High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material
Identifieur interne : 001646 ( Pmc/Checkpoint ); précédent : 001645; suivant : 001647High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material
Auteurs : Manfred Weidmann ; Paolo M. D. A. Zanotto ; Friedemann Weber ; Martin Spiegel ; Hans Rheinhard Brodt ; Frank T. HufertSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2004.
Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
Url:
DOI: 10.1128/JCM.42.6.2771-2773.2004
PubMed: 15184466
PubMed Central: 427811
Affiliations:
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PMC:427811Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">jcm</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
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<article-meta><article-id pub-id-type="pmid">15184466</article-id>
<article-id pub-id-type="pmc">427811</article-id>
<article-id pub-id-type="publisher-id">0085-04</article-id>
<article-id pub-id-type="doi">10.1128/JCM.42.6.2771-2773.2004</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Virology</subject>
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</article-categories>
<title-group><article-title>High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Weidmann</surname>
<given-names>Manfred</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zanotto</surname>
<given-names>Paolo M. D. A.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
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<contrib contrib-type="author"><name><surname>Weber</surname>
<given-names>Friedemann</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
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<contrib contrib-type="author"><name><surname>Spiegel</surname>
<given-names>Martin</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
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<contrib contrib-type="author"><name><surname>Brodt</surname>
<given-names>Hans Rheinhard</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hufert</surname>
<given-names>Frank T.</given-names>
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<xref ref-type="aff" rid="aff1">1</xref>
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<aff id="aff1">Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, 79008 Freiburg,<label>1</label>
Zentrum der Inneren Medizin, Medizinische Klinik III/Infektiologie, 60590 Frankfurt am Main, Germany,<label>3</label>
Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo, 05508-000 São Paulo, Brazil<label>2</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address: Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. Phone: 49.761.203.6610. Fax: 49.761.203.6608. E-mail: <email>weidmann@ukl.uni-freiburg.de</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>6</month>
<year>2004</year>
</pub-date>
<volume>42</volume>
<issue>6</issue>
<fpage>2771</fpage>
<lpage>2773</lpage>
<history><date date-type="received"><day>16</day>
<month>1</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>18</day>
<month>2</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>16</day>
<month>3</month>
<year>2004</year>
</date>
</history>
<copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement>
<copyright-year>2004</copyright-year>
<abstract><p>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Brodt, Hans Rheinhard" sort="Brodt, Hans Rheinhard" uniqKey="Brodt H" first="Hans Rheinhard" last="Brodt">Hans Rheinhard Brodt</name>
<name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name>
<name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name>
<name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name>
<name sortKey="Weidmann, Manfred" sort="Weidmann, Manfred" uniqKey="Weidmann M" first="Manfred" last="Weidmann">Manfred Weidmann</name>
<name sortKey="Zanotto, Paolo M D A" sort="Zanotto, Paolo M D A" uniqKey="Zanotto P" first="Paolo M. D. A." last="Zanotto">Paolo M. D. A. Zanotto</name>
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