High-efficiency detection of severe acute respiratory syndrome virus genetic material.
Identifieur interne : 002D47 ( PubMed/Curation ); précédent : 002D46; suivant : 002D48High-efficiency detection of severe acute respiratory syndrome virus genetic material.
Auteurs : Manfred Weidmann [Allemagne] ; Paolo M D A. Zanotto ; Friedemann Weber ; Martin Spiegel ; Hans Rheinhard Brodt ; Frank T. HufertSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- virologie : Liquide de lavage bronchoalvéolaire.
- Humains, RT-PCR, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Bronchoalveolar Lavage Fluid.
- Humans, Sensitivity and Specificity.
Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
DOI: 10.1128/JCM.42.6.2771-2773.2004
PubMed: 15184466
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pubmed:15184466Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. weidmann@ukl.uni-freiburg.de</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg</wicri:regionArea>
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<author><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name>
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<author><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name>
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<term>SARS Virus (genetics)</term>
<term>Sensitivity and Specificity</term>
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<term>Humains</term>
<term>Liquide de lavage bronchoalvéolaire (virologie)</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</div>
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<ArticleTitle>High-efficiency detection of severe acute respiratory syndrome virus genetic material.</ArticleTitle>
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<Abstract><AbstractText>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</AbstractText>
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