SrasV1, Main, Curation, bibRecord, 002F38

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Identifieur interne : 002F38 ( Main/Curation ); précédent : 002F37; suivant : 002F39

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Auteurs : Jason C. Huang [Taïwan] ; Ying-Feng Chang [Taïwan] ; Kuan-Hsuan Chen [Taïwan] ; Li-Chen Su [Taïwan] ; Chun-Wei Lee [Taïwan] ; Chii-Chang Chen [Taïwan] ; Yi-Ming Arthur Chen [Taïwan] ; Chien Chou [Taïwan]

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2009.

RBID : Pascal:09-0446030

Descripteurs français

KwdFr :
- Analyse chimique du sang (instrumentation), Analyse de panne d'appareillage, Conception d'appareillage, Humains, Protéines nucléocapside (sang), Résonance plasmonique de surface (instrumentation), Spectrométrie de fluorescence (instrumentation), Techniques de biocapteur (instrumentation), Technologie des fibres optiques (instrumentation), Virus du SRAS (métabolisme).
MESH :
- métabolisme : Virus du SRAS.
- sang : Analyse chimique du sang, Protéines nucléocapside, Résonance plasmonique de surface, Spectrométrie de fluorescence, Techniques de biocapteur, Technologie des fibres optiques.
Pascal (Inist)
- Analyse de panne d'appareillage, Conception d'appareillage, Détection, Humains, Syndrome respiratoire aigu sévère, Nucléocapside, Protéine, Homme, Coronavirus, Sérum, Plasmon surface, Fluorescence, Fibre optique, Biodétecteur, Or, Nanoparticule.
Wicri :
- topic : Homme, Or.

English descriptors

KwdEn :
- Biosensing Techniques (instrumentation), Biosensor, Blood Chemical Analysis (instrumentation), Coronavirus, Detection, Equipment Design, Equipment Failure Analysis, Fiber Optic Technology (instrumentation), Fluorescence, Gold, Human, Humans, Nanoparticle, Nucleocapsid, Nucleocapsid Proteins (blood), Optical fiber, Protein, SARS Virus (metabolism), Serum, Severe acute respiratory syndrome, Spectrometry, Fluorescence (instrumentation), Surface Plasmon Resonance (instrumentation), Surface plasmon.
MESH :
- chemical , blood : Nucleocapsid Proteins.
- instrumentation : Biosensing Techniques, Blood Chemical Analysis, Fiber Optic Technology, Spectrometry, Fluorescence, Surface Plasmon Resonance.
- metabolism : SARS Virus.
- Equipment Design, Equipment Failure Analysis, Humans.

