Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Identifieur interne : 003869 ( Main/Exploration ); précédent : 003868; suivant : 003870Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Auteurs : C. Maire [France] ; M. Bouchy ; M. Donner ; J C AndréSource :
- Biorheology [ 0006-355X ]
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : 1,6-Diphényl-hexa-1,3,5-triène.
- métabolisme : Membrane érythrocytaire.
- pharmacocinétique : 1,6-Diphényl-hexa-1,3,5-triène, Colorants fluorescents.
- Cinétique, Humains, Mathématiques, Modèles biologiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Diphenylhexatriene.
- chemical , pharmacokinetics : Diphenylhexatriene, Fluorescent Dyes.
- metabolism : Erythrocyte Membrane.
- Humans, Kinetics, Mathematics, Models, Biological.
Abstract
A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
PubMed: 1306379
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000256
- to stream PubMed, to step Curation: 000256
- to stream PubMed, to step Checkpoint: 000256
- to stream Ncbi, to step Merge: 000039
- to stream Ncbi, to step Curation: 000039
- to stream Ncbi, to step Checkpoint: 000039
- to stream Main, to step Merge: 004081
- to stream Main, to step Curation: 003869
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.</title>
<author><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
<affiliation wicri:level="3"><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>LA 328 du CNRS, ENSIC, Nancy</wicri:regionArea>
<placeName><region type="region">Grand Est</region>
<region type="old region">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
</author>
<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
</author>
<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="????"><PubDate><MedlineDate>1992 Sep-Dec</MedlineDate>
</PubDate>
</date>
<idno type="RBID">pubmed:1306379</idno>
<idno type="pmid">1306379</idno>
<idno type="wicri:Area/PubMed/Corpus">000256</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000256</idno>
<idno type="wicri:Area/PubMed/Curation">000256</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000256</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000256</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000256</idno>
<idno type="wicri:Area/Ncbi/Merge">000039</idno>
<idno type="wicri:Area/Ncbi/Curation">000039</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000039</idno>
<idno type="wicri:doubleKey">0006-355X::Maire C:membrane:labelling:by</idno>
<idno type="wicri:Area/Main/Merge">004081</idno>
<idno type="wicri:Area/Main/Curation">003869</idno>
<idno type="wicri:Area/Main/Exploration">003869</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.</title>
<author><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
<affiliation wicri:level="3"><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>LA 328 du CNRS, ENSIC, Nancy</wicri:regionArea>
<placeName><region type="region">Grand Est</region>
<region type="old region">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
</author>
<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
</author>
<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
</author>
</analytic>
<series><title level="j">Biorheology</title>
<idno type="ISSN">0006-355X</idno>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Diphenylhexatriene (analogs & derivatives)</term>
<term>Diphenylhexatriene (pharmacokinetics)</term>
<term>Erythrocyte Membrane (metabolism)</term>
<term>Fluorescent Dyes (pharmacokinetics)</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène (analogues et dérivés)</term>
<term>1,6-Diphényl-hexa-1,3,5-triène (pharmacocinétique)</term>
<term>Cinétique</term>
<term>Colorants fluorescents (pharmacocinétique)</term>
<term>Humains</term>
<term>Mathématiques</term>
<term>Membrane érythrocytaire (métabolisme)</term>
<term>Modèles biologiques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Diphenylhexatriene</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Diphenylhexatriene</term>
<term>Fluorescent Dyes</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythrocyte Membrane</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Membrane érythrocytaire</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacocinétique" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term>
<term>Colorants fluorescents</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term>
<term>Humains</term>
<term>Mathématiques</term>
<term>Modèles biologiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Grand Est</li>
<li>Lorraine (région)</li>
</region>
<settlement><li>Nancy</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
<name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
<name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
</noCountry>
<country name="France"><region name="Grand Est"><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003869 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003869 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Lorraine |area= LrgpV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:1306379 |texte= Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:1306379" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a LrgpV1
![]() | This area was generated with Dilib version V0.6.32. | ![]() |