Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection.
Identifieur interne : 000E57 ( PubMed/Checkpoint ); précédent : 000E56; suivant : 000E58Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection.
Auteurs : Rouba Hoteit [Liban] ; Dina Shammaa [Liban] ; Rami Mahfouz [Liban]Source :
- Gene reports [ 2452-0144 ] ; 2016.
Abstract
Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.
DOI: 10.1016/j.genrep.2016.04.004
PubMed: 32289095
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Use of the <i>Human Coronavirus 2012</i> (<i>MERS</i>) <i>GeneSig</i> kit for MERS-CoV detection.</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:32289095</idno><idno type="pmid">32289095</idno><idno type="doi">10.1016/j.genrep.2016.04.004</idno><idno type="wicri:Area/PubMed/Corpus">000F83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000F83</idno><idno type="wicri:Area/PubMed/Curation">000F83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000F83</idno><idno type="wicri:Area/PubMed/Checkpoint">000E57</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000E57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Use of the <i>Human Coronavirus 2012</i> (<i>MERS</i>) <i>GeneSig</i> kit for MERS-CoV detection.</title><author><name sortKey="Hoteit, Rouba" sort="Hoteit, Rouba" uniqKey="Hoteit R" first="Rouba" last="Hoteit">Rouba Hoteit</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author><author><name sortKey="Shammaa, Dina" sort="Shammaa, Dina" uniqKey="Shammaa D" first="Dina" last="Shammaa">Dina Shammaa</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author><author><name sortKey="Mahfouz, Rami" sort="Mahfouz, Rami" uniqKey="Mahfouz R" first="Rami" last="Mahfouz">Rami Mahfouz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</nlm:affiliation><country xml:lang="fr">Liban</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020</wicri:regionArea><wicri:noRegion>1107-2020</wicri:noRegion></affiliation></author></analytic><series><title level="j">Gene reports</title><idno type="ISSN">2452-0144</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32289095</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">2452-0144</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><PubDate><Year>2016</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Gene reports</Title><ISOAbbreviation>Gene Rep</ISOAbbreviation></Journal><ArticleTitle>Use of the <i>Human Coronavirus 2012</i> (<i>MERS</i>) <i>GeneSig</i> kit for MERS-CoV detection.</ArticleTitle><Pagination><MedlinePgn>67-69</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.genrep.2016.04.004</ELocationID><Abstract><AbstractText Label="Introduction" NlmCategory="UNASSIGNED">Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation.</AbstractText><AbstractText Label="Aim" NlmCategory="UNASSIGNED">This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region.</AbstractText><AbstractText Label="Methods" NlmCategory="UNASSIGNED">46 respiratory specimens were tested using the qualitative one-step qRT-PCR <i>GeneSig Human Coronavirus 2012</i> (<i>MERS</i>) kit (PrimerDesign™).</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive.</AbstractText><AbstractText Label="Conclusion" NlmCategory="UNASSIGNED">The <i>GeneSig Human Coronavirus 2012</i> (<i>MERS</i>) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions.</AbstractText><CopyrightInformation>© 2016 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hoteit</LastName><ForeName>Rouba</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shammaa</LastName><ForeName>Dina</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahfouz</LastName><ForeName>Rami</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, 1107-2020, Lebanon.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Gene Rep</MedlineTA><NlmUniqueID>101680713</NlmUniqueID><ISSNLinking>2452-0144</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ACTB, Actin Beta</Keyword><Keyword MajorTopicYN="N">DNA, Deoxyribonucleic Acid</Keyword><Keyword MajorTopicYN="N">GeneSig kit</Keyword><Keyword MajorTopicYN="N">Lebanon</Keyword><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">MERS-CoV, Middle East Respiratory Syndrome</Keyword><Keyword MajorTopicYN="N">ORF, Open Reading Frame</Keyword><Keyword MajorTopicYN="N">RNA, Ribonucleic Acid</Keyword><Keyword MajorTopicYN="N">RT-PCR, Reverse-Transcriptase Polymerase Chain Reaction</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>04</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32289095</ArticleId><ArticleId IdType="doi">10.1016/j.genrep.2016.04.004</ArticleId><ArticleId IdType="pii">S2452-0144(16)30021-8</ArticleId><ArticleId IdType="pmc">PMC7104146</ArticleId></ArticleIdList><pmc-dir>pmcsd</pmc-dir>
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