SrasV1, PascalFrancis, Curation, bibRecord, 000806

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Identifieur interne : 000806 ( PascalFrancis/Curation ); précédent : 000805; suivant : 000807

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Auteurs : Jason C. Huang [Taïwan] ; Ying-Feng Chang [Taïwan] ; Kuan-Hsuan Chen [Taïwan] ; Li-Chen Su [Taïwan] ; Chun-Wei Lee [Taïwan] ; Chii-Chang Chen [Taïwan] ; Yi-Ming Arthur Chen [Taïwan] ; Chien Chou [Taïwan]

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2009.

RBID : Pascal:09-0446030

Descripteurs français

Pascal (Inist)
- Détection, Syndrome respiratoire aigu sévère, Nucléocapside, Protéine, Homme, Coronavirus, Sérum, Plasmon surface, Fluorescence, Fibre optique, Biodétecteur, Or, Nanoparticule.
Wicri :
- topic : Homme, Or.

English descriptors

KwdEn :
- Biosensor, Coronavirus, Detection, Fluorescence, Gold, Human, Nanoparticle, Nucleocapsid, Optical fiber, Protein, Serum, Severe acute respiratory syndrome, Surface plasmon.

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

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A08	`01`	`1`	`ENG`	`@1 Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor`
A11	`01`	`1`		`@1 HUANG (Jason C.)`
A11	`02`	`1`		`@1 CHANG (Ying-Feng)`
A11	`03`	`1`		`@1 CHEN (Kuan-Hsuan)`
A11	`04`	`1`		`@1 SU (Li-Chen)`
A11	`05`	`1`		`@1 LEE (Chun-Wei)`
A11	`06`	`1`		`@1 CHEN (Chii-Chang)`
A11	`07`	`1`		`@1 CHEN (Yi-Ming Arthur)`
A11	`08`	`1`		`@1 CHOU (Chien)`
A14	`01`			`@1 Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 1 aut. @Z 5 aut. @Z 8 aut.`
A14	`02`			`@1 Institute of Biophotonics, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 2 aut. @Z 8 aut.`
A14	`03`			`@1 AIDS Prevention and Research Center, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 1 aut. @Z 3 aut. @Z 7 aut.`
A14	`04`			`@1 Department of Optics and Photonics, National Central University @2 Taoyuan 320 @3 TWN @Z 4 aut. @Z 6 aut.`
A14	`05`			`@1 Department of Education and Research, Taipei City Hospital @2 Taipei 112 @3 TWN @Z 1 aut.`
A14	`06`			`@1 Institute of Microbiology and Immunology, National Yang-Ming University @2 Taipei 112 @3 TWN @Z 5 aut. @Z 7 aut.`
A14	`07`			`@1 Graduate Institute of Electro-Optical Engineering, Chang Gung University @2 Taoyuan 333 @3 TWN @Z 8 aut.`
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A21				`@1 2009`
A23	`01`			`@0 ENG`
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C01	`01`		`ENG`	@0 In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10⁴-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
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C03	`03`	`X`	`FRE`	`@0 Nucléocapside @5 10`
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C03	`05`	`X`	`FRE`	`@0 Homme @5 12`
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C03	`07`	`X`	`FRE`	`@0 Sérum @5 19`
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C03	`08`	`X`	`FRE`	`@0 Plasmon surface @5 20`
C03	`08`	`X`	`ENG`	`@0 Surface plasmon @5 20`
C03	`08`	`X`	`SPA`	`@0 Plasmón superficie @5 20`
C03	`09`	`X`	`FRE`	`@0 Fluorescence @5 21`
C03	`09`	`X`	`ENG`	`@0 Fluorescence @5 21`
C03	`09`	`X`	`SPA`	`@0 Fluorescencia @5 21`
C03	`10`	`X`	`FRE`	`@0 Fibre optique @5 22`
C03	`10`	`X`	`ENG`	`@0 Optical fiber @5 22`
C03	`10`	`X`	`SPA`	`@0 Fibra óptica @5 22`
C03	`11`	`X`	`FRE`	`@0 Biodétecteur @5 35`
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C03	`13`	`X`	`FRE`	`@0 Nanoparticule @5 37`
C03	`13`	`X`	`ENG`	`@0 Nanoparticle @5 37`
C03	`13`	`X`	`SPA`	`@0 Nanopartícula @5 37`
C07	`01`	`X`	`FRE`	`@0 Virose`
C07	`01`	`X`	`ENG`	`@0 Viral disease`
C07	`01`	`X`	`SPA`	`@0 Virosis`
C07	`02`	`X`	`FRE`	`@0 Infection`
C07	`02`	`X`	`ENG`	`@0 Infection`
C07	`02`	`X`	`SPA`	`@0 Infección`
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C07	`04`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`05`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`05`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`05`	`X`	`SPA`	`@0 Virus @2 NW`
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N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Le document en format XML

