Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Identifieur interne : 000182 ( PascalFrancis/Corpus ); précédent : 000181; suivant : 000183Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Auteurs : Jason C. Huang ; Ying-Feng Chang ; Kuan-Hsuan Chen ; Li-Chen Su ; Chun-Wei Lee ; Chii-Chang Chen ; Yi-Ming Arthur Chen ; Chien ChouSource :
- Biosensors & bioelectronics [ 0956-5663 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
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Format Inist (serveur)
NO : | PASCAL 09-0446030 INIST |
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ET : | Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor |
AU : | HUANG (Jason C.); CHANG (Ying-Feng); CHEN (Kuan-Hsuan); SU (Li-Chen); LEE (Chun-Wei); CHEN (Chii-Chang); CHEN (Yi-Ming Arthur); CHOU (Chien) |
AF : | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 5 aut., 8 aut.); Institute of Biophotonics, National Yang-Ming University/Taipei 112/Taïwan (2 aut., 8 aut.); AIDS Prevention and Research Center, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 3 aut., 7 aut.); Department of Optics and Photonics, National Central University/Taoyuan 320/Taïwan (4 aut., 6 aut.); Department of Education and Research, Taipei City Hospital/Taipei 112/Taïwan (1 aut.); Institute of Microbiology and Immunology, National Yang-Ming University/Taipei 112/Taïwan (5 aut., 7 aut.); Graduate Institute of Electro-Optical Engineering, Chang Gung University/Taoyuan 333/Taïwan (8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Biosensors & bioelectronics; ISSN 0956-5663; Pays-Bas; Da. 2009; Vol. 25; No. 2; Pp. 320-325; Bibl. 1/2 p. |
LA : | Anglais |
EA : | In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection. |
CC : | 002A31C09B; 215 |
FD : | Détection; Syndrome respiratoire aigu sévère; Nucléocapside; Protéine; Homme; Coronavirus; Sérum; Plasmon surface; Fluorescence; Fibre optique; Biodétecteur; Or; Nanoparticule |
FG : | Virose; Infection; Coronaviridae; Nidovirales; Virus |
ED : | Detection; Severe acute respiratory syndrome; Nucleocapsid; Protein; Human; Coronavirus; Serum; Surface plasmon; Fluorescence; Optical fiber; Biosensor; Gold; Nanoparticle |
EG : | Viral disease; Infection; Coronaviridae; Nidovirales; Virus |
SD : | Detección; Síndrome respiratorio agudo severo; Nucleocápside; Proteína; Hombre; Coronavirus; Suero; Plasmón superficie; Fluorescencia; Fibra óptica; Biodetector; Oro; Nanopartícula |
LO : | INIST-20668.354000170302230090 |
ID : | 09-0446030 |
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Pascal:09-0446030Le document en format XML
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<term>Fluorescence</term>
<term>Gold</term>
<term>Human</term>
<term>Nanoparticle</term>
<term>Nucleocapsid</term>
<term>Optical fiber</term>
<term>Protein</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
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<term>Syndrome respiratoire aigu sévère</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Homme</term>
<term>Coronavirus</term>
<term>Sérum</term>
<term>Plasmon surface</term>
<term>Fluorescence</term>
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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
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<fC01 i1="01" l="ENG"><s0>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31C09B</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Détection</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Detection</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Protéine</s0>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Protein</s0>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Proteína</s0>
<s5>11</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Homme</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Human</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Hombre</s0>
<s5>12</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Sérum</s0>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Serum</s0>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Suero</s0>
<s5>19</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Plasmon surface</s0>
<s5>20</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Surface plasmon</s0>
<s5>20</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Plasmón superficie</s0>
<s5>20</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Fluorescence</s0>
<s5>21</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Fluorescence</s0>
<s5>21</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Fluorescencia</s0>
<s5>21</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Fibre optique</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Optical fiber</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Fibra óptica</s0>
<s5>22</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Biodétecteur</s0>
<s5>35</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Biosensor</s0>
<s5>35</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Biodetector</s0>
<s5>35</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Or</s0>
<s2>NC</s2>
<s5>36</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Gold</s0>
<s2>NC</s2>
<s5>36</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Oro</s0>
<s2>NC</s2>
<s5>36</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Nanoparticule</s0>
<s5>37</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Nanoparticle</s0>
<s5>37</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Nanopartícula</s0>
<s5>37</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fN21><s1>320</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 09-0446030 INIST</NO>
<ET>Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</ET>
<AU>HUANG (Jason C.); CHANG (Ying-Feng); CHEN (Kuan-Hsuan); SU (Li-Chen); LEE (Chun-Wei); CHEN (Chii-Chang); CHEN (Yi-Ming Arthur); CHOU (Chien)</AU>
<AF>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 5 aut., 8 aut.); Institute of Biophotonics, National Yang-Ming University/Taipei 112/Taïwan (2 aut., 8 aut.); AIDS Prevention and Research Center, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 3 aut., 7 aut.); Department of Optics and Photonics, National Central University/Taoyuan 320/Taïwan (4 aut., 6 aut.); Department of Education and Research, Taipei City Hospital/Taipei 112/Taïwan (1 aut.); Institute of Microbiology and Immunology, National Yang-Ming University/Taipei 112/Taïwan (5 aut., 7 aut.); Graduate Institute of Electro-Optical Engineering, Chang Gung University/Taoyuan 333/Taïwan (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Biosensors & bioelectronics; ISSN 0956-5663; Pays-Bas; Da. 2009; Vol. 25; No. 2; Pp. 320-325; Bibl. 1/2 p.</SO>
<LA>Anglais</LA>
<EA>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</EA>
<CC>002A31C09B; 215</CC>
<FD>Détection; Syndrome respiratoire aigu sévère; Nucléocapside; Protéine; Homme; Coronavirus; Sérum; Plasmon surface; Fluorescence; Fibre optique; Biodétecteur; Or; Nanoparticule</FD>
<FG>Virose; Infection; Coronaviridae; Nidovirales; Virus</FG>
<ED>Detection; Severe acute respiratory syndrome; Nucleocapsid; Protein; Human; Coronavirus; Serum; Surface plasmon; Fluorescence; Optical fiber; Biosensor; Gold; Nanoparticle</ED>
<EG>Viral disease; Infection; Coronaviridae; Nidovirales; Virus</EG>
<SD>Detección; Síndrome respiratorio agudo severo; Nucleocápside; Proteína; Hombre; Coronavirus; Suero; Plasmón superficie; Fluorescencia; Fibra óptica; Biodetector; Oro; Nanopartícula</SD>
<LO>INIST-20668.354000170302230090</LO>
<ID>09-0446030</ID>
</server>
</inist>
</record>
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