Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch
Identifieur interne : 000218 ( Main/Merge ); précédent : 000217; suivant : 000219Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch
Auteurs : Terry Fei Fan Ng ; Li-Fang Chen ; Yanchen Zhou ; Beth Shapiro ; Mathias Stiller ; Peter D. Heintzman ; Arvind Varsani [Nouvelle-Zélande, Afrique du Sud] ; Nikola O. Kondov ; Walt Wong ; Xutao Deng ; Thomas D. Andrews [Canada] ; Brian J. Moorman [Canada] ; Thomas Meulendyk [Canada] ; Glen Mackay [Canada] ; Robert L. Gilbertson ; Eric DelwartSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- geographic : Arctic Regions.
- virology : Feces.
- Animals, Genome, Viral, Molecular Sequence Data, Reindeer.
Abstract
Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserved in ice. Using reverse genetics, we reconstituted the DNA virus, which replicated and systemically spread in a model plant species. Under constant freezing conditions, encapsidated viral nucleic acids may therefore be preserved for centuries. Our finding indicates that cryogenically preserved materials can be repositories of ancient viral nucleic acids, which in turn allow molecular genetics to regenerate viruses to study their biology.
Url:
DOI: 10.1073/pnas.1410429111
PubMed: 25349412
PubMed Central: 4250163
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PMC:4250163Le document en format XML
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Gilbertson</name><affiliation><nlm:aff id="aff3">Department of Plant Pathology,<institution>University of California, Davis</institution>,<addr-line>CA</addr-line> 95616;</nlm:aff></affiliation></author><author><name sortKey="Delwart, Eric" sort="Delwart, Eric" uniqKey="Delwart E" first="Eric" last="Delwart">Eric Delwart</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Laboratory Medicine,<institution>University of California, San Francisco</institution>,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25349412</idno><idno type="pmc">4250163</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250163</idno><idno type="RBID">PMC:4250163</idno><idno type="doi">10.1073/pnas.1410429111</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000311</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000311</idno><idno type="wicri:Area/Pmc/Curation">000311</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000311</idno><idno type="wicri:Area/Pmc/Checkpoint">000083</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000083</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000154</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000154</idno><idno type="wicri:Area/PubMed/Curation">000154</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000154</idno><idno type="wicri:Area/PubMed/Checkpoint">000154</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000154</idno><idno type="wicri:Area/Ncbi/Merge">000D92</idno><idno type="wicri:Area/Ncbi/Curation">000D92</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D92</idno><idno type="wicri:doubleKey">0027-8424:2014:Ng T:preservation:of:viral</idno><idno type="wicri:Area/Main/Merge">000218</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch</title><author><name sortKey="Ng, Terry Fei Fan" sort="Ng, Terry Fei Fan" uniqKey="Ng T" first="Terry Fei Fan" last="Ng">Terry Fei Fan Ng</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Laboratory Medicine,<institution>University of California, San Francisco</institution>,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author><author><name sortKey="Chen, Li Fang" sort="Chen, Li Fang" uniqKey="Chen L" first="Li-Fang" last="Chen">Li-Fang Chen</name><affiliation><nlm:aff id="aff3">Department of Plant Pathology,<institution>University of California, Davis</institution>,<addr-line>CA</addr-line> 95616;</nlm:aff></affiliation></author><author><name sortKey="Zhou, Yanchen" sort="Zhou, Yanchen" uniqKey="Zhou Y" first="Yanchen" last="Zhou">Yanchen Zhou</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Laboratory Medicine,<institution>University of California, San Francisco</institution>,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author><author><name sortKey="Shapiro, Beth" sort="Shapiro, Beth" uniqKey="Shapiro B" first="Beth" last="Shapiro">Beth Shapiro</name><affiliation><nlm:aff id="aff4">Department of Ecology & Evolutionary Biology,<institution>University of California, Santa Cruz</institution>,<addr-line>CA</addr-line> 95064;</nlm:aff></affiliation></author><author><name sortKey="Stiller, Mathias" sort="Stiller, Mathias" uniqKey="Stiller M" first="Mathias" last="Stiller">Mathias Stiller</name><affiliation><nlm:aff id="aff4">Department of Ecology & Evolutionary Biology,<institution>University of California, Santa Cruz</institution>,<addr-line>CA</addr-line> 95064;</nlm:aff></affiliation></author><author><name sortKey="Heintzman, Peter D" sort="Heintzman, Peter D" uniqKey="Heintzman P" first="Peter