Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection
Identifieur interne : 000F68 ( Main/Exploration ); précédent : 000F67; suivant : 000F69Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection
Auteurs : Rouba Hoteit ; Dina Shammaa ; Rami MahfouzSource :
- Gene Reports [ 2452-0144 ] ; 2016.
Abstract
Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.
This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.
46 respiratory specimens were tested using the qualitative one-step qRT-PCR
Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.
The
Url:
DOI: 10.1016/j.genrep.2016.04.004
PubMed: NONE
PubMed Central: 7104146
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Use of the <italic>Human Coronavirus 2012</italic> (<italic>MERS</italic>) <italic>GeneSig</italic> kit for MERS-CoV detection</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7104146</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104146</idno><idno type="RBID">PMC:7104146</idno><idno type="doi">10.1016/j.genrep.2016.04.004</idno><idno type="pmid">NONE</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000C91</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C91</idno><idno type="wicri:Area/Pmc/Curation">000C91</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C91</idno><idno type="wicri:Area/Pmc/Checkpoint">000963</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000963</idno><idno type="wicri:Area/Ncbi/Merge">002D48</idno><idno type="wicri:Area/Ncbi/Curation">002D48</idno><idno type="wicri:Area/Ncbi/Checkpoint">002D48</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:32289095</idno><idno type="wicri:Area/PubMed/Corpus">000F83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000F83</idno><idno type="wicri:Area/PubMed/Curation">000F83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000F83</idno><idno type="wicri:Area/PubMed/Checkpoint">000E57</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000E57</idno><idno type="wicri:Area/Ncbi/Merge">002799</idno><idno type="wicri:Area/Ncbi/Curation">002799</idno><idno type="wicri:Area/Ncbi/Checkpoint">002799</idno><idno type="wicri:doubleKey">2452-0144:2016:Hoteit R:use:of:the</idno><idno type="wicri:Area/Main/Merge">000F71</idno><idno type="wicri:Area/Main/Curation">000F68</idno><idno type="wicri:Area/Main/Exploration">000F68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Use of the <italic>Human Coronavirus 2012</italic> (<italic>MERS</italic>) <italic>GeneSig</italic> kit for MERS-CoV detection</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name></author></analytic><series><title level="j">Gene Reports</title><idno type="eISSN">2452-0144</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Introduction</title><p>Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</p></sec><sec><title>Aim</title><p>This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.</p></sec><sec><title>Methods</title><p>46 respiratory specimens were tested using the qualitative one-step qRT-PCR <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit (PrimerDesign™).</p></sec><sec><title>Results</title><p>Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.</p></sec><sec><title>Conclusion</title><p>The <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Abdallat, M M" uniqKey="Al Abdallat M">M.M. 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Hui</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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