SrasV1, PascalFrancis, Corpus, bibRecord, 000182

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Identifieur interne : 000182 ( PascalFrancis/Corpus ); précédent : 000181; suivant : 000183

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Auteurs : Jason C. Huang ; Ying-Feng Chang ; Kuan-Hsuan Chen ; Li-Chen Su ; Chun-Wei Lee ; Chii-Chang Chen ; Yi-Ming Arthur Chen ; Chien Chou

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2009.

RBID : Pascal:09-0446030

Descripteurs français

Pascal (Inist)
- Détection, Syndrome respiratoire aigu sévère, Nucléocapside, Protéine, Homme, Coronavirus, Sérum, Plasmon surface, Fluorescence, Fibre optique, Biodétecteur, Or, Nanoparticule.

English descriptors

KwdEn :
- Biosensor, Coronavirus, Detection, Fluorescence, Gold, Human, Nanoparticle, Nucleocapsid, Optical fiber, Protein, Serum, Severe acute respiratory syndrome, Surface plasmon.

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 HUANG (Jason C.)`
A11	`02`	`1`		`@1 CHANG (Ying-Feng)`
A11	`03`	`1`		`@1 CHEN (Kuan-Hsuan)`
A11	`04`	`1`		`@1 SU (Li-Chen)`
A11	`05`	`1`		`@1 LEE (Chun-Wei)`
A11	`06`	`1`		`@1 CHEN (Chii-Chang)`
A11	`07`	`1`		`@1 CHEN (Yi-Ming Arthur)`
A11	`08`	`1`		`@1 CHOU (Chien)`
A14	`01`			`@1 Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 1 aut. @Z 5 aut. @Z 8 aut.`
A14	`02`			`@1 Institute of Biophotonics, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 2 aut. @Z 8 aut.`
A14	`03`			`@1 AIDS Prevention and Research Center, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 1 aut. @Z 3 aut. @Z 7 aut.`
A14	`04`			`@1 Department of Optics and Photonics, National Central University @2 Taoyuan 320 @3 TWN @Z 4 aut. @Z 6 aut.`
A14	`05`			`@1 Department of Education and Research, Taipei City Hospital @2 Taipei 112 @3 TWN @Z 1 aut.`
A14	`06`			`@1 Institute of Microbiology and Immunology, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 5 aut. @Z 7 aut.`
A14	`07`			`@1 Graduate Institute of Electro-Optical Engineering, Chang Gung University @2 Taoyuan 333 @3 TWN @Z 8 aut.`
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C01	`01`		`ENG`	@0 In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
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C03	`02`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 02`
C03	`02`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 02`
C03	`02`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 02`
C03	`03`	`X`	`FRE`	`@0 Nucléocapside @5 10`
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C03	`09`	`X`	`FRE`	`@0 Fluorescence @5 21`
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C03	`13`	`X`	`FRE`	`@0 Nanoparticule @5 37`
C03	`13`	`X`	`ENG`	`@0 Nanoparticle @5 37`
C03	`13`	`X`	`SPA`	`@0 Nanopartícula @5 37`
C07	`01`	`X`	`FRE`	`@0 Virose`
C07	`01`	`X`	`ENG`	`@0 Viral disease`
C07	`01`	`X`	`SPA`	`@0 Virosis`
C07	`02`	`X`	`FRE`	`@0 Infection`
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Format Inist (serveur)

