SrasV1, PubMed, Checkpoint, bibRecord, 001904

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.

Identifieur interne : 001904 ( PubMed/Checkpoint ); précédent : 001903; suivant : 001905

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.

Auteurs : Jason C. Huang [Taïwan] ; Ying-Feng Chang ; Kuan-Hsuan Chen ; Li-Chen Su ; Chun-Wei Lee ; Chii-Chang Chen ; Yi-Ming Arthur Chen ; Chien Chou

Source :

Biosensors & bioelectronics [ 1873-4235 ] ; 2009.

RBID : pubmed:19660929

Descripteurs français

English descriptors

KwdEn :
- Biosensing Techniques (instrumentation), Blood Chemical Analysis (instrumentation), Equipment Design, Equipment Failure Analysis, Fiber Optic Technology (instrumentation), Humans, Nucleocapsid Proteins (blood), SARS Virus (metabolism), Spectrometry, Fluorescence (instrumentation), Surface Plasmon Resonance (instrumentation).
MESH :
- chemical , blood : Nucleocapsid Proteins.
- instrumentation : Biosensing Techniques, Blood Chemical Analysis, Fiber Optic Technology, Spectrometry, Fluorescence, Surface Plasmon Resonance.
- metabolism : SARS Virus.
- Equipment Design, Equipment Failure Analysis, Humans.

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10(4)-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (approximately 1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

DOI: 10.1016/j.bios.2009.07.012
PubMed: 19660929

Affiliations:

Taïwan

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pubmed:19660929

Le document en format XML

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<front><div type="abstract" xml:lang="en">In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10(4)-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (approximately 1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</div>
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<affiliations><list><country><li>Taïwan</li>
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<tree><noCountry><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name>
<name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name>
<name sortKey="Chen, Kuan Hsuan" sort="Chen, Kuan Hsuan" uniqKey="Chen K" first="Kuan-Hsuan" last="Chen">Kuan-Hsuan Chen</name>
<name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name>
<name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name>
<name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name>
<name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name>
</noCountry>
<country name="Taïwan"><noRegion><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C" last="Huang">Jason C. Huang</name>
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Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.

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