Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection
Identifieur interne : 000963 ( Pmc/Checkpoint ); précédent : 000962; suivant : 000964Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection
Auteurs : Rouba Hoteit ; Dina Shammaa ; Rami MahfouzSource :
- Gene Reports [ 2452-0144 ] ; 2016.
Abstract
Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.
This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.
46 respiratory specimens were tested using the qualitative one-step qRT-PCR
Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.
The
Url:
DOI: 10.1016/j.genrep.2016.04.004
PubMed: NONE
PubMed Central: 7104146
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Use of the <italic>Human Coronavirus 2012</italic> (<italic>MERS</italic>) <italic>GeneSig</italic> kit for MERS-CoV detection</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7104146</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104146</idno><idno type="RBID">PMC:7104146</idno><idno type="doi">10.1016/j.genrep.2016.04.004</idno><idno type="pmid">NONE</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000C91</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C91</idno><idno type="wicri:Area/Pmc/Curation">000C91</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C91</idno><idno type="wicri:Area/Pmc/Checkpoint">000963</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000963</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Use of the <italic>Human Coronavirus 2012</italic> (<italic>MERS</italic>) <italic>GeneSig</italic> kit for MERS-CoV detection</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name></author></analytic><series><title level="j">Gene Reports</title><idno type="eISSN">2452-0144</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Introduction</title><p>Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</p></sec><sec><title>Aim</title><p>This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.</p></sec><sec><title>Methods</title><p>46 respiratory specimens were tested using the qualitative one-step qRT-PCR <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit (PrimerDesign™).</p></sec><sec><title>Results</title><p>Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.</p></sec><sec><title>Conclusion</title><p>The <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Abdallat, M M" uniqKey="Al Abdallat M">M.M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Gene Rep</journal-id><journal-id journal-id-type="iso-abbrev">Gene Rep</journal-id><journal-title-group><journal-title>Gene Reports</journal-title></journal-title-group><issn pub-type="epub">2452-0144</issn><publisher><publisher-name>Elsevier Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7104146</article-id><article-id pub-id-type="publisher-id">S2452-0144(16)30021-8</article-id><article-id pub-id-type="doi">10.1016/j.genrep.2016.04.004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Use of the <italic>Human Coronavirus 2012</italic> (<italic>MERS</italic>) <italic>GeneSig</italic> kit for MERS-CoV detection</article-title></title-group><contrib-group><contrib contrib-type="author" id="au0005"><name><surname>Hoteit</surname><given-names>Rouba</given-names></name></contrib><contrib contrib-type="author" id="au0010"><name><surname>Shammaa</surname><given-names>Dina</given-names></name></contrib><contrib contrib-type="author" id="au0015"><name><surname>Mahfouz</surname><given-names>Rami</given-names></name><email>rm11@aub.edu.lb</email><xref rid="cr0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="af0005">Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon</aff><author-notes><corresp id="cr0005"><label>⁎</label>Corresponding author at: American University of Beirut Medical Center (AUBMC), Department of Pathology & Laboratory Medicine, P.O BOX 11-0236, Riad El Solh, 1107 2020, Beirut, Lebanon. Tel.: + 961 3 737017; fax: + 961 1 370845. <email>rm11@aub.edu.lb</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>16</day><month>4</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>16</day><month>4</month><year>2016</year></pub-date><volume>4</volume><fpage>67</fpage><lpage>69</lpage><history><date date-type="received"><day>1</day><month>4</month><year>2016</year></date><date date-type="rev-recd"><day>5</day><month>4</month><year>2016</year></date><date date-type="accepted"><day>5</day><month>4</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="ab0005"><sec><title>Introduction</title><p>Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</p></sec><sec><title>Aim</title><p>This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.</p></sec><sec><title>Methods</title><p>46 respiratory specimens were tested using the qualitative one-step qRT-PCR <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit (PrimerDesign™).</p></sec><sec><title>Results</title><p>Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.</p></sec><sec><title>Conclusion</title><p>The <italic>GeneSig Human Coronavirus 2012</italic> (<italic>MERS</italic>) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions.</p></sec></abstract><abstract abstract-type="author-highlights" id="ab0015"><title>Highlights</title><p><list list-type="simple" id="l9005"><list-item id="u9005"><label>•</label><p id="p9020">Mortality due to MERS-CoV infection is common especially among immunocompromised patients</p></list-item><list-item id="u9010"><label>•</label><p id="p9025">Several essays have been utilized to detect the virus in an attempt to diagnose it as early as possible</p></list-item><list-item id="u9015"><label>•</label><p id="p9030">The Human Coronavirus 2012 GeneSig kit is a new kit that can be used for MERS-CoV detection in respiratory samples</p></list-item></list></p></abstract><kwd-group id="ks0005"><title>Abbreviations</title><kwd>MERS-CoV, Middle East Respiratory Syndrome</kwd><kwd>RNA, Ribonucleic Acid</kwd><kwd>RT-PCR, Reverse-Transcriptase Polymerase Chain Reaction</kwd><kwd>DNA, Deoxyribonucleic Acid</kwd><kwd>ORF, Open Reading Frame</kwd><kwd>ACTB, Actin Beta</kwd></kwd-group><kwd-group id="ks0010"><title>Keywords</title><kwd>MERS-CoV</kwd><kwd>Lebanon</kwd><kwd>GeneSig kit</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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