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High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material

Identifieur interne : 000650 ( Pmc/Curation ); précédent : 000649; suivant : 000651

High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material

Auteurs : Manfred Weidmann ; Paolo M. D. A. Zanotto ; Friedemann Weber ; Martin Spiegel ; Hans Rheinhard Brodt ; Frank T. Hufert

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2004.

RBID : PMC:427811

Abstract

A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log₁₀ times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log₁₀ times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC427811

DOI: 10.1128/JCM.42.6.2771-2773.2004
PubMed: 15184466
PubMed Central: 427811

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PMC:427811

Le document en format XML

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<author><name sortKey="Weidmann, Manfred" sort="Weidmann, Manfred" uniqKey="Weidmann M" first="Manfred" last="Weidmann">Manfred Weidmann</name>
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<author><name sortKey="Zanotto, Paolo M D A" sort="Zanotto, Paolo M D A" uniqKey="Zanotto P" first="Paolo M. D. A." last="Zanotto">Paolo M. D. A. Zanotto</name>
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<author><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name>
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<author><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name>
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<author><name sortKey="Brodt, Hans Rheinhard" sort="Brodt, Hans Rheinhard" uniqKey="Brodt H" first="Hans Rheinhard" last="Brodt">Hans Rheinhard Brodt</name>
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<author><name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name>
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<author><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name>
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<author><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name>
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<author><name sortKey="Brodt, Hans Rheinhard" sort="Brodt, Hans Rheinhard" uniqKey="Brodt H" first="Hans Rheinhard" last="Brodt">Hans Rheinhard Brodt</name>
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<author><name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name>
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<series><title level="j">Journal of Clinical Microbiology</title>
<idno type="ISSN">0095-1137</idno>
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<front><div type="abstract" xml:lang="en"><p>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">jcm</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
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<article-meta><article-id pub-id-type="pmid">15184466</article-id>
<article-id pub-id-type="pmc">427811</article-id>
<article-id pub-id-type="publisher-id">0085-04</article-id>
<article-id pub-id-type="doi">10.1128/JCM.42.6.2771-2773.2004</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Virology</subject>
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<title-group><article-title>High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Weidmann</surname>
<given-names>Manfred</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
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<contrib contrib-type="author"><name><surname>Zanotto</surname>
<given-names>Paolo M. D. A.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Weber</surname>
<given-names>Friedemann</given-names>
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<xref ref-type="aff" rid="aff1">1</xref>
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<contrib contrib-type="author"><name><surname>Spiegel</surname>
<given-names>Martin</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
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<contrib contrib-type="author"><name><surname>Brodt</surname>
<given-names>Hans Rheinhard</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hufert</surname>
<given-names>Frank T.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
</contrib-group>
<aff id="aff1">Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, 79008 Freiburg,<label>1</label>
 Zentrum der Inneren Medizin, Medizinische Klinik III/Infektiologie, 60590 Frankfurt am Main, Germany,<label>3</label>
 Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo, 05508-000 São Paulo, Brazil<label>2</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address: Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. Phone: 49.761.203.6610. Fax: 49.761.203.6608. E-mail: <email>weidmann@ukl.uni-freiburg.de</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>6</month>
<year>2004</year>
</pub-date>
<volume>42</volume>
<issue>6</issue>
<fpage>2771</fpage>
<lpage>2773</lpage>
<history><date date-type="received"><day>16</day>
<month>1</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>18</day>
<month>2</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>16</day>
<month>3</month>
<year>2004</year>
</date>
</history>
<copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement>
<copyright-year>2004</copyright-year>
<abstract><p>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</p>
</abstract>
</article-meta>
</front>
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High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material

High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material

Source :

Abstract

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