Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.
Identifieur interne : 002295 ( PubMed/Corpus ); précédent : 002294; suivant : 002296Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.
Auteurs : Jong-Soo Lee ; Haryoung Poo ; Dong P. Han ; Seung-Pyo Hong ; Kwang Kim ; Michael W. Cho ; Eun Kim ; Moon-Hee Sung ; Chul-Joong KimSource :
- Journal of virology [ 0022-538X ] ; 2006.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Immunity, Mucosal, Immunization, Lactobacillus casei (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccines, Synthetic (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- genetics : Lactobacillus casei.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- Amino Acid Sequence, Animals, Immunity, Mucosal, Immunization, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, Spike Glycoprotein, Coronavirus.
Abstract
Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
DOI: 10.1128/JVI.80.8.4079-4087.2006
PubMed: 16571824
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pubmed:16571824Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.</title><author><name sortKey="Lee, Jong Soo" sort="Lee, Jong Soo" uniqKey="Lee J" first="Jong-Soo" last="Lee">Jong-Soo Lee</name><affiliation><nlm:affiliation>Bioleaders Corporation, Daejeon, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Poo, Haryoung" sort="Poo, Haryoung" uniqKey="Poo H" first="Haryoung" last="Poo">Haryoung Poo</name></author><author><name sortKey="Han, Dong P" sort="Han, Dong P" uniqKey="Han D" first="Dong P" last="Han">Dong P. Han</name></author><author><name sortKey="Hong, Seung Pyo" sort="Hong, Seung Pyo" uniqKey="Hong S" first="Seung-Pyo" last="Hong">Seung-Pyo Hong</name></author><author><name sortKey="Kim, Kwang" sort="Kim, Kwang" uniqKey="Kim K" first="Kwang" last="Kim">Kwang Kim</name></author><author><name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W" last="Cho">Michael W. Cho</name></author><author><name sortKey="Kim, Eun" sort="Kim, Eun" uniqKey="Kim E" first="Eun" last="Kim">Eun Kim</name></author><author><name sortKey="Sung, Moon Hee" sort="Sung, Moon Hee" uniqKey="Sung M" first="Moon-Hee" last="Sung">Moon-Hee Sung</name></author><author><name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul-Joong" last="Kim">Chul-Joong Kim</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16571824</idno><idno type="pmid">16571824</idno><idno type="doi">10.1128/JVI.80.8.4079-4087.2006</idno><idno type="wicri:Area/PubMed/Corpus">002295</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002295</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.</title><author><name sortKey="Lee, Jong Soo" sort="Lee, Jong Soo" uniqKey="Lee J" first="Jong-Soo" last="Lee">Jong-Soo Lee</name><affiliation><nlm:affiliation>Bioleaders Corporation, Daejeon, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Poo, Haryoung" sort="Poo, Haryoung" uniqKey="Poo H" first="Haryoung" last="Poo">Haryoung Poo</name></author><author><name sortKey="Han, Dong P" sort="Han, Dong P" uniqKey="Han D" first="Dong P" last="Han">Dong P. Han</name></author><author><name sortKey="Hong, Seung Pyo" sort="Hong, Seung Pyo" uniqKey="Hong S" first="Seung-Pyo" last="Hong">Seung-Pyo Hong</name></author><author><name sortKey="Kim, Kwang" sort="Kim, Kwang" uniqKey="Kim K" first="Kwang" last="Kim">Kwang Kim</name></author><author><name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W" last="Cho">Michael W. Cho</name></author><author><name sortKey="Kim, Eun" sort="Kim, Eun" uniqKey="Kim E" first="Eun" last="Kim">Eun Kim</name></author><author><name sortKey="Sung, Moon Hee" sort="Sung, Moon Hee" uniqKey="Sung M" first="Moon-Hee" last="Sung">Moon-Hee Sung</name></author><author><name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul-Joong" last="Kim">Chul-Joong Kim</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Immunity, Mucosal</term><term>Immunization</term><term>Lactobacillus casei (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, Synthetic (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lactobacillus casei</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>SARS Virus</term><term>Vaccines, Synthetic</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Immunity, Mucosal</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16571824</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>80</Volume><Issue>8</Issue><PubDate><Year>2006</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.</ArticleTitle><Pagination><MedlinePgn>4079-87</MedlinePgn></Pagination><Abstract><AbstractText>Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jong-Soo</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Bioleaders Corporation, Daejeon, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poo</LastName><ForeName>Haryoung</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Dong P</ForeName><Initials>DP</Initials></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Seung-Pyo</ForeName><Initials>SP</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Kwang</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Michael W</ForeName><Initials>MW</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Eun</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>Moon-Hee</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Chul-Joong</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R21 AI059217</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 AI057160</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014614">Vaccines, Synthetic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope 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