SrasV1, PascalFrancis, Corpus, bibRecord, 000513

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Identifieur interne : 000513 ( PascalFrancis/Corpus ); précédent : 000512; suivant : 000514

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Auteurs : Jong-Soo Lee ; Haryoung Poo ; Dong P. Han ; Seung-Pyo Hong ; Kwang Kim ; Michael W. Cho ; Eun Kim ; Moon-Hee Sung ; Chul-Joong Kim

Source :

Journal of virology [ 0022-538X ] ; 2006.

RBID : Pascal:06-0212591

Descripteurs français

Pascal (Inist)
- Coronavirus, Lactobacillus casei, Souris, Muqueuse, Immunisation, Vaccin, Protéine, Anticorps neutralisant, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Immunization, Lactobacillus casei, Microbiology, Mouse, Mucosa, Neutralizing antibody, Protein, Severe acute respiratory syndrome, Vaccine, Virology.

Abstract

Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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C01	`01`		`ENG`	@0 Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
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Format Inist (serveur)

NO :	PASCAL 06-0212591 INIST
ET :	Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice
AU :	LEE (Jong-Soo); POO (Haryoung); HAN (Dong P.); HONG (Seung-Pyo); KIM (Kwang); CHO (Michael W.); KIM (Eun); SUNG (Moon-Hee); KIM (Chul-Joong)
AF :	Bioleaders Corporation/Daejeon/Corée, République de (1 aut., 4 aut., 5 aut., 8 aut.); Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)/Daejeon/Corée, République de (2 aut.); Department of Medicine, Case Western Reserve University School of Medicine/Cleveland, Ohio/Etats-Unis (3 aut., 6 aut.); National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University/Daejeon/Corée, République de (7 aut., 9 aut.); Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University/Seoul/Corée, République de (8 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2006; Vol. 80; No. 8; Pp. 4079-4087; Bibl. 36 ref.
LA :	Anglais
EA :	Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
CC :	002A05C10; 002A05C07
FD :	Coronavirus; Lactobacillus casei; Souris; Muqueuse; Immunisation; Vaccin; Protéine; Anticorps neutralisant; Microbiologie; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bactérie; Rodentia; Mammalia; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie; Bactérie lactique
ED :	Coronavirus; Lactobacillus casei; Mouse; Mucosa; Immunization; Vaccine; Protein; Neutralizing antibody; Microbiology; Virology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bacteria; Rodentia; Mammalia; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease; Lactic acid bacteria
SD :	Coronavirus; Lactobacillus casei; Ratón; Mucosa; Inmunización; Vacuna; Proteína; anticuerpo neutralizante; Microbiología; Virología; Síndrome respiratorio agudo severo
LO :	INIST-13592.354000142800860390
ID :	06-0212591

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Pascal:06-0212591

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice</title>
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<author><name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W." last="Cho">Michael W. Cho</name>
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<author><name sortKey="Sung, Moon Hee" sort="Sung, Moon Hee" uniqKey="Sung M" first="Moon-Hee" last="Sung">Moon-Hee Sung</name>
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<author><name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul-Joong" last="Kim">Chul-Joong Kim</name>
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<series><title level="j" type="main">Journal of virology</title>
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<term>Neutralizing antibody</term>
<term>Protein</term>
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<front><div type="abstract" xml:lang="en">Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div>
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<fC01 i1="01" l="ENG"><s0>Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</s0>
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<server><NO>PASCAL 06-0212591 INIST</NO>
<ET>Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice</ET>
<AU>LEE (Jong-Soo); POO (Haryoung); HAN (Dong P.); HONG (Seung-Pyo); KIM (Kwang); CHO (Michael W.); KIM (Eun); SUNG (Moon-Hee); KIM (Chul-Joong)</AU>
<AF>Bioleaders Corporation/Daejeon/Corée, République de (1 aut., 4 aut., 5 aut., 8 aut.); Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)/Daejeon/Corée, République de (2 aut.); Department of Medicine, Case Western Reserve University School of Medicine/Cleveland, Ohio/Etats-Unis (3 aut., 6 aut.); National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University/Daejeon/Corée, République de (7 aut., 9 aut.); Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University/Seoul/Corée, République de (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2006; Vol. 80; No. 8; Pp. 4079-4087; Bibl. 36 ref.</SO>
<LA>Anglais</LA>
<EA>Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</EA>
<CC>002A05C10; 002A05C07</CC>
<FD>Coronavirus; Lactobacillus casei; Souris; Muqueuse; Immunisation; Vaccin; Protéine; Anticorps neutralisant; Microbiologie; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bactérie; Rodentia; Mammalia; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie; Bactérie lactique</FG>
<ED>Coronavirus; Lactobacillus casei; Mouse; Mucosa; Immunization; Vaccine; Protein; Neutralizing antibody; Microbiology; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bacteria; Rodentia; Mammalia; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease; Lactic acid bacteria</EG>
<SD>Coronavirus; Lactobacillus casei; Ratón; Mucosa; Inmunización; Vacuna; Proteína; anticuerpo neutralizante; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
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Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

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