High-efficiency detection of severe acute respiratory syndrome virus genetic material
Identifieur interne : 005B33 ( Main/Exploration ); précédent : 005B32; suivant : 005B34High-efficiency detection of severe acute respiratory syndrome virus genetic material
Auteurs : Manfred Weidmann [Allemagne] ; Paolo M. D. A. Zanotto [Allemagne] ; Friedemann Weber [Allemagne] ; Martin Spiegel [Allemagne] ; Hans Rheinhard Brodt [Brésil] ; Frank T. Hufert [Allemagne]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- virologie : Liquide de lavage bronchoalvéolaire.
- Pascal (Inist)
- Wicri :
- topic : Génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Bronchoalveolar Lavage Fluid.
- Humans, Sensitivity and Specificity.
Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
Url:
Affiliations:
- Allemagne, Brésil
- Bade-Wurtemberg, District de Darmstadt, District de Fribourg-en-Brisgau, Hesse (Land), État de São Paulo
- Francfort-sur-le-Main, Fribourg-en-Brisgau, São Paulo
- Université de São Paulo
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Le document en format XML
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<term>Microbiology</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
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<term>Sensitivity and Specificity</term>
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<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
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<front><div type="abstract" xml:lang="en">A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</div>
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<tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Weidmann, Manfred" sort="Weidmann, Manfred" uniqKey="Weidmann M" first="Manfred" last="Weidmann">Manfred Weidmann</name>
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<name sortKey="Zanotto, Paolo M D A" sort="Zanotto, Paolo M D A" uniqKey="Zanotto P" first="Paolo M. D. A." last="Zanotto">Paolo M. D. A. Zanotto</name>
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