High-efficiency detection of severe acute respiratory syndrome virus genetic material
Identifieur interne : 005B33 ( Main/Curation ); précédent : 005B32; suivant : 005B34High-efficiency detection of severe acute respiratory syndrome virus genetic material
Auteurs : Manfred Weidmann [Allemagne] ; Paolo M. D. A. Zanotto [Allemagne] ; Friedemann Weber [Allemagne] ; Martin Spiegel [Allemagne] ; Hans Rheinhard Brodt [Brésil] ; Frank T. Hufert [Allemagne]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- virologie : Liquide de lavage bronchoalvéolaire.
- Pascal (Inist)
- Wicri :
- topic : Génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Bronchoalveolar Lavage Fluid.
- Humans, Sensitivity and Specificity.
Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
Url:
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Pascal:04-0346528Le document en format XML
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<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bronchoalveolar Lavage Fluid (virology)</term>
<term>Detection</term>
<term>Genetics</term>
<term>Humans</term>
<term>Microbiology</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>Sensitivity and Specificity</term>
<term>Severe acute respiratory syndrome</term>
<term>Virus</term>
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<term>Humains</term>
<term>Liquide de lavage bronchoalvéolaire (virologie)</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
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<front><div type="abstract" xml:lang="en">A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</div>
</front>
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</placeName>
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<s2>05508-000, São Paulo</s2>
<s3>BRA</s3>
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<author><name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name>
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<s2>79008 Freiburg</s2>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
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<country>Allemagne</country>
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<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
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<front><div type="abstract" xml:lang="en">A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
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<front><div type="abstract" xml:lang="en"><p>A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub>
times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub>
times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</p>
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