Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.
Identifieur interne : 000384 ( PubMed/Checkpoint ); précédent : 000383; suivant : 000385Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.
Auteurs : M L Herlocher [États-Unis] ; H F Maassab ; R G WebsterSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1993.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus de la grippe A.
- physiologie : Virus de la grippe A.
- ARN viral, Adaptation physiologique, Animaux, Basse température, Chiens, Femelle, Furets, Mutation, Sous-type H2N2 du virus de la grippe A, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA, Viral.
- genetics : Influenza A virus.
- physiology : Influenza A virus.
- Adaptation, Physiological, Animals, Base Sequence, Cold Temperature, Dogs, Female, Ferrets, Influenza A Virus, H2N2 Subtype, Mutation.
Abstract
The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.
DOI: 10.1073/pnas.90.13.6032
PubMed: 8327480
Affiliations:
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type="abstract" xml:lang="en">The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8327480</PMID><DateCompleted><Year>1993</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>90</Volume><Issue>13</Issue><PubDate><Year>1993</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.</ArticleTitle><Pagination><MedlinePgn>6032-6</MedlinePgn></Pagination><Abstract><AbstractText>The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Herlocher</LastName><ForeName>M L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38101.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maassab</LastName><ForeName>H F</ForeName><Initials>HF</Initials></Author><Author ValidYN="Y"><LastName>Webster</LastName><ForeName>R G</ForeName><Initials>RG</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1-A1-52564</GrantID><Agency>PHS HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>AI-20591</GrantID><Acronym>AI</Acronym><Agency>NIAID 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IdType="pubmed">5544140</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Herlocher, M L" sort="Herlocher, M L" uniqKey="Herlocher M" first="M L" last="Herlocher">M L Herlocher</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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