Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Identifieur interne : 000256 ( PubMed/Corpus ); précédent : 000255; suivant : 000257Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Auteurs : C. Maire ; M. Bouchy ; M. Donner ; J C AndréSource :
- Biorheology [ 0006-355X ]
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Diphenylhexatriene.
- chemical , pharmacokinetics : Diphenylhexatriene, Fluorescent Dyes.
- metabolism : Erythrocyte Membrane.
- Humans, Kinetics, Mathematics, Models, Biological.
Abstract
A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
PubMed: 1306379
Links to Exploration step
pubmed:1306379Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.</title>
<author><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
<affiliation><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
</author>
<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
</author>
<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="????"><PubDate><MedlineDate>1992 Sep-Dec</MedlineDate>
</PubDate>
</date>
<idno type="RBID">pubmed:1306379</idno>
<idno type="pmid">1306379</idno>
<idno type="wicri:Area/PubMed/Corpus">000256</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000256</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.</title>
<author><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
<affiliation><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
</author>
<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
</author>
<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
</author>
</analytic>
<series><title level="j">Biorheology</title>
<idno type="ISSN">0006-355X</idno>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Diphenylhexatriene (analogs & derivatives)</term>
<term>Diphenylhexatriene (pharmacokinetics)</term>
<term>Erythrocyte Membrane (metabolism)</term>
<term>Fluorescent Dyes (pharmacokinetics)</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Diphenylhexatriene</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Diphenylhexatriene</term>
<term>Fluorescent Dyes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythrocyte Membrane</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.</div>
</front>
</TEI>
<pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">1306379</PMID>
<DateCreated><Year>1993</Year>
<Month>08</Month>
<Day>12</Day>
</DateCreated>
<DateCompleted><Year>1993</Year>
<Month>08</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2004</Year>
<Month>11</Month>
<Day>17</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-355X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>29</Volume>
<Issue>5-6</Issue>
<PubDate><MedlineDate>1992 Sep-Dec</MedlineDate>
</PubDate>
</JournalIssue>
<Title>Biorheology</Title>
<ISOAbbreviation>Biorheology</ISOAbbreviation>
</Journal>
<ArticleTitle>Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.</ArticleTitle>
<Pagination><MedlinePgn>507-19</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Maire</LastName>
<ForeName>C</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bouchy</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Donner</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>André</LastName>
<ForeName>J C</ForeName>
<Initials>JC</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>ENGLAND</Country>
<MedlineTA>Biorheology</MedlineTA>
<NlmUniqueID>0372526</NlmUniqueID>
<ISSNLinking>0006-355X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>1720-32-7</RegistryNumber>
<NameOfSubstance UI="D004161">Diphenylhexatriene</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>71316-28-4</RegistryNumber>
<NameOfSubstance UI="C033628">1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004161">Diphenylhexatriene</DescriptorName>
<QualifierName MajorTopicYN="Y" UI="Q000031">analogs & derivatives</QualifierName>
<QualifierName MajorTopicYN="N" UI="Q000493">pharmacokinetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D004910">Erythrocyte Membrane</DescriptorName>
<QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D005456">Fluorescent Dyes</DescriptorName>
<QualifierName MajorTopicYN="Y" UI="Q000493">pharmacokinetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D007700">Kinetics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D008433">Mathematics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName MajorTopicYN="N" UI="D008954">Models, Biological</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year>
<Month>9</Month>
<Day>1</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1992</Year>
<Month>9</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1992</Year>
<Month>9</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">1306379</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000256 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000256 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Lorraine |area= LrgpV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:1306379 |texte= Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:1306379" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a LrgpV1
![]() | This area was generated with Dilib version V0.6.32. | ![]() |