Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Identifieur interne : 004081 ( Main/Merge ); précédent : 004080; suivant : 004082Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Auteurs : C. Maire [France] ; M. Bouchy ; M. Donner ; J C AndréSource :
- Biorheology [ 0006-355X ]
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : 1,6-Diphényl-hexa-1,3,5-triène.
- métabolisme : Membrane érythrocytaire.
- pharmacocinétique : 1,6-Diphényl-hexa-1,3,5-triène, Colorants fluorescents.
- Cinétique, Humains, Mathématiques, Modèles biologiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Diphenylhexatriene.
- chemical , pharmacokinetics : Diphenylhexatriene, Fluorescent Dyes.
- metabolism : Erythrocyte Membrane.
- Humans, Kinetics, Mathematics, Models, Biological.
Abstract
A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
PubMed: 1306379
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pubmed:1306379Le document en format XML
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Donner</name></author><author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name></author></analytic><series><title level="j">Biorheology</title><idno type="ISSN">0006-355X</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Diphenylhexatriene (analogs & derivatives)</term><term>Diphenylhexatriene (pharmacokinetics)</term><term>Erythrocyte Membrane (metabolism)</term><term>Fluorescent Dyes (pharmacokinetics)</term><term>Humans</term><term>Kinetics</term><term>Mathematics</term><term>Models, Biological</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène (analogues et dérivés)</term><term>1,6-Diphényl-hexa-1,3,5-triène (pharmacocinétique)</term><term>Cinétique</term><term>Colorants fluorescents (pharmacocinétique)</term><term>Humains</term><term>Mathématiques</term><term>Membrane érythrocytaire (métabolisme)</term><term>Modèles biologiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Diphenylhexatriene</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Diphenylhexatriene</term><term>Fluorescent Dyes</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythrocyte Membrane</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Membrane érythrocytaire</term></keywords><keywords scheme="MESH" qualifier="pharmacocinétique" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term><term>Colorants fluorescents</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Kinetics</term><term>Mathematics</term><term>Models, Biological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Humains</term><term>Mathématiques</term><term>Modèles biologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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