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127111

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term>
<term>Biosensor</term>
<term>Blood Chemical Analysis (instrumentation)</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Fiber Optic Technology (instrumentation)</term>
<term>Fluorescence</term>
<term>Gold</term>
<term>Human</term>
<term>Humans</term>
<term>Nanoparticle</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid Proteins (blood)</term>
<term>Optical fiber</term>
<term>Protein</term>
<term>SARS Virus (metabolism)</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
<term>Spectrometry, Fluorescence (instrumentation)</term>
<term>Surface Plasmon Resonance (instrumentation)</term>
<term>Surface plasmon</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse chimique du sang (instrumentation)</term>
<term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Humains</term>
<term>Protéines nucléocapside (sang)</term>
<term>Résonance plasmonique de surface (instrumentation)</term>
<term>Spectrométrie de fluorescence (instrumentation)</term>
<term>Techniques de biocapteur (instrumentation)</term>
<term>Technologie des fibres optiques (instrumentation)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term>
<term>Blood Chemical Analysis</term>
<term>Fiber Optic Technology</term>
<term>Spectrometry, Fluorescence</term>
<term>Surface Plasmon Resonance</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
</keywords>
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</keywords>
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<term>Protéines nucléocapside</term>
<term>Résonance plasmonique de surface</term>
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<term>Techniques de biocapteur</term>
<term>Technologie des fibres optiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Humans</term>
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<term>Conception d'appareillage</term>
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<term>Syndrome respiratoire aigu sévère</term>
<term>Nucléocapside</term>
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<term>Fibre optique</term>
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<term>Or</term>
<term>Nanoparticule</term>
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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
</front>
</TEI>
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</affiliation>
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<author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name>
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</affiliation>
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<author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
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</affiliation>
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</inist:fA14>
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</affiliation>
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<sZ>8 aut.</sZ>
</inist:fA14>
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<series><title level="j" type="main">Biosensors & bioelectronics</title>
<title level="j" type="abbreviated">Biosens. bioelectron.</title>
<idno type="ISSN">0956-5663</idno>
<imprint><date when="2009">2009</date>
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</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensor</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>Fluorescence</term>
<term>Gold</term>
<term>Human</term>
<term>Nanoparticle</term>
<term>Nucleocapsid</term>
<term>Optical fiber</term>
<term>Protein</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
<term>Surface plasmon</term>
</keywords>
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<term>Syndrome respiratoire aigu sévère</term>
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<term>Homme</term>
<term>Coronavirus</term>
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<term>Plasmon surface</term>
<term>Fluorescence</term>
<term>Fibre optique</term>
<term>Biodétecteur</term>
<term>Or</term>
<term>Nanoparticule</term>
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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
</front>
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<author><name sortKey="Chen, Kuan Hsuan" sort="Chen, Kuan Hsuan" uniqKey="Chen K" first="Kuan-Hsuan" last="Chen">Kuan-Hsuan Chen</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</title>
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<country xml:lang="fr">Taïwan</country>
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</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff5">Department of Education and Research, Taipei City Hospital, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Education and Research, Taipei City Hospital, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Biophotonics, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
</author>
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<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name>
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<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea>
<wicri:noRegion>Taoyuan 320</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea>
<wicri:noRegion>Taoyuan 320</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Institute of Biophotonics, National Yang-Ming University, Taipei 112</wicri:regionArea>
<wicri:noRegion>Taipei 112</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea>
<wicri:noRegion>Taoyuan 320</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff7">Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333, Taiwan</nlm:aff>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333</wicri:regionArea>
<wicri:noRegion>Taoyuan 333</wicri:noRegion>
</affiliation>
</author>
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<series><title level="j">Biosensors & Bioelectronics</title>
<idno type="ISSN">0956-5663</idno>
<idno type="eISSN">1873-4235</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term>
<term>Blood Chemical Analysis (instrumentation)</term>
<term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Fiber Optic Technology (instrumentation)</term>
<term>Humans</term>
<term>Nucleocapsid Proteins (blood)</term>
<term>SARS Virus (metabolism)</term>
<term>Spectrometry, Fluorescence (instrumentation)</term>
<term>Surface Plasmon Resonance (instrumentation)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse chimique du sang (instrumentation)</term>
<term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Humains</term>
<term>Protéines nucléocapside (sang)</term>
<term>Résonance plasmonique de surface (instrumentation)</term>
<term>Spectrométrie de fluorescence (instrumentation)</term>
<term>Techniques de biocapteur (instrumentation)</term>
<term>Technologie des fibres optiques (instrumentation)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term>
<term>Blood Chemical Analysis</term>
<term>Fiber Optic Technology</term>
<term>Spectrometry, Fluorescence</term>
<term>Surface Plasmon Resonance</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Analyse chimique du sang</term>
<term>Protéines nucléocapside</term>
<term>Résonance plasmonique de surface</term>
<term>Spectrométrie de fluorescence</term>
<term>Techniques de biocapteur</term>
<term>Technologie des fibres optiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Humains</term>
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<front><div type="abstract" xml:lang="en"><p>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</p>
</div>
</front>
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   |texte=   Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
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Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

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