H2N2V1, Main, Curation, bibRecord, 001F19

Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus

Identifieur interne : 001F19 ( Main/Curation ); précédent : 001F18; suivant : 001F20

Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus

Auteurs : M. L. Herlocher [États-Unis] ; H. F. Maassab ; R. G. Webster [États-Unis]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1993.

RBID : Pascal:93-0625644

Descripteurs français

KwdFr :
- ARN viral (), Adaptation physiologique, Animaux, Basse température, Chiens, Femelle, Furets, Mutation, Sous-type H2N2 du virus de la grippe A, Séquence nucléotidique, Virus de la grippe A (génétique), Virus de la grippe A (physiologie).
MESH :
- génétique : Virus de la grippe A.
- physiologie : Virus de la grippe A.
Pascal (Inist)
- ARN viral, Adaptation physiologique, Animaux, Basse température, Chiens, Femelle, Furets, Influenzavirus A, Mutation, Souche vaccinale, Adaptation, Froid, Etude comparative, Gène, Mutation, RNA, Sous-type H2N2 du virus de la grippe A, Structure secondaire, Séquence nucléotidique.

English descriptors

KwdEn :
- Adaptation, Adaptation, Physiological, Animals, Base Sequence, Cold, Cold Temperature, Comparative study, Dogs, Female, Ferrets, Gene, Influenza A Virus, H2N2 Subtype, Influenza A virus (genetics), Influenza A virus (physiology), Influenzavirus A, Mutation, RNA, RNA, Viral (chemistry), Secondary structure, Vaccine strain.
MESH :
- chemical , chemistry : RNA, Viral.
- genetics : Influenza A virus.
- physiology : Influenza A virus.
- Adaptation, Physiological, Animals, Base Sequence, Cold Temperature, Dogs, Female, Ferrets, Influenza A Virus, H2N2 Subtype, Mutation.

Abstract

The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2)

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Pascal:93-0625644

Le document en format XML

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<term>Furets</term>
<term>Mutation</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.</div>
</front>
</TEI>
</PubMed>
</double>
</record>

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Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus

Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus

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