SrasV1, Main, Exploration, bibRecord, 004431

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Identifieur interne : 004431 ( Main/Exploration ); précédent : 004430; suivant : 004432

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Auteurs : Jong-Soo Lee [Corée du Sud] ; Haryoung Poo [Corée du Sud] ; Dong P. Han [États-Unis] ; Seung-Pyo Hong [Corée du Sud] ; Kwang Kim [Corée du Sud] ; Michael W. Cho [États-Unis] ; Eun Kim [Corée du Sud] ; Moon-Hee Sung [Corée du Sud] ; Chul-Joong Kim [Corée du Sud]

Source :

Journal of virology [ 0022-538X ] ; 2006.

RBID : Pascal:06-0212591

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (sang), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Immunisation, Immunité muqueuse, Lactobacillus casei (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée C57BL, Séquence d'acides aminés, Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins synthétiques (immunologie), Virus du SRAS (immunologie).
MESH :
- génétique : Lactobacillus casei.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins synthétiques, Virus du SRAS.
- sang : Anticorps antiviraux.
Pascal (Inist)
- Animaux, Coronavirus, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Immunisation, Immunité muqueuse, Lactobacillus casei, Souris, Muqueuse, Immunisation, Souris de lignée C57BL, Séquence d'acides aminés, Tests de neutralisation, Vaccin, Protéine, Anticorps neutralisant, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Coronavirus, Immunity, Mucosal, Immunization, Lactobacillus casei, Lactobacillus casei (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred C57BL, Microbiology, Molecular Sequence Data, Mouse, Mucosa, Neutralization Tests, Neutralizing antibody, Protein, SARS Virus (immunology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Vaccine, Vaccines, Synthetic (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (immunology), Virology.
MESH :
- chemical , blood : Antibodies, Viral.
- genetics : Lactobacillus casei.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- Amino Acid Sequence, Animals, Immunity, Mucosal, Immunization, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, Spike Glycoprotein, Coronavirus.

Abstract

Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div>
</front>
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<affiliations><list><country><li>Corée du Sud</li>
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<name sortKey="Hong, Seung Pyo" sort="Hong, Seung Pyo" uniqKey="Hong S" first="Seung-Pyo" last="Hong">Seung-Pyo Hong</name>
<name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul-Joong" last="Kim">Chul-Joong Kim</name>
<name sortKey="Kim, Eun" sort="Kim, Eun" uniqKey="Kim E" first="Eun" last="Kim">Eun Kim</name>
<name sortKey="Kim, Kwang" sort="Kim, Kwang" uniqKey="Kim K" first="Kwang" last="Kim">Kwang Kim</name>
<name sortKey="Poo, Haryoung" sort="Poo, Haryoung" uniqKey="Poo H" first="Haryoung" last="Poo">Haryoung Poo</name>
<name sortKey="Sung, Moon Hee" sort="Sung, Moon Hee" uniqKey="Sung M" first="Moon-Hee" last="Sung">Moon-Hee Sung</name>
<name sortKey="Sung, Moon Hee" sort="Sung, Moon Hee" uniqKey="Sung M" first="Moon-Hee" last="Sung">Moon-Hee Sung</name>
</country>
<country name="États-Unis"><region name="Ohio"><name sortKey="Han, Dong P" sort="Han, Dong P" uniqKey="Han D" first="Dong P." last="Han">Dong P. Han</name>
</region>
<name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W." last="Cho">Michael W. Cho</name>
</country>
</tree>
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   |wiki=    Sante
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   |texte=   Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice
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This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

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Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

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