Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice
Identifieur interne : 000513 ( PascalFrancis/Corpus ); précédent : 000512; suivant : 000514Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice
Auteurs : Jong-Soo Lee ; Haryoung Poo ; Dong P. Han ; Seung-Pyo Hong ; Kwang Kim ; Michael W. Cho ; Eun Kim ; Moon-Hee Sung ; Chul-Joong KimSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
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Format Inist (serveur)
NO : | PASCAL 06-0212591 INIST |
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ET : | Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice |
AU : | LEE (Jong-Soo); POO (Haryoung); HAN (Dong P.); HONG (Seung-Pyo); KIM (Kwang); CHO (Michael W.); KIM (Eun); SUNG (Moon-Hee); KIM (Chul-Joong) |
AF : | Bioleaders Corporation/Daejeon/Corée, République de (1 aut., 4 aut., 5 aut., 8 aut.); Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)/Daejeon/Corée, République de (2 aut.); Department of Medicine, Case Western Reserve University School of Medicine/Cleveland, Ohio/Etats-Unis (3 aut., 6 aut.); National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University/Daejeon/Corée, République de (7 aut., 9 aut.); Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University/Seoul/Corée, République de (8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2006; Vol. 80; No. 8; Pp. 4079-4087; Bibl. 36 ref. |
LA : | Anglais |
EA : | Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus. |
CC : | 002A05C10; 002A05C07 |
FD : | Coronavirus; Lactobacillus casei; Souris; Muqueuse; Immunisation; Vaccin; Protéine; Anticorps neutralisant; Microbiologie; Virologie; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bactérie; Rodentia; Mammalia; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie; Bactérie lactique |
ED : | Coronavirus; Lactobacillus casei; Mouse; Mucosa; Immunization; Vaccine; Protein; Neutralizing antibody; Microbiology; Virology; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bacteria; Rodentia; Mammalia; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease; Lactic acid bacteria |
SD : | Coronavirus; Lactobacillus casei; Ratón; Mucosa; Inmunización; Vacuna; Proteína; anticuerpo neutralizante; Microbiología; Virología; Síndrome respiratorio agudo severo |
LO : | INIST-13592.354000142800860390 |
ID : | 06-0212591 |
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Pascal:06-0212591Le document en format XML
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<front><div type="abstract" xml:lang="en">Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div>
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<fC03 i1="05" i2="X" l="ENG"><s0>Immunization</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Inmunización</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Vaccin</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Vaccine</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Vacuna</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Protéine</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Protein</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Proteína</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Anticorps neutralisant</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Neutralizing antibody</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>anticuerpo neutralizante</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Virologie</s0>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Virology</s0>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Virología</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Lactobacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Lactobacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Lactobacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fC07 i1="13" i2="X" l="FRE"><s0>Bactérie lactique</s0>
<s5>18</s5>
</fC07>
<fC07 i1="13" i2="X" l="ENG"><s0>Lactic acid bacteria</s0>
<s5>18</s5>
</fC07>
<fC07 i1="13" i2="X" l="SPA"><s0>Bacteria láctica</s0>
<s5>18</s5>
</fC07>
<fN21><s1>135</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 06-0212591 INIST</NO>
<ET>Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice</ET>
<AU>LEE (Jong-Soo); POO (Haryoung); HAN (Dong P.); HONG (Seung-Pyo); KIM (Kwang); CHO (Michael W.); KIM (Eun); SUNG (Moon-Hee); KIM (Chul-Joong)</AU>
<AF>Bioleaders Corporation/Daejeon/Corée, République de (1 aut., 4 aut., 5 aut., 8 aut.); Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)/Daejeon/Corée, République de (2 aut.); Department of Medicine, Case Western Reserve University School of Medicine/Cleveland, Ohio/Etats-Unis (3 aut., 6 aut.); National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University/Daejeon/Corée, République de (7 aut., 9 aut.); Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University/Seoul/Corée, République de (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2006; Vol. 80; No. 8; Pp. 4079-4087; Bibl. 36 ref.</SO>
<LA>Anglais</LA>
<EA>Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</EA>
<CC>002A05C10; 002A05C07</CC>
<FD>Coronavirus; Lactobacillus casei; Souris; Muqueuse; Immunisation; Vaccin; Protéine; Anticorps neutralisant; Microbiologie; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bactérie; Rodentia; Mammalia; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie; Bactérie lactique</FG>
<ED>Coronavirus; Lactobacillus casei; Mouse; Mucosa; Immunization; Vaccine; Protein; Neutralizing antibody; Microbiology; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Lactobacillaceae; Bacteria; Rodentia; Mammalia; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease; Lactic acid bacteria</EG>
<SD>Coronavirus; Lactobacillus casei; Ratón; Mucosa; Inmunización; Vacuna; Proteína; anticuerpo neutralizante; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-13592.354000142800860390</LO>
<ID>06-0212591</ID>
</server>
</inist>
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