SrasV1, PascalFrancis, Curation, bibRecord, 000477

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Identifieur interne : 000477 ( PascalFrancis/Curation ); précédent : 000476; suivant : 000478

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Auteurs : Jong-Soo Lee [Corée du Sud] ; Haryoung Poo [Corée du Sud] ; Dong P. Han [États-Unis] ; Seung-Pyo Hong [Corée du Sud] ; Kwang Kim [Corée du Sud] ; Michael W. Cho [États-Unis] ; Eun Kim [Corée du Sud] ; Moon-Hee Sung [Corée du Sud] ; Chul-Joong Kim [Corée du Sud]

Source :

Journal of virology [ 0022-538X ] ; 2006.

RBID : Pascal:06-0212591

Descripteurs français

Pascal (Inist)
- Coronavirus, Lactobacillus casei, Souris, Muqueuse, Immunisation, Vaccin, Protéine, Anticorps neutralisant, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Coronavirus, Immunization, Lactobacillus casei, Microbiology, Mouse, Mucosa, Neutralizing antibody, Protein, Severe acute respiratory syndrome, Vaccine, Virology.

Abstract

Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.

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A11	`02`	`1`		`@1 POO (Haryoung)`
A11	`03`	`1`		`@1 HAN (Dong P.)`
A11	`04`	`1`		`@1 HONG (Seung-Pyo)`
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A11	`06`	`1`		`@1 CHO (Michael W.)`
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A14	`04`			`@1 National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University @2 Daejeon @3 KOR @Z 7 aut. @Z 9 aut.`
A14	`05`			`@1 Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University @2 Seoul @3 KOR @Z 8 aut.`
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C01	`01`		`ENG`	@0 Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
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C03	`02`	`X`	`FRE`	`@0 Lactobacillus casei @2 NS @5 02`
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C03	`05`	`X`	`FRE`	`@0 Immunisation @5 06`
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C03	`05`	`X`	`SPA`	`@0 Inmunización @5 06`
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C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
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C07	`04`	`X`	`FRE`	`@0 Lactobacillaceae @2 NS`
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C07	`05`	`X`	`FRE`	`@0 Bactérie`
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C07	`06`	`X`	`SPA`	`@0 Rodentia @2 NS`
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C07	`07`	`X`	`ENG`	`@0 Mammalia @2 NS`
C07	`07`	`X`	`SPA`	`@0 Mammalia @2 NS`
C07	`08`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`08`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`08`	`X`	`SPA`	`@0 Vertebrata @2 NS`
C07	`09`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 13`
C07	`09`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`09`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`10`	`X`	`FRE`	`@0 Virose`
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C07	`13`	`X`	`FRE`	`@0 Bactérie lactique @5 18`
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C07	`13`	`X`	`SPA`	`@0 Bacteria láctica @5 18`
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Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000513

Links to Exploration step

Pascal:06-0212591

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Immunization</term>
<term>Lactobacillus casei</term>
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<term>Mouse</term>
<term>Mucosa</term>
<term>Neutralizing antibody</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Vaccine</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Lactobacillus casei</term>
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<term>Muqueuse</term>
<term>Immunisation</term>
<term>Vaccin</term>
<term>Protéine</term>
<term>Anticorps neutralisant</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
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<front><div type="abstract" xml:lang="en">Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0>
</fA01>
<fA03 i2="1"><s0>J. virol.</s0>
</fA03>
<fA05><s2>80</s2>
</fA05>
<fA06><s2>8</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LEE (Jong-Soo)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>POO (Haryoung)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>HAN (Dong P.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>HONG (Seung-Pyo)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>KIM (Kwang)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>CHO (Michael W.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>KIM (Eun)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>SUNG (Moon-Hee)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>KIM (Chul-Joong)</s1>
</fA11>
<fA14 i1="01"><s1>Bioleaders Corporation</s1>
<s2>Daejeon</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)</s1>
<s2>Daejeon</s2>
<s3>KOR</s3>
<sZ>2 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Medicine, Case Western Reserve University School of Medicine</s1>
<s2>Cleveland, Ohio</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>National Research Laboratory (NRL), College of Veterinary Medicine, Chungnam National University</s1>
<s2>Daejeon</s2>
<s3>KOR</s3>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Bio and Nanochemistry, College of Natural Sciences, Kookmin University</s1>
<s2>Seoul</s2>
<s3>KOR</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>4079-4087</s1>
</fA20>
<fA21><s1>2006</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13592</s2>
<s5>354000142800860390</s5>
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<fA44><s0>0000</s0>
<s1>© 2006 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>36 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>06-0212591</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</s0>
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<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A05C07</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Lactobacillus casei</s0>
<s2>NS</s2>
<s5>02</s5>
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<fC03 i1="02" i2="X" l="ENG"><s0>Lactobacillus casei</s0>
<s2>NS</s2>
<s5>02</s5>
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<fC03 i1="02" i2="X" l="SPA"><s0>Lactobacillus casei</s0>
<s2>NS</s2>
<s5>02</s5>
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<fC03 i1="03" i2="X" l="FRE"><s0>Souris</s0>
<s5>03</s5>
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<fC03 i1="03" i2="X" l="ENG"><s0>Mouse</s0>
<s5>03</s5>
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<fC03 i1="03" i2="X" l="SPA"><s0>Ratón</s0>
<s5>03</s5>
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<fC03 i1="04" i2="X" l="FRE"><s0>Muqueuse</s0>
<s5>05</s5>
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<fC03 i1="04" i2="X" l="ENG"><s0>Mucosa</s0>
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<fC03 i1="05" i2="X" l="FRE"><s0>Immunisation</s0>
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<s5>06</s5>
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<fC03 i1="05" i2="X" l="SPA"><s0>Inmunización</s0>
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<fC03 i1="06" i2="X" l="FRE"><s0>Vaccin</s0>
<s5>07</s5>
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<fC03 i1="06" i2="X" l="ENG"><s0>Vaccine</s0>
<s5>07</s5>
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<fC03 i1="06" i2="X" l="SPA"><s0>Vacuna</s0>
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<s5>08</s5>
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<fC03 i1="07" i2="X" l="ENG"><s0>Protein</s0>
<s5>08</s5>
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<s5>09</s5>
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<fC03 i1="09" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>10</s5>
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<fC03 i1="09" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>10</s5>
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<s5>11</s5>
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<s5>11</s5>
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<s5>11</s5>
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<fC03 i1="11" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
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<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
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<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Lactobacillaceae</s0>
<s2>NS</s2>
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<fC07 i1="04" i2="X" l="SPA"><s0>Lactobacillaceae</s0>
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<fC07 i1="05" i2="X" l="SPA"><s0>Bacteria</s0>
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<fC07 i1="06" i2="X" l="FRE"><s0>Rodentia</s0>
<s2>NS</s2>
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<fC07 i1="06" i2="X" l="ENG"><s0>Rodentia</s0>
<s2>NS</s2>
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<fC07 i1="06" i2="X" l="SPA"><s0>Rodentia</s0>
<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<fC07 i1="08" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
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<fC07 i1="08" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
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<fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
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<s5>13</s5>
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<fC07 i1="10" i2="X" l="FRE"><s0>Virose</s0>
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<fC07 i1="10" i2="X" l="ENG"><s0>Viral disease</s0>
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<fC07 i1="10" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
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<fC07 i1="12" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
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<fC07 i1="12" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
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<s5>18</s5>
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<s5>18</s5>
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<fC07 i1="13" i2="X" l="SPA"><s0>Bacteria láctica</s0>
<s5>18</s5>
</fC07>
<fN21><s1>135</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
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Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

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