Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments
Identifieur interne : 000E16 ( Pmc/Curation ); précédent : 000E15; suivant : 000E17Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments
Auteurs : Fanya Zeng [République populaire de Chine] ; Ken Yan Ching Chow [République populaire de Chine] ; Chung Chau Hon [République populaire de Chine] ; Ka Man Law [République populaire de Chine] ; Chi Wai Yip [République populaire de Chine] ; Kwok Hung Chan [République populaire de Chine] ; Joseph S. Malik Peiris [République populaire de Chine] ; Frederick Chi Ching Leung [République populaire de Chine]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2004.
Abstract
The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.
Url:
DOI: 10.1016/j.bbrc.2004.01.166
PubMed: 14985131
PubMed Central: 7111006
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Malik" last="Peiris">Joseph S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea></affiliation></author><author><name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biochemical and Biophysical Research Communications</title><idno type="ISSN">0006-291X</idno><idno type="eISSN">1090-2104</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhong, N S" uniqKey="Zhong N">N.S Zhong</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J Zheng</name></author><author><name sortKey="Li, Y M" uniqKey="Li Y">Y.M Li</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M Poon</name></author><author><name sortKey="Xie, Z H" uniqKey="Xie Z">Z.H Xie</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Li, P H" uniqKey="Li P">P.H Li</name></author><author><name sortKey="Tan, S Y" uniqKey="Tan S">S.Y Tan</name></author><author><name sortKey="Chang, Q" uniqKey="Chang Q">Q Chang</name></author><author><name sortKey="Xie, J P" uniqKey="Xie J">J.P Xie</name></author><author><name sortKey="Liu, X Q" uniqKey="Liu X">X.Q Liu</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name></author><author><name sortKey="Li, D X" uniqKey="Li D">D.X Li</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y Yuen</name></author><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T Lai</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yam, L Y" uniqKey="Yam L">L.Y Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author><author><name sortKey="Nicholls, J" uniqKey="Nicholls J">J Nicholls</name></author><author><name sortKey="Yee, W K" uniqKey="Yee W">W.K Yee</name></author><author><name sortKey="Yan, W W" uniqKey="Yan W">W.W Yan</name></author><author><name sortKey="Cheung, M T" uniqKey="Cheung M">M.T Cheung</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C Cheng</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Tsang, D N" uniqKey="Tsang D">D.N Tsang</name></author><author><name sortKey="Yung, R W" uniqKey="Yung R">R.W Yung</name></author><author><name sortKey="Ng, T K" uniqKey="Ng T">T.K Ng</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gallagher, T M" uniqKey="Gallagher T">T.M Gallagher</name></author><author><name sortKey="Buchmeier, M J" uniqKey="Buchmeier M">M.J Buchmeier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, M M C" uniqKey="Lai M">M.M.C Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonavia, A" uniqKey="Bonavia A">A Bonavia</name></author><author><name sortKey="Zelus, B D" uniqKey="Zelus B">B.D Zelus</name></author><author><name sortKey="Wentworth, D E" uniqKey="Wentworth D">D.E Wentworth</name></author><author><name sortKey="Talbot, P J" uniqKey="Talbot P">P.J Talbot</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haijema, B J" uniqKey="Haijema B">B.J Haijema</name></author><author><name sortKey="Volders, H" uniqKey="Volders H">H Volders</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsai, J C" uniqKey="Tsai J">J.C Tsai</name></author><author><name sortKey="Zelus, B D" uniqKey="Zelus B">B.D Zelus</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V Holmes</name></author><author><name sortKey="Weiss, S R" uniqKey="Weiss S">S.R Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author><author><name sortKey="Davis, P J" uniqKey="Davis P">P.J Davis</name></author><author><name sortKey="Darbyshire, J H" uniqKey="Darbyshire J">J.H Darbyshire</name></author><author><name sortKey="Peters, R W" uniqKey="Peters