Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.
Identifieur interne : 001435 ( Ncbi/Curation ); précédent : 001434; suivant : 001436Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.
Auteurs : Jong-Soo Lee [Corée du Sud] ; Haryoung Poo ; Dong P. Han ; Seung-Pyo Hong ; Kwang Kim ; Michael W. Cho ; Eun Kim ; Moon-Hee Sung ; Chul-Joong KimSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Immunisation, Immunité muqueuse, Lactobacillus casei (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée C57BL, Séquence d'acides aminés, Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins synthétiques (immunologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Lactobacillus casei.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins synthétiques, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Immunisation, Immunité muqueuse, Souris, Souris de lignée C57BL, Séquence d'acides aminés, Tests de neutralisation.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Immunity, Mucosal, Immunization, Lactobacillus casei (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccines, Synthetic (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- genetics : Lactobacillus casei.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- Amino Acid Sequence, Animals, Immunity, Mucosal, Immunization, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, Spike Glycoprotein, Coronavirus.
Abstract
Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.
DOI: 10.1128/JVI.80.8.4079-4087.2006
PubMed: 16571824
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Han</name></author><author><name sortKey="Hong, Seung Pyo" sort="Hong, Seung Pyo" uniqKey="Hong S" first="Seung-Pyo" last="Hong">Seung-Pyo Hong</name></author><author><name sortKey="Kim, Kwang" sort="Kim, Kwang" uniqKey="Kim K" first="Kwang" last="Kim">Kwang Kim</name></author><author><name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W" last="Cho">Michael W. 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Han</name></author><author><name sortKey="Hong, Seung Pyo" sort="Hong, Seung Pyo" uniqKey="Hong S" first="Seung-Pyo" last="Hong">Seung-Pyo Hong</name></author><author><name sortKey="Kim, Kwang" sort="Kim, Kwang" uniqKey="Kim K" first="Kwang" last="Kim">Kwang Kim</name></author><author><name sortKey="Cho, Michael W" sort="Cho, Michael W" uniqKey="Cho M" first="Michael W" last="Cho">Michael W. 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For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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