NO :	PASCAL 09-0446030 INIST
ET :	Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
AU :	HUANG (Jason C.); CHANG (Ying-Feng); CHEN (Kuan-Hsuan); SU (Li-Chen); LEE (Chun-Wei); CHEN (Chii-Chang); CHEN (Yi-Ming Arthur); CHOU (Chien)
AF :	Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 5 aut., 8 aut.); Institute of Biophotonics, National Yang-Ming University/Taipei 112/Taïwan (2 aut., 8 aut.); AIDS Prevention and Research Center, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 3 aut., 7 aut.); Department of Optics and Photonics, National Central University/Taoyuan 320/Taïwan (4 aut., 6 aut.); Department of Education and Research, Taipei City Hospital/Taipei 112/Taïwan (1 aut.); Institute of Microbiology and Immunology, National Yang-Ming University/Taipei 112/Taïwan (5 aut., 7 aut.); Graduate Institute of Electro-Optical Engineering, Chang Gung University/Taoyuan 333/Taïwan (8 aut.)
DT :	Publication en série; Niveau analytique
SO :	Biosensors & bioelectronics; ISSN 0956-5663; Pays-Bas; Da. 2009; Vol. 25; No. 2; Pp. 320-325; Bibl. 1/2 p.
LA :	Anglais
EA :	In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
CC :	002A31C09B; 215
FD :	Détection; Syndrome respiratoire aigu sévère; Nucléocapside; Protéine; Homme; Coronavirus; Sérum; Plasmon surface; Fluorescence; Fibre optique; Biodétecteur; Or; Nanoparticule
FG :	Virose; Infection; Coronaviridae; Nidovirales; Virus
ED :	Detection; Severe acute respiratory syndrome; Nucleocapsid; Protein; Human; Coronavirus; Serum; Surface plasmon; Fluorescence; Optical fiber; Biosensor; Gold; Nanoparticle
EG :	Viral disease; Infection; Coronaviridae; Nidovirales; Virus
SD :	Detección; Síndrome respiratorio agudo severo; Nucleocápside; Proteína; Hombre; Coronavirus; Suero; Plasmón superficie; Fluorescencia; Fibra óptica; Biodetector; Oro; Nanopartícula
LO :	INIST-20668.354000170302230090
ID :	09-0446030

Links to Exploration step

Pascal:09-0446030

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</title>
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<author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
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<affiliation><inist:fA14 i1="02"><s1>Institute of Biophotonics, National Yang-Ming University</s1>
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<series><title level="j" type="main">Biosensors & bioelectronics</title>
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<term>Fluorescence</term>
<term>Gold</term>
<term>Human</term>
<term>Nanoparticle</term>
<term>Nucleocapsid</term>
<term>Optical fiber</term>
<term>Protein</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
<term>Surface plasmon</term>
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<term>Coronavirus</term>
<term>Sérum</term>
<term>Plasmon surface</term>
<term>Fluorescence</term>
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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
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<fC02 i1="02" i2="X"><s0>215</s0>
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<server><NO>PASCAL 09-0446030 INIST</NO>
<ET>Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</ET>
<AU>HUANG (Jason C.); CHANG (Ying-Feng); CHEN (Kuan-Hsuan); SU (Li-Chen); LEE (Chun-Wei); CHEN (Chii-Chang); CHEN (Yi-Ming Arthur); CHOU (Chien)</AU>
<AF>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 5 aut., 8 aut.); Institute of Biophotonics, National Yang-Ming University/Taipei 112/Taïwan (2 aut., 8 aut.); AIDS Prevention and Research Center, National Yang-Ming University/Taipei 112/Taïwan (1 aut., 3 aut., 7 aut.); Department of Optics and Photonics, National Central University/Taoyuan 320/Taïwan (4 aut., 6 aut.); Department of Education and Research, Taipei City Hospital/Taipei 112/Taïwan (1 aut.); Institute of Microbiology and Immunology, National Yang-Ming University/Taipei 112/Taïwan (5 aut., 7 aut.); Graduate Institute of Electro-Optical Engineering, Chang Gung University/Taoyuan 333/Taïwan (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Biosensors & bioelectronics; ISSN 0956-5663; Pays-Bas; Da. 2009; Vol. 25; No. 2; Pp. 320-325; Bibl. 1/2 p.</SO>
<LA>Anglais</LA>
<EA>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</EA>
<CC>002A31C09B; 215</CC>
<FD>Détection; Syndrome respiratoire aigu sévère; Nucléocapside; Protéine; Homme; Coronavirus; Sérum; Plasmon surface; Fluorescence; Fibre optique; Biodétecteur; Or; Nanoparticule</FD>
<FG>Virose; Infection; Coronaviridae; Nidovirales; Virus</FG>
<ED>Detection; Severe acute respiratory syndrome; Nucleocapsid; Protein; Human; Coronavirus; Serum; Surface plasmon; Fluorescence; Optical fiber; Biosensor; Gold; Nanoparticle</ED>
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Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

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