastName>
<ForeName>Ming-Qi</ForeName>
<Initials>MQ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Fung</LastName>
<ForeName>Kwok-Pui</ForeName>
<Initials>KP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Waye</LastName>
<ForeName>Mary Miu-Yee</ForeName>
<Initials>MM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tsui</LastName>
<ForeName>Stephen Kwok-Wing</ForeName>
<Initials>SK</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ng</LastName>
<ForeName>Kai-On</ForeName>
<Initials>KO</Initials>
</Author>
<Author ValidYN="Y"><LastName>Shan</LastName>
<ForeName>Zhi-Xin</ForeName>
<Initials>ZX</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Min</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Yi-Long</ForeName>
<Initials>YL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Zhan-Yi</ForeName>
<Initials>ZY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ngai</LastName>
<ForeName>Sai-Ming</ForeName>
<Initials>SM</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2004</Year>
<Month>03</Month>
<Day>25</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Clin Chem</MedlineTA>
<NlmUniqueID>9421549</NlmUniqueID>
<ISSNLinking>0009-9147</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010455">Peptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000882" MajorTopicYN="N">Haplorhini</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName>
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<MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName>
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<ArticleId IdType="pii">clinchem.2003.029801</ArticleId>
<ArticleId IdType="pmc">PMC7108158</ArticleId>
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<affiliations><list><country><li>République populaire de Chine</li>
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<settlement><li>Sha Tin</li>
</settlement>
<orgName><li>Université chinoise de Hong Kong</li>
</orgName>
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<tree><noCountry><name sortKey="Chan, Paul Kay Sheung" sort="Chan, Paul Kay Sheung" uniqKey="Chan P" first="Paul Kay-Sheung" last="Chan">Paul Kay-Sheung Chan</name>
<name sortKey="Chu, Ida Miu Ting" sort="Chu, Ida Miu Ting" uniqKey="Chu I" first="Ida Miu-Ting" last="Chu">Ida Miu-Ting Chu</name>
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   |texte=   Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.
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Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.

Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: perspective for SARS vaccine development.

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