R">R.W Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Correa, I" uniqKey="Correa I">I Correa</name></author><author><name sortKey="Gebauer, F" uniqKey="Gebauer F">F Gebauer</name></author><author><name sortKey="Bullido, M J" uniqKey="Bullido M">M.J Bullido</name></author><author><name sortKey="Sune, C" uniqKey="Sune C">C Sune</name></author><author><name sortKey="Baay, M F" uniqKey="Baay M">M.F Baay</name></author><author><name sortKey="Zwaagstra, K A" uniqKey="Zwaagstra K">K.A Zwaagstra</name></author><author><name sortKey="Posthumus, W P" uniqKey="Posthumus W">W.P Posthumus</name></author><author><name sortKey="Lenstra, J A" uniqKey="Lenstra J">J.A Lenstra</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delmas, B" uniqKey="Delmas B">B Delmas</name></author><author><name sortKey="Rasschaert, D" uniqKey="Rasschaert D">D Rasschaert</name></author><author><name sortKey="Godet, M" uniqKey="Godet M">M Godet</name></author><author><name sortKey="Gelfi, J" uniqKey="Gelfi J">J Gelfi</name></author><author><name sortKey="Laude, H" uniqKey="Laude H">H Laude</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapczynski, D R" uniqKey="Kapczynski D">D.R Kapczynski</name></author><author><name sortKey="Hilt, D A" uniqKey="Hilt D">D.A Hilt</name></author><author><name sortKey="Shapiro, D" uniqKey="Shapiro D">D Shapiro</name></author><author><name sortKey="Sellers, H S" uniqKey="Sellers H">H.S Sellers</name></author><author><name sortKey="Jackwood, M W" uniqKey="Jackwood M">M.W Jackwood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, C S" uniqKey="Song C">C.S Song</name></author><author><name sortKey="Lee, Y J" uniqKey="Lee Y">Y.J Lee</name></author><author><name sortKey="Lee, C W" uniqKey="Lee C">C.W Lee</name></author><author><name sortKey="Sung, H W" uniqKey="Sung H">H.W Sung</name></author><author><name sortKey="Kim, J H" uniqKey="Kim J">J.H Kim</name></author><author><name sortKey="Mo, I P" uniqKey="Mo I">I.P Mo</name></author><author><name sortKey="Izumiya, Y" uniqKey="Izumiya Y">Y Izumiya</name></author><author><name sortKey="Jang, J K" uniqKey="Jang J">J.K Jang</name></author><author><name sortKey="Mikami, T" uniqKey="Mikami T">T Mikami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J Bosch</name></author><author><name sortKey="Van Der Zee, R" uniqKey="Van Der Zee R">R van der Zee</name></author><author><name sortKey="De Haan, C A" uniqKey="De Haan C">C.A de Haan</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krokhin, O" uniqKey="Krokhin O">O Krokhin</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Andonov, A" uniqKey="Andonov A">A Andonov</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name></author><author><name sortKey="Flick, R" uniqKey="Flick R">R Flick</name></author><author><name sortKey="Jones, S" uniqKey="Jones S">S Jones</name></author><author><name sortKey="Stroeher, U" uniqKey="Stroeher U">U Stroeher</name></author><author><name sortKey="Bastien, N" uniqKey="Bastien N">N Bastien</name></author><author><name sortKey="Dasuri, K V" uniqKey="Dasuri K">K.V Dasuri</name></author><author><name sortKey="Cheng, K" uniqKey="Cheng K">K Cheng</name></author><author><name sortKey="Simonsen, J N" uniqKey="Simonsen J">J.N Simonsen</name></author><author><name sortKey="Perreault, H" uniqKey="Perreault H">H Perreault</name></author><author><name sortKey="Wilkins, J" uniqKey="Wilkins J">J Wilkins</name></author><author><name sortKey="Ens, W" uniqKey="Ens W">W Ens</name></author><author><name sortKey="Plummer, F" uniqKey="Plummer F">F Plummer</name></author><author><name sortKey="Standing, K G" uniqKey="Standing K">K.G Standing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kliger, Y" uniqKey="Kliger Y">Y Kliger</name></author><author><name sortKey="Levanon, E Y" uniqKey="Levanon E">E.Y Levanon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M Peiris</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Zeng, F Y" uniqKey="Zeng F">F.Y Zeng</name></author><author><name sortKey="Chan, C W M" uniqKey="Chan C">C.W.M Chan</name></author><author><name sortKey="Chan, M N" uniqKey="Chan M">M.N Chan</name></author><author><name sortKey="Chen, J D" uniqKey="Chen J">J.D Chen</name></author><author><name sortKey="Chow, K Y C" uniqKey="Chow K">K.Y.C Chow</name></author><author><name sortKey="Hon, C C" uniqKey="Hon C">C.C