Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Identifieur interne : 000D33 ( Pmc/Checkpoint ); précédent : 000D32; suivant : 000D34Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Auteurs : Jason C. Huang [Taïwan] ; Ying-Feng Chang [Taïwan] ; Kuan-Hsuan Chen [Taïwan] ; Li-Chen Su [Taïwan] ; Chun-Wei Lee [Taïwan] ; Chii-Chang Chen [Taïwan] ; Yi-Ming Arthur Chen [Taïwan] ; Chien Chou [Taïwan]Source :
- Biosensors & Bioelectronics [ 0956-5663 ] ; 2009.
Abstract
In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
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DOI: 10.1016/j.bios.2009.07.012
PubMed: 19660929
PubMed Central: 7127111
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Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Department of Education and Research, Taipei City Hospital, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Education and 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Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7">Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333</wicri:regionArea><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19660929</idno><idno type="pmc">7127111</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127111</idno><idno type="RBID">PMC:7127111</idno><idno type="doi">10.1016/j.bios.2009.07.012</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000E59</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E59</idno><idno type="wicri:Area/Pmc/Curation">000E59</idno><idno 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Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Department of Education and Research, Taipei City Hospital, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Education and Research, Taipei City Hospital, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Kuan Hsuan" sort="Chen, Kuan Hsuan" uniqKey="Chen K" first="Kuan-Hsuan" last="Chen">Kuan-Hsuan Chen</name><affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><affiliation wicri:level="1"><nlm:aff id="aff3">AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biophotonics, National Yang-Ming University, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Optics and Photonics, National Central University, Taoyuan 320</wicri:regionArea><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7">Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333</wicri:regionArea><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biosensors & Bioelectronics</title><idno type="ISSN">0956-5663</idno><idno type="eISSN">1873-4235</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abad Valle, P" uniqKey="Abad Valle P">P. Abad-Valle</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernandez-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-Garcia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, P K S" uniqKey="Chan P">P.K.S. Chan</name></author><author><name sortKey="Liu, E Y M" uniqKey="Liu E">E.Y.M. Liu</name></author><author><name sortKey="Leung, D T M" uniqKey="Leung D">D.T.M. Leung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, Y H" uniqKey="Chang Y">Y.-H. Chang</name></author><author><name sortKey="Chang, T C" uniqKey="Chang T">T.-C. Chang</name></author><author><name sortKey="Kao, E F" uniqKey="Kao E">E.-F. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biosens Bioelectron</journal-id><journal-id journal-id-type="iso-abbrev">Biosens Bioelectron</journal-id><journal-title-group><journal-title>Biosensors & Bioelectronics</journal-title></journal-title-group><issn pub-type="ppub">0956-5663</issn><issn pub-type="epub">1873-4235</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19660929</article-id><article-id pub-id-type="pmc">7127111</article-id><article-id pub-id-type="publisher-id">S0956-5663(09)00383-2</article-id><article-id pub-id-type="doi">10.1016/j.bios.2009.07.012</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Jason C.</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff3" ref-type="aff">c</xref><xref rid="aff5" ref-type="aff">e</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Chang</surname><given-names>Ying-Feng</given-names></name><xref rid="aff2" ref-type="aff">b</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Kuan-Hsuan</given-names></name><xref rid="aff3" ref-type="aff">c</xref><xref rid="aff6" ref-type="aff">f</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Su</surname><given-names>Li-Chen</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Chun-Wei</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff6" ref-type="aff">f</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Chii-Chang</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Yi-Ming Arthur</given-names></name><email>arthur@ym.edu.tw</email><xref rid="aff3" ref-type="aff">c</xref><xref rid="aff6" ref-type="aff">f</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Chou</surname><given-names>Chien</given-names></name><email>cchou@mail.cgu.edu.tw</email><xref rid="aff2" ref-type="aff">b</xref><xref rid="aff4" ref-type="aff">d</xref><xref rid="aff7" ref-type="aff">g</xref><xref rid="cor2" ref-type="corresp">⁎⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan</aff><aff id="aff2"><label>b</label>Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan</aff><aff id="aff3"><label>c</label>AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan</aff><aff id="aff4"><label>d</label>Department of Optics and Photonics, National Central University, Taoyuan 320, Taiwan</aff><aff id="aff5"><label>e</label>Department of Education and Research, Taipei City Hospital, Taipei 112, Taiwan</aff><aff id="aff6"><label>f</label>Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan</aff><aff id="aff7"><label>g</label>Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333, Taiwan</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author at: AIDS Prevention and Research Center, National Yang-Ming University, Taipei 112, Taiwan. Tel.: +886 2 28267193; fax: +886 2 28270576. <email>arthur@ym.edu.tw</email></corresp><corresp id="cor2"><label>⁎⁎</label>Corresponding author at: Graduate Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan 333, Taiwan. Tel.: +886 3 2118800x3677; fax: +886 3 2118507. <email>cchou@mail.cgu.edu.tw</email></corresp><fn id="fn1"><label>1</label><p>Equal contribution in this research.</p></fn></author-notes><pub-date pub-type="pmc-release"><day>17</day><month>7</month><year>2009</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>15</day><month>10</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>17</day><month>7</month><year>2009</year></pub-date><volume>25</volume><issue>2</issue><fpage>320</fpage><lpage>325</lpage><history><date date-type="received"><day>1</day><month>6</month><year>2009</year></date><date date-type="rev-recd"><day>8</day><month>7</month><year>2009</year></date><date date-type="accepted"><day>10</day><month>7</month><year>2009</year></date></history><permissions><copyright-statement>Copyright © 2009 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2009</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10<sup>4</sup>-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.</p></abstract><kwd-group><title>Keywords</title><kwd>Fiber-optic biosensor</kwd><kwd>Gold nanoparticle</kwd><kwd>Localized surface plasmon</kwd><kwd>Nucleocapsid protein</kwd><kwd>Severe acute respiratory syndrome (SARS)</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name></noRegion><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name><name sortKey="Chen, Chii Chang" sort="Chen, Chii Chang" uniqKey="Chen C" first="Chii-Chang" last="Chen">Chii-Chang Chen</name><name sortKey="Chen, Kuan Hsuan" sort="Chen, Kuan Hsuan" uniqKey="Chen K" first="Kuan-Hsuan" last="Chen">Kuan-Hsuan Chen</name><name sortKey="Chen, Kuan Hsuan" sort="Chen, Kuan Hsuan" uniqKey="Chen K" first="Kuan-Hsuan" last="Chen">Kuan-Hsuan Chen</name><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name><name sortKey="Chou, Chien" sort="Chou, Chien" uniqKey="Chou C" first="Chien" last="Chou">Chien Chou</name><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name><name sortKey="Lee, Chun Wei" sort="Lee, Chun Wei" uniqKey="Lee C" first="Chun-Wei" last="Lee">Chun-Wei Lee</name><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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