Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.
Identifieur interne : 001F40 ( Main/Merge ); précédent : 001F39; suivant : 001F41Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.
Auteurs : M L Herlocher [États-Unis] ; H F Maassab ; R G WebsterSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1993.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus de la grippe A.
- physiologie : Virus de la grippe A.
- ARN viral, Adaptation physiologique, Animaux, Basse température, Chiens, Femelle, Furets, Mutation, Sous-type H2N2 du virus de la grippe A, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA, Viral.
- genetics : Influenza A virus.
- physiology : Influenza A virus.
- Adaptation, Physiological, Animals, Base Sequence, Cold Temperature, Dogs, Female, Ferrets, Influenza A Virus, H2N2 Subtype, Mutation.
Abstract
The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.
DOI: 10.1073/pnas.90.13.6032
PubMed: 8327480
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000423
- to stream PubMed, to step Curation: 000423
- to stream PubMed, to step Checkpoint: 000384
- to stream Ncbi, to step Merge: 001314
- to stream Ncbi, to step Curation: 001314
- to stream Ncbi, to step Checkpoint: 001314
Links to Exploration step
pubmed:8327480Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.</title><author><name sortKey="Herlocher, M L" sort="Herlocher, M L" uniqKey="Herlocher M" first="M L" last="Herlocher">M L Herlocher</name><affiliation wicri:level="2"><nlm:affiliation>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38101.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name></author><author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8327480</idno><idno type="pmid">8327480</idno><idno type="doi">10.1073/pnas.90.13.6032</idno><idno type="wicri:Area/PubMed/Corpus">000423</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000423</idno><idno type="wicri:Area/PubMed/Curation">000423</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000423</idno><idno type="wicri:Area/PubMed/Checkpoint">000384</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000384</idno><idno type="wicri:Area/Ncbi/Merge">001314</idno><idno type="wicri:Area/Ncbi/Curation">001314</idno><idno type="wicri:Area/Ncbi/Checkpoint">001314</idno><idno type="wicri:doubleKey">0027-8424:1993:Herlocher M:molecular:and:biological</idno><idno type="wicri:Area/Main/Merge">001F40</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.</title><author><name sortKey="Herlocher, M L" sort="Herlocher, M L" uniqKey="Herlocher M" first="M L" last="Herlocher">M L Herlocher</name><affiliation wicri:level="2"><nlm:affiliation>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38101.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name></author><author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Base Sequence</term><term>Cold Temperature</term><term>Dogs</term><term>Female</term><term>Ferrets</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (physiology)</term><term>Mutation</term><term>RNA, Viral (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Chiens</term><term>Femelle</term><term>Furets</term><term>Mutation</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Séquence nucléotidique</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Base Sequence</term><term>Cold Temperature</term><term>Dogs</term><term>Female</term><term>Ferrets</term><term>Influenza A Virus, H2N2 Subtype</term><term>Mutation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Chiens</term><term>Femelle</term><term>Furets</term><term>Mutation</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes responsible for cold adaptation, we have compared the sequence of the master ca vaccine strain to its progenitor wild-type virus, wt A/AA/6/60 E2 (wt2). Only 4 nt differences encoding 2 aa differences were found in three gene segments. Computer-predicted RNA folds project different secondary structures between the ca and wt2 molecules based on the two silent differences between them. Genes coding for the acidic polymerase, matrix, and nonstructural proteins are identical between the two viruses. The few differences found in the ca A/AA/6/60 virus after its long stepwise passage at 25 degrees C in primary chicken kidney cells suggest that cold adaptation resulted in greater genetic stability for the highly variable RNA genome.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001F40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:8327480
|texte= Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:8327480" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |