Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection.
Identifieur interne : 000F83 ( PubMed/Curation ); précédent : 000F82; suivant : 000F84Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection.
Auteurs : Rouba Hoteit [Liban] ; Dina Shammaa [Liban] ; Rami Mahfouz [Liban]Source :
- Gene reports [ 2452-0144 ] ; 2016.
Abstract
Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.
DOI: 10.1016/j.genrep.2016.04.004
PubMed: 32289095
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(<i>MERS</i>
) <i>GeneSig</i>
kit for MERS-CoV detection.</title>
<author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation>
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<wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea>
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<author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name>
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) <i>GeneSig</i>
kit for MERS-CoV detection.</title>
<author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation>
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<front><div type="abstract" xml:lang="en">Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</div>
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<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>18</Day>
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<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">2452-0144</ISSN>
<JournalIssue CitedMedium="Print"><Volume>4</Volume>
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<Month>Sep</Month>
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<Title>Gene reports</Title>
<ISOAbbreviation>Gene Rep</ISOAbbreviation>
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<ArticleTitle>Use of the <i>Human Coronavirus 2012</i>
(<i>MERS</i>
) <i>GeneSig</i>
kit for MERS-CoV detection.</ArticleTitle>
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<Abstract><AbstractText Label="Introduction" NlmCategory="UNASSIGNED">Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</AbstractText>
<AbstractText Label="Aim" NlmCategory="UNASSIGNED">This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.</AbstractText>
<AbstractText Label="Methods" NlmCategory="UNASSIGNED">46 respiratory specimens were tested using the qualitative one-step qRT-PCR <i>GeneSig Human Coronavirus 2012</i>
(<i>MERS</i>
) kit (PrimerDesign™).</AbstractText>
<AbstractText Label="Results" NlmCategory="UNASSIGNED">Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.</AbstractText>
<AbstractText Label="Conclusion" NlmCategory="UNASSIGNED">The <i>GeneSig Human Coronavirus 2012</i>
(<i>MERS</i>
) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions.</AbstractText>
<CopyrightInformation>© 2016 Elsevier Inc. All rights reserved.</CopyrightInformation>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ACTB, Actin Beta</Keyword>
<Keyword MajorTopicYN="N">DNA, Deoxyribonucleic Acid</Keyword>
<Keyword MajorTopicYN="N">GeneSig kit</Keyword>
<Keyword MajorTopicYN="N">Lebanon</Keyword>
<Keyword MajorTopicYN="N">MERS-CoV</Keyword>
<Keyword MajorTopicYN="N">MERS-CoV, Middle East Respiratory Syndrome</Keyword>
<Keyword MajorTopicYN="N">ORF, Open Reading Frame</Keyword>
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<ReferenceList><Reference><Citation>Euro Surveill. 2014 Jun 12;19(23):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24957745</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2014 Aug 28;371(9):828-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25162889</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Pathol. 2015 Jan;235(2):175-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25294366</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Pathol. 2015 Jan;235(2):185-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25270030</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2014 May 31;383(9932):1869-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24857701</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):752-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23891402</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2014 Jan;52(1):67-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24153118</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MMWR Morb Mortal Wkly Rep. 2013 Jun 14;62(23):480-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23760190</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):E3900-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25197083</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2014 Jun;20(6):1012-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24857749</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Int J Infect Dis. 2014 Dec;29:301-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25303830</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Int J Infect Dis. 2013 Sep;17(9):e668-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23916548</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Clin Infect Dis. 2014 Nov 1;59(9):1225-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24829216</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Dec;87(24):13892-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24067970</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
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