Hon</name></author><author><name sortKey="Hui, K H" uniqKey="Hui K">K.H Hui</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Li, V Y Y" uniqKey="Li V">V.Y.Y Li</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Leung, S W" uniqKey="Leung S">S.W Leung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Moore, M J" uniqKey="Moore M">M.J Moore</name></author><author><name sortKey="Vasilleva, N" uniqKey="Vasilleva N">N Vasilleva</name></author><author><name sortKey="Sui, J" uniqKey="Sui J">J Sui</name></author><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K Wong</name></author><author><name sortKey="Berne, M A" uniqKey="Berne M">M.A Berne</name></author><author><name sortKey="Somasundaran, M" uniqKey="Somasundaran M">M Somasundaran</name></author><author><name sortKey="Sullivan, J L" uniqKey="Sullivan J">J.L Sullivan</name></author><author><name sortKey="Luzuriage, K" uniqKey="Luzuriage K">K Luzuriage</name></author><author><name sortKey="Greenough, T C" uniqKey="Greenough T">T.C Greenough</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H Choe</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M Farzan</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Tang, X" uniqKey="Tang X">X Tang</name></author><author><name sortKey="Yin, C" uniqKey="Yin C">C Yin</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y Fu</name></author><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Jia, W" uniqKey="Jia W">W Jia</name></author><author><name sortKey="Xu, A" uniqKey="Xu A">A Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, G" uniqKey="Li G">G Li</name></author><author><name sortKey="Chen, X J" uniqKey="Chen X">X.J Chen</name></author><author><name sortKey="Xu, A L" uniqKey="Xu A">A.L Xu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gao, W" uniqKey="Gao W">W Gao</name></author><author><name sortKey="Tamin, A" uniqKey="Tamin A">A Tamin</name></author><author><name sortKey="Soloff, A" uniqKey="Soloff A">A Soloff</name></author><author><name sortKey="D Iuto, L" uniqKey="D Iuto L">L D’Aiuto</name></author><author><name sortKey="Nwanegbo, E" uniqKey="Nwanegbo E">E Nwanegbo</name></author><author><name sortKey="Robbins, P D" uniqKey="Robbins P">P.D Robbins</name></author><author><name sortKey="Bellini, W J" uniqKey="Bellini W">W.J Bellini</name></author><author><name sortKey="Barratt Boyes, S" uniqKey="Barratt Boyes S">S Barratt-Boyes</name></author><author><name sortKey="Gambotto, A" uniqKey="Gambotto A">A Gambotto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davis, H L" uniqKey="Davis H">H.L Davis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zeng, F Y" uniqKey="Zeng F">F.Y Zeng</name></author><author><name sortKey="Chan, C W M" uniqKey="Chan C">C.W.M Chan</name></author><author><name sortKey="Chan, M N" uniqKey="Chan M">M.N Chan</name></author><author><name sortKey="Chan, J D" uniqKey="Chan J">J.D Chan</name></author><author><name sortKey="Chow, K Y C" uniqKey="Chow K">K.Y.C Chow</name></author><author><name sortKey="Hon, C C" uniqKey="Hon C">C.C Hon</name></author><author><name sortKey="Hui, K H" uniqKey="Hui K">K.H Hui</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Li, V Y Y" uniqKey="Li V">V.Y.Y Li</name></author><author><name sortKey="Wang, C Y" uniqKey="Wang C">C.Y Wang</name></author><author><name sortKey="Wang, P Y" uniqKey="Wang P">P.Y Wang</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y Yuen</name></author><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M Peiris</name></author><author><name sortKey="Leung, F C C" uniqKey="Leung F">F.C.C Leung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kato, Y" uniqKey="Kato Y">Y Kato</name></author><author><name sortKey="Shimokawa, N" uniqKey="Shimokawa N">N Shimokawa</name></author><author><name sortKey="Kato, T" uniqKey="Kato T">T Kato</name></author><author><name sortKey="Hirai, T" uniqKey="Hirai T">T Hirai</name></author><author><name sortKey="Yoshihama, K" uniqKey="Yoshihama K">K Yoshihama</name></author><author><name sortKey="Kawai, H" uniqKey="Kawai H">H Kawai</name></author><author><name sortKey="Hattori, M" uniqKey="Hattori M">M Hattori</name></author><author><name sortKey="Ezashi, T" uniqKey="Ezashi T">T Ezashi</name></author><author><name sortKey="Shimogori, Y" uniqKey="Shimogori Y">Y Shimogori</name></author><author><name