Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Identifieur interne : 000039 ( Ncbi/Curation ); précédent : 000038; suivant : 000040Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
Auteurs : C. Maire [France] ; M. Bouchy ; M. Donner ; J C AndréSource :
- Biorheology [ 0006-355X ]
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : 1,6-Diphényl-hexa-1,3,5-triène.
- métabolisme : Membrane érythrocytaire.
- pharmacocinétique : 1,6-Diphényl-hexa-1,3,5-triène, Colorants fluorescents.
- Cinétique, Humains, Mathématiques, Modèles biologiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Diphenylhexatriene.
- chemical , pharmacokinetics : Diphenylhexatriene, Fluorescent Dyes.
- metabolism : Erythrocyte Membrane.
- Humans, Kinetics, Mathematics, Models, Biological.
Abstract
A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
PubMed: 1306379
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pubmed:1306379Le document en format XML
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<author><name sortKey="Maire, C" sort="Maire, C" uniqKey="Maire C" first="C" last="Maire">C. Maire</name>
<affiliation wicri:level="3"><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
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<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
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<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
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<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
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<affiliation wicri:level="3"><nlm:affiliation>LA 328 du CNRS, ENSIC, Nancy, France.</nlm:affiliation>
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<author><name sortKey="Bouchy, M" sort="Bouchy, M" uniqKey="Bouchy M" first="M" last="Bouchy">M. Bouchy</name>
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<author><name sortKey="Donner, M" sort="Donner, M" uniqKey="Donner M" first="M" last="Donner">M. Donner</name>
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<author><name sortKey="Andre, J C" sort="Andre, J C" uniqKey="Andre J" first="J C" last="André">J C André</name>
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<series><title level="j">Biorheology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Diphenylhexatriene (analogs & derivatives)</term>
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<term>Erythrocyte Membrane (metabolism)</term>
<term>Fluorescent Dyes (pharmacokinetics)</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène (analogues et dérivés)</term>
<term>1,6-Diphényl-hexa-1,3,5-triène (pharmacocinétique)</term>
<term>Cinétique</term>
<term>Colorants fluorescents (pharmacocinétique)</term>
<term>Humains</term>
<term>Mathématiques</term>
<term>Membrane érythrocytaire (métabolisme)</term>
<term>Modèles biologiques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Diphenylhexatriene</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Diphenylhexatriene</term>
<term>Fluorescent Dyes</term>
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<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythrocyte Membrane</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Membrane érythrocytaire</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacocinétique" xml:lang="fr"><term>1,6-Diphényl-hexa-1,3,5-triène</term>
<term>Colorants fluorescents</term>
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<term>Kinetics</term>
<term>Mathematics</term>
<term>Models, Biological</term>
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<front><div type="abstract" xml:lang="en">A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.</div>
</front>
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