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<author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</title>
<author><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name>
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<author><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name>
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<author><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name>
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<s2>Taoyuan 320</s2>
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<sZ>4 aut.</sZ>
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<author><name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name>
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<author><name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Optics and Photonics, National Central University</s1>
<s2>Taoyuan 320</s2>
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<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1>
<s2>Taipei 112</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Institute of Microbiology and Immunology, National Yang-Ming University</s1>
<s2>Taipei 112</s2>
<s3>TWN</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1>
<s2>Taipei 112</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Biophotonics, National Yang-Ming University</s1>
<s2>Taipei 112</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1>
<s2>Taoyuan 333</s2>
<s3>TWN</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Biosensors & bioelectronics</title>
<title level="j" type="abbreviated">Biosens. bioelectron.</title>
<idno type="ISSN">0956-5663</idno>
<imprint><date when="2009">2009</date>
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<seriesStmt><title level="j" type="main">Biosensors & bioelectronics</title>
<title level="j" type="abbreviated">Biosens. bioelectron.</title>
<idno type="ISSN">0956-5663</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensor</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>Fluorescence</term>
<term>Gold</term>
<term>Human</term>
<term>Nanoparticle</term>
<term>Nucleocapsid</term>
<term>Optical fiber</term>
<term>Protein</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
<term>Surface plasmon</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Détection</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Homme</term>
<term>Coronavirus</term>
<term>Sérum</term>
<term>Plasmon surface</term>
<term>Fluorescence</term>
<term>Fibre optique</term>
<term>Biodétecteur</term>
<term>Or</term>
<term>Nanoparticule</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
<term>Or</term>
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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
</front>
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<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0956-5663</s0>
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<fA03 i2="1"><s0>Biosens. bioelectron.</s0>
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<fA05><s2>25</s2>
</fA05>
<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>HUANG (Jason C.)</s1>
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<fA11 i1="02" i2="1"><s1>CHANG (Ying-Feng)</s1>
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<fA11 i1="03" i2="1"><s1>CHEN (Kuan-Hsuan)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SU (Li-Chen)</s1>
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<fA11 i1="05" i2="1"><s1>LEE (Chun-Wei)</s1>
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<fA11 i1="06" i2="1"><s1>CHEN (Chii-Chang)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>CHEN (Yi-Ming Arthur)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>CHOU (Chien)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1>
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<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
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<fA14 i1="02"><s1>Institute of Biophotonics, National Yang-Ming University</s1>
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<fA14 i1="03"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1>
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<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
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<fA14 i1="04"><s1>Department of Optics and Photonics, National Central University</s1>
<s2>Taoyuan 320</s2>
<s3>TWN</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
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<fA14 i1="05"><s1>Department of Education and Research, Taipei City Hospital</s1>
<s2>Taipei 112</s2>
<s3>TWN</s3>
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<fA14 i1="06"><s1>Institute of Microbiology and Immunology, National Yang-Ming University</s1>
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<s3>TWN</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
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<fA14 i1="07"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1>
<s2>Taoyuan 333</s2>
<s3>TWN</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>320-325</s1>
</fA20>
<fA21><s1>2009</s1>
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<fA23 i1="01"><s0>ENG</s0>
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<s1>© 2009 INIST-CNRS. All rights reserved.</s1>
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<fC01 i1="01" l="ENG"><s0>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>
-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</s0>
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<s5>01</s5>
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<s2>NC</s2>
<s5>36</s5>
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<s2>NC</s2>
<s5>36</s5>
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<s2>NW</s2>
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Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

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