Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments
Identifieur interne : 000625 ( Ncbi/Merge ); précédent : 000624; suivant : 000626Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments
Auteurs : Fanya Zeng [République populaire de Chine] ; Ken Yan Ching Chow [République populaire de Chine] ; Chung Chau Hon [République populaire de Chine] ; Ka Man Law [République populaire de Chine] ; Chi Wai Yip [République populaire de Chine] ; Kwok Hung Chan [République populaire de Chine] ; Joseph S. Malik Peiris [République populaire de Chine] ; Frederick Chi Ching Leung [République populaire de Chine]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (immunologie), Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Immunisation, Immunité cellulaire, Immunoglobuline G (immunologie), Plasmides (génétique), Plasmides (immunologie), Production d'anticorps (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Sous-unités de protéines (immunologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins à ADN, Virus du SRAS.
- immunologie : Anticorps, Glycoprotéines membranaires, Immunoglobuline G, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Sous-unités de protéines, Vaccins à ADN, Virus du SRAS.
- Animaux, Expression des gènes, Glycoprotéine de spicule des coronavirus, Immunisation, Immunité cellulaire, Production d'anticorps, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies (immunology), Antibody Formation (drug effects), Gene Expression, Immunity, Cellular, Immunization, Immunoglobulin G (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids (genetics), Plasmids (immunology), Protein Subunits (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Immunoglobulin G, Membrane Glycoproteins, Protein Subunits, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- drug effects : Antibody Formation.
- genetics : Plasmids, SARS Virus.
- immunology : Plasmids, SARS Virus.
- Animals, Gene Expression, Immunity, Cellular, Immunization, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.
Url:
DOI: 10.1016/j.bbrc.2004.01.166
PubMed: 14985131
PubMed Central: 7111006
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PMC:7111006Le document en format XML
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Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, 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University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S. Malik" last="Peiris">Joseph S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemical and Biophysical Research Communications</title><idno type="ISSN">0006-291X</idno><idno type="eISSN">1090-2104</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (immunology)</term><term>Antibody Formation (drug effects)</term><term>Gene Expression</term><term>Immunity, Cellular</term><term>Immunization</term><term>Immunoglobulin G (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Plasmids (genetics)</term><term>Plasmids (immunology)</term><term>Protein Subunits (immunology)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps (immunologie)</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Immunisation</term><term>Immunité cellulaire</term><term>Immunoglobuline G (immunologie)</term><term>Plasmides (génétique)</term><term>Plasmides (immunologie)</term><term>Production d'anticorps ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sous-unités de protéines (immunologie)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Immunoglobulin G</term><term>Membrane Glycoproteins</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Antibody Formation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Glycoprotéines membranaires</term><term>Immunoglobuline G</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Sous-unités de protéines</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Expression</term><term>Immunity, Cellular</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunisation</term><term>Immunité cellulaire</term><term>Production d'anticorps</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhong, N S" uniqKey="Zhong N">N.S Zhong</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J Zheng</name></author><author><name sortKey="Li, Y M" uniqKey="Li Y">Y.M Li</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M Poon</name></author><author><name sortKey="Xie, Z H" uniqKey="Xie Z">Z.H Xie</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Li, P H" uniqKey="Li P">P.H Li</name></author><author><name sortKey="Tan, S Y" uniqKey="Tan S">S.Y Tan</name></author><author><name sortKey="Chang, Q" uniqKey="Chang Q">Q Chang</name></author><author><name sortKey="Xie, J P" uniqKey="Xie J">J.P Xie</name></author><author><name 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Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yip, Chi Wai" sort="Yip, Chi Wai" uniqKey="Yip C" first="Chi Wai" last="Yip">Chi Wai Yip</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S. Malik" last="Peiris">Joseph S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">14985131</idno><idno type="pmc">7111006</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111006</idno><idno type="RBID">PMC:7111006</idno><idno type="doi">10.1016/j.bbrc.2004.01.166</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000E16</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E16</idno><idno type="wicri:Area/Pmc/Curation">000E16</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E16</idno><idno type="wicri:Area/Pmc/Checkpoint">001717</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001717</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments</title><author><name sortKey="Zeng, Fanya" sort="Zeng, Fanya" uniqKey="Zeng F" first="Fanya" last="Zeng">Fanya Zeng</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yip, Chi Wai" sort="Yip, Chi Wai" uniqKey="Yip C" first="Chi Wai" last="Yip">Chi Wai Yip</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S. Malik" last="Peiris">Joseph S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemical and Biophysical Research Communications</title><idno type="ISSN">0006-291X</idno><idno type="eISSN">1090-2104</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. 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Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name></author><author><name sortKey="Yip, Chi Wai" sort="Yip, Chi Wai" uniqKey="Yip C" first="Chi Wai" last="Yip">Chi Wai Yip</name></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name></author><author><name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S Malik" last="Peiris">Joseph S Malik Peiris</name></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14985131</idno><idno type="pmid">14985131</idno><idno type="doi">10.1016/j.bbrc.2004.01.166</idno><idno type="wicri:Area/PubMed/Corpus">002F40</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F40</idno><idno type="wicri:Area/PubMed/Curation">002F40</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F40</idno><idno type="wicri:Area/PubMed/Checkpoint">002D96</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D96</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.</title><author><name sortKey="Zeng, Fanya" sort="Zeng, Fanya" uniqKey="Zeng F" first="Fanya" last="Zeng">Fanya Zeng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea><wicri:noRegion>SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name></author><author><name sortKey="Yip, Chi Wai" sort="Yip, Chi Wai" uniqKey="Yip C" first="Chi Wai" last="Yip">Chi Wai Yip</name></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name></author><author><name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S Malik" last="Peiris">Joseph S Malik Peiris</name></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (immunology)</term><term>Antibody Formation (drug effects)</term><term>Gene Expression</term><term>Immunity, Cellular</term><term>Immunization</term><term>Immunoglobulin G (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Plasmids (genetics)</term><term>Plasmids (immunology)</term><term>Protein Subunits (immunology)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps (immunologie)</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Immunisation</term><term>Immunité cellulaire</term><term>Immunoglobuline G (immunologie)</term><term>Plasmides (génétique)</term><term>Plasmides (immunologie)</term><term>Production d'anticorps ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sous-unités de protéines (immunologie)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Immunoglobulin G</term><term>Membrane Glycoproteins</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Antibody Formation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Glycoprotéines membranaires</term><term>Immunoglobuline G</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Sous-unités de protéines</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Expression</term><term>Immunity, Cellular</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunisation</term><term>Immunité cellulaire</term><term>Production d'anticorps</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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