sortKey="Wakabayashi, K" uniqKey="Wakabayashi K">K Wakabayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Markowska Daniel, I" uniqKey="Markowska Daniel I">I Markowska-Daniel</name></author><author><name sortKey="Collins, R A" uniqKey="Collins R">R.A Collins</name></author><author><name sortKey="Pejsak, Z" uniqKey="Pejsak Z">Z Pejsak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name></author><author><name sortKey="Tu, C" uniqKey="Tu C">C Tu</name></author><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Hu, R" uniqKey="Hu R">R Hu</name></author><author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name></author><author><name sortKey="Zhang, M" uniqKey="Zhang M">M Zhang</name></author><author><name sortKey="Yin, Z" uniqKey="Yin Z">Z Yin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Torres, C A T" uniqKey="Torres C">C.A.T Torres</name></author><author><name sortKey="Yang, K" uniqKey="Yang K">K Yang</name></author><author><name sortKey="Mustafa, F" uniqKey="Mustafa F">F Mustafa</name></author><author><name sortKey="Robinson, H L" uniqKey="Robinson H">H.L Robinson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anton, I M" uniqKey="Anton I">I.M Anton</name></author><author><name sortKey="Gonzalez, S" uniqKey="Gonzalez S">S Gonzalez</name></author><author><name sortKey="Bullido, M J" uniqKey="Bullido M">M.J Bullido</name></author><author><name sortKey="Corsin, M" uniqKey="Corsin M">M Corsin</name></author><author><name sortKey="Risco, C" uniqKey="Risco C">C Risco</name></author><author><name sortKey="Langeveld, J P" uniqKey="Langeveld J">J.P Langeveld</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biochem Biophys Res Commun</journal-id><journal-id journal-id-type="iso-abbrev">Biochem. Biophys. Res. Commun</journal-id><journal-title-group><journal-title>Biochemical and Biophysical Research Communications</journal-title></journal-title-group><issn pub-type="ppub">0006-291X</issn><issn pub-type="epub">1090-2104</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">14985131</article-id><article-id pub-id-type="pmc">7111006</article-id><article-id pub-id-type="publisher-id">S0006-291X(04)00210-4</article-id><article-id pub-id-type="doi">10.1016/j.bbrc.2004.01.166</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Fanya</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Chow</surname><given-names>Ken Yan Ching</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Hon</surname><given-names>Chung Chau</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Law</surname><given-names>Ka Man</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Yip</surname><given-names>Chi Wai</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Kwok Hung</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Peiris</surname><given-names>Joseph S.Malik</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Leung</surname><given-names>Frederick Chi Ching</given-names></name><email>fcleung@hkucc.hku.hk</email><xref rid="AFF1" ref-type="aff">a</xref><xref rid="COR1" ref-type="corresp">∗</xref></contrib></contrib-group><aff id="AFF1"><label>a</label>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</aff><aff id="AFF2"><label>b</label>Department of Microbiology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China</aff><author-notes><corresp id="COR1"><label>∗</label>Corresponding author. Fax: +852-2857-4672 <email>fcleung@hkucc.hku.hk</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>12</day><month>2</month><year>2004</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>19</day><month>3</month><year>2004</year></pub-date><pub-date pub-type="epub"><day>12</day><month>2</month><year>2004</year></pub-date><volume>315</volume><issue>4</issue><fpage>1134</fpage><lpage>1139</lpage><history><date date-type="received"><day>18</day><month>1</month><year>2004</year></date></history><permissions><copyright-statement>Copyright © 2004 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2004</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18–495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>Coronavirus</kwd><kwd>Spike glycoprotein</kwd><kwd>DNA vaccine</kwd><kwd>Neutralizing antibody</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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