D." last="Heintzman">Peter D. Heintzman</name><affiliation><nlm:aff id="aff4">Department of Ecology & Evolutionary Biology,<institution>University of California, Santa Cruz</institution>,<addr-line>CA</addr-line> 95064;</nlm:aff></affiliation></author><author><name sortKey="Varsani, Arvind" sort="Varsani, Arvind" uniqKey="Varsani A" first="Arvind" last="Varsani">Arvind Varsani</name><affiliation wicri:level="1"><nlm:aff id="aff5">School of Biological Sciences and Biomolecular Interaction Centre,<institution>University of Canterbury</institution>, Christchurch,<country>New Zealand</country>;</nlm:aff><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff6">Department of Plant Pathology and Emerging Pathogens Institute,<institution>University of Florida</institution>, Gainesville,<addr-line>FL</addr-line> 32611;</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7">Electron Microscope Unit, Division of Medical Biochemistry, Department of Clinical Laboratory Sciences,<institution>University of Cape Town</institution>, Rondebosch, Cape Town, 7701,<country>South Africa</country>;</nlm:aff><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kondov, Nikola O" sort="Kondov, Nikola O" uniqKey="Kondov N" first="Nikola O." last="Kondov">Nikola O. Kondov</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author><author><name sortKey="Wong, Walt" sort="Wong, Walt" uniqKey="Wong W" first="Walt" last="Wong">Walt Wong</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author><author><name sortKey="Deng, Xutao" sort="Deng, Xutao" uniqKey="Deng X" first="Xutao" last="Deng">Xutao Deng</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Laboratory Medicine,<institution>University of California, San Francisco</institution>,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author><author><name sortKey="Andrews, Thomas D" sort="Andrews, Thomas D" uniqKey="Andrews T" first="Thomas D." last="Andrews">Thomas D. Andrews</name><affiliation wicri:level="1"><nlm:aff id="aff8">Prince of Wales Northern Heritage Centre,<institution>Government of the Northwest Territories</institution>, Yellowknife, NT,<country>Canada</country> X1A2L9;</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Moorman, Brian J" sort="Moorman, Brian J" uniqKey="Moorman B" first="Brian J." last="Moorman">Brian J. Moorman</name><affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff9">Department of Geography,<institution>University of Calgary</institution>, Calgary, AB,<country>Canada</country> T2N1N4</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Meulendyk, Thomas" sort="Meulendyk, Thomas" uniqKey="Meulendyk T" first="Thomas" last="Meulendyk">Thomas Meulendyk</name><affiliation wicri:level="1"><nlm:aff id="aff10">Department of Physical and Environmental Sciences,<institution>University of Toronto, Scarborough</institution>, Toronto, Ontario,<country>Canada</country> M1C1A4</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Mackay, Glen" sort="Mackay, Glen" uniqKey="Mackay G" first="Glen" last="Mackay">Glen Mackay</name><affiliation wicri:level="1"><nlm:aff id="aff8">Prince of Wales Northern Heritage Centre,<institution>Government of the Northwest Territories</institution>, Yellowknife, NT,<country>Canada</country> X1A2L9;</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gilbertson, Robert L" sort="Gilbertson, Robert L" uniqKey="Gilbertson R" first="Robert L." last="Gilbertson">Robert L. Gilbertson</name><affiliation><nlm:aff id="aff3">Department of Plant Pathology,<institution>University of California, Davis</institution>,<addr-line>CA</addr-line> 95616;</nlm:aff></affiliation></author><author><name sortKey="Delwart, Eric" sort="Delwart, Eric" uniqKey="Delwart E" first="Eric" last="Delwart">Eric Delwart</name><affiliation><nlm:aff id="aff1"><institution>Blood Systems Research Institute</institution>, San Francisco,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Laboratory Medicine,<institution>University of California, San Francisco</institution>,<addr-line>CA</addr-line> 94118;</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Arctic Regions</term><term>Feces (virology)</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Reindeer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Arctic Regions</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Reindeer</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserved in ice. Using reverse genetics, we reconstituted the DNA virus, which replicated and systemically spread in a model plant species. Under constant freezing conditions, encapsidated viral nucleic acids may therefore be preserved for centuries. Our finding indicates that cryogenically preserved materials can be repositories of ancient viral nucleic acids, which in turn allow molecular genetics to regenerate viruses to study their biology.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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