Morphological and mineral analysis of dental enamel after erosive challenge in gastric juice and orange juice.
Identifieur interne : 000D26 ( Ncbi/Curation ); précédent : 000D25; suivant : 000D27Morphological and mineral analysis of dental enamel after erosive challenge in gastric juice and orange juice.
Auteurs : Sheila Regina Maia Braga [Brésil] ; Dalva Lúcia Araújo De Faria ; Elisabeth De Oliveira ; Maria Angela Pita SobralSource :
- Microscopy research and technique [ 1097-0029 ] ; 2011.
English descriptors
- KwdEn :
- Beverages (toxicity), Carbonates (analysis), Caustics (toxicity), Citrus sinensis (chemistry), Dental Enamel (chemistry), Dental Enamel (drug effects), Dental Enamel (ultrastructure), Gastric Juice (chemistry), Humans, Microscopy, Electron, Scanning, Minerals (analysis), Phosphates (analysis), Spectrophotometry, Atomic, Spectrum Analysis, Raman.
- MESH :
- chemical , analysis : Carbonates, Minerals, Phosphates.
- chemistry : Citrus sinensis, Dental Enamel, Gastric Juice.
- drug effects : Dental Enamel.
- toxicity : Beverages, Caustics.
- ultrastructure : Dental Enamel.
- Humans, Microscopy, Electron, Scanning, Spectrophotometry, Atomic, Spectrum Analysis, Raman.
Abstract
This study evaluated and compared in vitro the morphology and mineral composition of dental enamel after erosive challenge in gastric juice and orange juice. Human enamel specimens were submitted to erosive challenge using gastric juice (from endoscopy exam) (n = 10), and orange juice (commercially-available) (n = 10), as follows: 5 min in 3 mL of demineralization solution, rinse with distilled water, and store in artificial saliva for 3 h. This cycle was repeated four times a day for 14 days. Calcium (Ca) loss after acid exposure was determined by atomic emission spectroscopy. The presence of carbonate (CO) and phosphate (PO) in the specimens was evaluated before and after the erosive challenge by FT-Raman spectroscopy. Data were tested using t-tests (P < 0.05). Morphology of enamel was observed in scanning electron microscopy (SEM). The mean loss of Ca was: 12.74 ± 3.33 mg/L Ca (gastric juice) and 7.07 ± 1.44 mg/L Ca (orange juice). The analysis by atomic emission spectroscopy showed statistically significant difference between erosive potential of juices (P = 0.0003). FT-Raman spectroscopy found no statistically significant difference in the ratio CO/PO after the erosive challenge. The CO/PO ratios values before and after the challenge were: 0.16/0.17 (gastric juice) (P = 0.37) and 0.18/0.14 (orange juice) (P = 0.16). Qualitative analysis by SEM showed intense alterations of enamel surface. The gastric juice caused more changes in morphology and mineral composition of dental enamel than orange juice. The atomic emission spectroscopy showed to be more suitable to analyze small mineral loss after erosive challenge than FT-Raman.
DOI: 10.1002/jemt.20998
PubMed: 21538693
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Human enamel specimens were submitted to erosive challenge using gastric juice (from endoscopy exam) (n = 10), and orange juice (commercially-available) (n = 10), as follows: 5 min in 3 mL of demineralization solution, rinse with distilled water, and store in artificial saliva for 3 h. This cycle was repeated four times a day for 14 days. Calcium (Ca) loss after acid exposure was determined by atomic emission spectroscopy. The presence of carbonate (CO) and phosphate (PO) in the specimens was evaluated before and after the erosive challenge by FT-Raman spectroscopy. Data were tested using t-tests (P < 0.05). Morphology of enamel was observed in scanning electron microscopy (SEM). The mean loss of Ca was: 12.74 ± 3.33 mg/L Ca (gastric juice) and 7.07 ± 1.44 mg/L Ca (orange juice). The analysis by atomic emission spectroscopy showed statistically significant difference between erosive potential of juices (P = 0.0003). FT-Raman spectroscopy found no statistically significant difference in the ratio CO/PO after the erosive challenge. The CO/PO ratios values before and after the challenge were: 0.16/0.17 (gastric juice) (P = 0.37) and 0.18/0.14 (orange juice) (P = 0.16). Qualitative analysis by SEM showed intense alterations of enamel surface. The gastric juice caused more changes in morphology and mineral composition of dental enamel than orange juice. The atomic emission spectroscopy showed to be more suitable to analyze small mineral loss after erosive challenge than FT-Raman.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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